Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PS040_RS04040 Genome accession   NZ_CP117637
Coordinates   833871..834851 (+) Length   326 a.a.
NCBI ID   WP_273783050.1    Uniprot ID   -
Organism   Escherichia albertii strain BIA_18     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 828871..839851
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PS040_RS04010 (PS040_04010) - 829476..830483 (+) 1008 WP_273783048.1 DUF1202 family protein -
  PS040_RS04015 (PS040_04015) hemW 830548..831684 (-) 1137 WP_000239934.1 radical SAM family heme chaperone HemW -
  PS040_RS04020 (PS040_04020) - 831677..832270 (-) 594 WP_001174776.1 XTP/dITP diphosphatase -
  PS040_RS04025 (PS040_04025) yggU 832278..832568 (-) 291 WP_001277225.1 DUF167 family protein YggU -
  PS040_RS04030 (PS040_04030) yggT 832565..833131 (-) 567 WP_001094839.1 osmotic shock tolerance protein YggT -
  PS040_RS04035 (PS040_04035) yggS 833149..833853 (-) 705 WP_000997809.1 pyridoxal phosphate homeostasis protein -
  PS040_RS04040 (PS040_04040) pilT 833871..834851 (+) 981 WP_273783050.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PS040_RS04045 (PS040_04045) ruvX 835035..835451 (-) 417 WP_000017099.1 Holliday junction resolvase RuvX -
  PS040_RS04050 (PS040_04050) - 835451..836014 (-) 564 WP_002461296.1 YqgE/AlgH family protein -
  PS040_RS04055 (PS040_04055) gshB 836123..837073 (-) 951 WP_273783052.1 glutathione synthase -
  PS040_RS04060 (PS040_04060) rsmE 837086..837817 (-) 732 WP_002461297.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  PS040_RS04065 (PS040_04065) endA 837897..838604 (-) 708 WP_059224115.1 deoxyribonuclease I -
  PS040_RS04070 (PS040_04070) - 838699..839196 (-) 498 WP_054410340.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35950.11 Da        Isoelectric Point: 5.5789

>NTDB_id=786715 PS040_RS04040 WP_273783050.1 833871..834851(+) (pilT) [Escherichia albertii strain BIA_18]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDTPDIEVLLREWLDDDQRALLLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPTVLPELLNNENGLILVTGATGSGKSTTLAAMVGYLNQHTDAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELCDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=786715 PS040_RS04040 WP_273783050.1 833871..834851(+) (pilT) [Escherichia albertii strain BIA_18]
ATGAATATGGAAGAAATTGTGGCTCTTAGTGTAAAGCATAATGTCTCGGATCTACACCTGTGTAGCGCCTGGCCTGCACG
CTGGCGTATTCGCGGGAGGATGGAAGCTGCGCCGTTTGATACGCCGGACATTGAAGTGCTGCTAAGGGAATGGCTGGATG
ACGATCAGCGAGCGTTATTGCTGGAGAATGGGCAGTTGGACTTTGCCGTGTCGTTGGCGGAAAACCAGCGATTGCGCGGC
AGTGCGTTCGCGCAACGGCAAGGCATTTCGCTGGCGTTACGGTTGTTGCCTTCGCATTGCCCGCAACTCGAACAGCTTGG
TGCGCCGACGGTATTACCGGAACTACTCAATAACGAGAATGGACTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CCACCACACTGGCGGCGATGGTTGGTTATCTCAATCAGCATACCGATGCGCATATTCTTACGCTGGAAGATCCTGTGGAA
TATCTTTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAGATTGGTTTGCACTGTATGACGTTTGCATCGGGATTACG
TGCGGCATTGCGGGAAGATCCCGATGTAATTTTGCTTGGCGAGCTGTGTGACAGCGAGACCATTCGCCTGGCGTTGACGG
CGGCAGAAACAGGTCATCTGGTGCTGGCAACCTTGCATACGCGCGGAGCGGCTCAGGCGGTCGAACGGCTGGTGGATTCG
TTTCCGGCGCAGGAAAAAGATCCCGTGCGTAATCAACTGGCGGGTAGTTTACGGGCCGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTGCTGATTAACACACCTGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTGCCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACTTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(61-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.235

100

0.494
  pilT Vibrio cholerae strain A1552

49.235

100

0.494
  pilT Neisseria meningitidis 8013

47.256

100

0.475
  pilT Neisseria gonorrhoeae MS11

46.951

100

0.472
  pilT Acinetobacter baylyi ADP1

46.177

100

0.463
  pilT Acinetobacter baumannii D1279779

45.872

100

0.46
  pilT Acinetobacter baumannii strain A118

45.872

100

0.46
  pilT Acinetobacter nosocomialis M2

45.872

100

0.46
  pilT Pseudomonas stutzeri DSM 10701

45.872

100

0.46
  pilT Pseudomonas aeruginosa PAK

45.566

100

0.457
  pilT Legionella pneumophila strain ERS1305867

44.343

100

0.445
  pilT Legionella pneumophila strain Lp02

44.343

100

0.445
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.509

97.546

0.405
  pilU Vibrio cholerae strain A1552

40.063

97.239

0.39
  pilU Pseudomonas stutzeri DSM 10701

37.952

100

0.387