Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PS035_RS04070 Genome accession   NZ_CP117570
Coordinates   840554..841534 (+) Length   326 a.a.
NCBI ID   WP_273817707.1    Uniprot ID   -
Organism   Escherichia albertii strain BIA_50     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 835554..846534
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PS035_RS04040 (PS035_04040) - 836159..837166 (+) 1008 WP_273817706.1 DUF1202 family protein -
  PS035_RS04045 (PS035_04045) hemW 837231..838367 (-) 1137 WP_059218090.1 radical SAM family heme chaperone HemW -
  PS035_RS04050 (PS035_04050) - 838360..838953 (-) 594 WP_001174776.1 XTP/dITP diphosphatase -
  PS035_RS04055 (PS035_04055) yggU 838961..839251 (-) 291 WP_001277225.1 DUF167 family protein YggU -
  PS035_RS04060 (PS035_04060) yggT 839248..839814 (-) 567 WP_001094839.1 osmotic shock tolerance protein YggT -
  PS035_RS04065 (PS035_04065) yggS 839832..840536 (-) 705 WP_000997809.1 pyridoxal phosphate homeostasis protein -
  PS035_RS04070 (PS035_04070) pilT 840554..841534 (+) 981 WP_273817707.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PS035_RS04075 (PS035_04075) ruvX 841718..842134 (-) 417 WP_000017099.1 Holliday junction resolvase RuvX -
  PS035_RS04080 (PS035_04080) - 842134..842697 (-) 564 WP_002461296.1 YqgE/AlgH family protein -
  PS035_RS04085 (PS035_04085) gshB 842806..843756 (-) 951 WP_273809617.1 glutathione synthase -
  PS035_RS04090 (PS035_04090) rsmE 843769..844500 (-) 732 WP_059228104.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  PS035_RS04095 (PS035_04095) endA 844580..845287 (-) 708 WP_002461298.1 deoxyribonuclease I -
  PS035_RS04100 (PS035_04100) - 845382..845879 (-) 498 WP_002461299.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36047.17 Da        Isoelectric Point: 5.5860

>NTDB_id=786428 PS035_RS04070 WP_273817707.1 840554..841534(+) (pilT) [Escherichia albertii strain BIA_50]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDTPDVEELLREWLDDDQRALLLENGQLDFAVSLVENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPTVLPELLNNENGLILVTGATGSGKSTTLAAMVGYLNQHTDAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=786428 PS035_RS04070 WP_273817707.1 840554..841534(+) (pilT) [Escherichia albertii strain BIA_50]
ATGAATATGGAAGAAATTGTGGCTCTTAGTGTAAAGCATAATGTCTCGGATCTACACCTGTGTAGCGCCTGGCCTGCACG
CTGGCGTATTCGCGGGAGGATGGAAGCTGCGCCGTTTGATACGCCGGACGTTGAAGAGCTGCTAAGGGAATGGCTGGATG
ACGATCAGCGAGCGTTATTGCTGGAGAATGGGCAGTTGGATTTTGCCGTGTCGTTGGTGGAAAACCAGCGATTGCGCGGC
AGTGCGTTCGCGCAACGGCAAGGCATTTCGCTGGCGTTACGGTTGTTGCCTTCGCATTGTCCGCAACTCGAACAACTTGG
TGCGCCGACGGTATTACCGGAACTACTCAATAACGAGAATGGACTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CCACCACACTGGCGGCGATGGTTGGTTATCTCAATCAGCATACCGATGCGCATATTCTTACGCTGGAAGATCCTGTGGAA
TATCTTTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAGATTGGTTTGCACTGTATGACGTTTGCATCGGGATTACG
TGCGGCATTACGGGAAGATCCCGATGTAATTTTGCTTGGCGAGCTGCGTGACAGCGAGACCATTCGCTTGGCGTTGACGG
CGGCAGAAACAGGTCATCTGGTGCTGGCAACCTTGCATACGCGCGGAGCAGCTCAGGCGGTCGAGCGGCTGGTGGATTCG
TTTCCGGCGCAGGAAAAAGATCCCGTGCGTAATCAACTGGCGGGTAGTTTACGGGCCGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTGCTGATTAACACACCTGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTGCCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACTTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(63-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.847

100

0.5
  pilT Vibrio cholerae strain A1552

49.847

100

0.5
  pilT Neisseria meningitidis 8013

47.866

100

0.482
  pilT Neisseria gonorrhoeae MS11

47.561

100

0.479
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Acinetobacter baumannii D1279779

46.483

100

0.466
  pilT Acinetobacter baumannii strain A118

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

46.483

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

46.483

100

0.466
  pilT Pseudomonas aeruginosa PAK

46.177

100

0.463
  pilT Legionella pneumophila strain ERS1305867

44.648

100

0.448
  pilT Legionella pneumophila strain Lp02

44.648

100

0.448
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.824

97.546

0.408
  pilU Vibrio cholerae strain A1552

40.694

97.239

0.396
  pilU Pseudomonas stutzeri DSM 10701

38.554

100

0.393