Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PS032_RS04735 Genome accession   NZ_CP117556
Coordinates   954039..955019 (+) Length   326 a.a.
NCBI ID   WP_010336949.1    Uniprot ID   -
Organism   Escherichia albertii strain BIA_89-5     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 949039..960019
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PS032_RS04705 (PS032_04705) - 949644..950651 (+) 1008 WP_025238393.1 DUF1202 family protein -
  PS032_RS04710 (PS032_04710) hemW 950716..951852 (-) 1137 WP_059270889.1 radical SAM family heme chaperone HemW -
  PS032_RS04715 (PS032_04715) - 951845..952438 (-) 594 WP_001174776.1 XTP/dITP diphosphatase -
  PS032_RS04720 (PS032_04720) yggU 952446..952736 (-) 291 WP_001277225.1 DUF167 family protein YggU -
  PS032_RS04725 (PS032_04725) yggT 952733..953299 (-) 567 WP_001094839.1 osmotic shock tolerance protein YggT -
  PS032_RS04730 (PS032_04730) yggS 953317..954021 (-) 705 WP_000997809.1 pyridoxal phosphate homeostasis protein -
  PS032_RS04735 (PS032_04735) pilT 954039..955019 (+) 981 WP_010336949.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PS032_RS04740 (PS032_04740) ruvX 955203..955619 (-) 417 WP_000017099.1 Holliday junction resolvase RuvX -
  PS032_RS04745 (PS032_04745) - 955619..956182 (-) 564 WP_137650140.1 YqgE/AlgH family protein -
  PS032_RS04750 (PS032_04750) gshB 956291..957241 (-) 951 WP_137650141.1 glutathione synthase -
  PS032_RS04755 (PS032_04755) rsmE 957254..957985 (-) 732 WP_137650142.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  PS032_RS04760 (PS032_04760) endA 958065..958772 (-) 708 WP_059224115.1 deoxyribonuclease I -
  PS032_RS04765 (PS032_04765) - 958867..959364 (-) 498 WP_054410340.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36019.12 Da        Isoelectric Point: 5.5860

>NTDB_id=786341 PS032_RS04735 WP_010336949.1 954039..955019(+) (pilT) [Escherichia albertii strain BIA_89-5]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDTPDVEELLREWLDDDQRALLLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPTVLPELLNNENGLILVTGATGSGKSTTLAAMVGYLNQHTDAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=786341 PS032_RS04735 WP_010336949.1 954039..955019(+) (pilT) [Escherichia albertii strain BIA_89-5]
ATGAATATGGAAGAAATTGTGGCTCTTAGTGTAAAGCATAATGTCTCGGATCTACACCTGTGTAGCGCCTGGCCTGCACG
CTGGCGTATTCGCGGGAGGATGGAAGCTGCGCCGTTTGATACGCCGGACGTTGAAGAGCTGCTAAGGGAATGGCTGGATG
ACGATCAGCGAGCGTTATTGCTGGAGAATGGGCAGTTGGATTTTGCCGTGTCGTTGGCGGAAAACCAGCGATTGCGCGGC
AGTGCGTTCGCGCAACGGCAAGGCATTTCGCTGGCGTTACGGTTGTTGCCTTCGCATTGCCCGCAACTCGAACAGCTTGG
CGCGCCGACGGTATTACCGGAACTACTCAATAACGAGAATGGACTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CCACCACACTGGCGGCGATGGTTGGTTATCTCAATCAGCATACCGATGCGCATATTCTTACGCTGGAAGATCCTGTGGAA
TATCTTTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAGATTGGTTTGCACTGTATGACGTTTGCATCGGGATTACG
TGCGGCATTGCGGGAAGATCCCGATGTAATTTTGCTTGGCGAGCTGCGTGACAGCGAGACCATTCGTCTGGCGTTGACGG
CGGCAGAAACAGGTCATCTGGTGCTGGCAACCTTGCATACGCGCGGAGCGGCTCAGGCGGTCGAGCGGCTGGTGGATTCG
TTTCCGGCGCAGGAAAAAGATCCCGTGCGTAATCAACTGGCGGGTAGTTTACGGGCCGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTGCTGATTAACACACCTGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTGCCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACTTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.847

100

0.5
  pilT Vibrio cholerae strain A1552

49.847

100

0.5
  pilT Neisseria meningitidis 8013

47.866

100

0.482
  pilT Neisseria gonorrhoeae MS11

47.561

100

0.479
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Acinetobacter baumannii D1279779

46.483

100

0.466
  pilT Acinetobacter baumannii strain A118

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

46.483

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

46.483

100

0.466
  pilT Pseudomonas aeruginosa PAK

46.177

100

0.463
  pilT Legionella pneumophila strain ERS1305867

44.954

100

0.451
  pilT Legionella pneumophila strain Lp02

44.954

100

0.451
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.824

97.546

0.408
  pilU Vibrio cholerae strain A1552

40.694

97.239

0.396
  pilU Pseudomonas stutzeri DSM 10701

38.554

100

0.393