Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GsuE55_RS01185 Genome accession   NZ_AP022557
Coordinates   266170..267225 (+) Length   351 a.a.
NCBI ID   WP_033844229.1    Uniprot ID   A0A679FIE9
Organism   Geobacillus subterraneus strain E55-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 261170..272225
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GsuE55_RS01170 (GsuE55_02400) - 262368..263144 (+) 777 WP_061912467.1 PRC-barrel domain-containing protein -
  GsuE55_RS01175 (GsuE55_02410) - 263162..264496 (+) 1335 WP_033844231.1 G5 domain-containing protein -
  GsuE55_RS01180 (GsuE55_02420) - 264493..266157 (+) 1665 WP_033844230.1 ATPase, T2SS/T4P/T4SS family -
  GsuE55_RS01185 (GsuE55_02430) pilT 266170..267225 (+) 1056 WP_033844229.1 type IV pilus twitching motility protein PilT Machinery gene
  GsuE55_RS01190 (GsuE55_02440) - 267212..268423 (+) 1212 WP_033844228.1 type II secretion system F family protein -
  GsuE55_RS01195 (GsuE55_02450) - 268517..268978 (+) 462 WP_033844227.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  GsuE55_RS01200 (GsuE55_02460) - 268997..269752 (+) 756 WP_033844226.1 A24 family peptidase -
  GsuE55_RS01205 (GsuE55_02470) pilM 269782..270717 (+) 936 WP_033844225.1 pilus assembly protein PilM -
  GsuE55_RS01210 (GsuE55_02480) - 270707..271276 (+) 570 WP_033844224.1 anti sigma-E factor RseA C-terminal domain-containing protein -
  GsuE55_RS01215 (GsuE55_02490) - 271273..271983 (+) 711 WP_061912357.1 hypothetical protein -

Sequence

Protein


Download         Length: 351 a.a.        Molecular weight: 38598.68 Da        Isoelectric Point: 7.5558

>NTDB_id=78596 GsuE55_RS01185 WP_033844229.1 266170..267225(+) (pilT) [Geobacillus subterraneus strain E55-1]
MKEKLDTLLRAAFEYRASDVHLTVGAPPIFRLNGELRLYGQERLRPEETEGMARAVIPEALWQQFQQTGELDFSYGIAGV
SRFRLNVFKQRSCVSLAVRLIPMRIPTLEELGMPSVLKRIAEKPHGLVLVTGPTGSGKSTTLAAMIDYINKTMSKHIITL
EDPIEYLHKHGKSIIDQREVGFDTGSFASGLRAALRQDPDVILVGEMRDLETIQTAITAAETGHLVLGTLHTASAPAAIE
RMIDVFPSAQQAQIRLQLASVLVAIIAQRLFPNRSRTGRAAALEILLNNAAVANLIRNEKEHQIINIMQTSRSVGMQTME
MSVKELVAAGRIDAAAVEVYLAGEREKDGAI

Nucleotide


Download         Length: 1056 bp        

>NTDB_id=78596 GsuE55_RS01185 WP_033844229.1 266170..267225(+) (pilT) [Geobacillus subterraneus strain E55-1]
ATGAAAGAAAAATTGGATACGTTGTTGCGCGCGGCGTTTGAGTACCGGGCGTCCGACGTGCATTTGACCGTCGGAGCGCC
GCCGATTTTTCGCCTGAACGGCGAGCTGAGGCTCTACGGCCAAGAGCGGCTGCGCCCTGAGGAAACGGAAGGAATGGCGC
GCGCCGTCATCCCTGAAGCGCTTTGGCAGCAGTTCCAACAAACCGGGGAGCTTGATTTTTCCTATGGGATCGCCGGCGTG
TCCCGGTTCCGCTTGAACGTGTTTAAACAGCGGTCCTGTGTTTCCTTGGCCGTTCGCCTCATCCCGATGCGCATCCCGAC
GTTGGAGGAGCTTGGCATGCCAAGCGTGCTGAAGCGCATCGCGGAAAAGCCGCACGGGCTTGTGCTCGTCACCGGGCCGA
CCGGCAGTGGCAAGTCGACGACGTTGGCCGCTATGATCGATTATATAAACAAAACGATGTCGAAGCATATTATTACGCTG
GAGGATCCGATCGAATATTTACATAAACACGGGAAATCGATCATTGATCAACGCGAGGTCGGATTTGACACCGGCAGCTT
TGCTTCCGGGCTGCGCGCCGCGCTTCGCCAAGACCCGGACGTCATTTTAGTCGGGGAGATGCGCGACTTGGAGACGATTC
AAACTGCCATTACGGCCGCGGAAACGGGGCATTTGGTGTTGGGCACGCTCCATACGGCCAGCGCCCCGGCGGCGATCGAG
CGGATGATTGACGTCTTTCCTTCGGCTCAGCAGGCGCAAATCCGCTTGCAGCTCGCTTCAGTGCTTGTCGCCATTATTGC
GCAGCGCCTGTTTCCGAACCGAAGCCGAACCGGACGGGCGGCGGCGCTCGAAATTTTACTGAACAATGCCGCGGTCGCCA
ATCTCATCCGCAACGAGAAGGAACACCAAATCATCAACATTATGCAGACGAGCCGCAGTGTGGGCATGCAGACGATGGAA
ATGAGCGTCAAGGAACTGGTTGCGGCCGGGCGGATTGATGCGGCGGCAGTTGAAGTGTACTTAGCGGGGGAGCGTGAGAA
AGATGGCGCAATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(96-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A679FIE9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

54.313

89.174

0.484
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.245

94.302

0.464
  pilT Vibrio cholerae strain A1552

49.245

94.302

0.464
  pilT Neisseria meningitidis 8013

48.632

93.732

0.456
  pilT Neisseria gonorrhoeae MS11

48.328

93.732

0.453
  pilT Pseudomonas aeruginosa PAK

49.367

90.028

0.444
  pilT Acinetobacter baylyi ADP1

47.546

92.877

0.442
  pilT Acinetobacter baumannii D1279779

47.077

92.593

0.436
  pilT Acinetobacter baumannii strain A118

47.077

92.593

0.436
  pilT Acinetobacter nosocomialis M2

46.769

92.593

0.433
  pilT Pseudomonas stutzeri DSM 10701

47.785

90.028

0.43
  pilT Legionella pneumophila strain Lp02

46.875

91.168

0.427
  pilT Legionella pneumophila strain ERS1305867

46.875

91.168

0.427
  pilU Vibrio cholerae strain A1552

42.857

93.732

0.402
  pilU Pseudomonas stutzeri DSM 10701

42.553

93.732

0.399

Multiple sequence alignment