Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PQQ05_RS03740 Genome accession   NZ_CP117326
Coordinates   776261..777241 (+) Length   326 a.a.
NCBI ID   WP_274895489.1    Uniprot ID   -
Organism   Salmonella enterica subsp. enterica serovar Newport strain RM108     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 771261..782241
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PQQ05_RS03710 (PQQ05_03710) - 771838..772845 (+) 1008 WP_000252198.1 DUF1202 domain-containing protein -
  PQQ05_RS03715 (PQQ05_03715) hemW 772937..774073 (-) 1137 WP_001096525.1 radical SAM family heme chaperone HemW -
  PQQ05_RS03720 (PQQ05_03720) - 774066..774659 (-) 594 WP_001174769.1 XTP/dITP diphosphatase -
  PQQ05_RS03725 (PQQ05_03725) yggU 774667..774957 (-) 291 WP_001277203.1 DUF167 family protein YggU -
  PQQ05_RS03730 (PQQ05_03730) - 774954..775520 (-) 567 WP_001094848.1 YggT family protein -
  PQQ05_RS03735 (PQQ05_03735) - 775539..776243 (-) 705 WP_000997812.1 YggS family pyridoxal phosphate-dependent enzyme -
  PQQ05_RS03740 (PQQ05_03740) pilT 776261..777241 (+) 981 WP_274895489.1 type IV pilus twitching motility protein PilT Machinery gene
  PQQ05_RS03745 (PQQ05_03745) - 777374..778060 (+) 687 WP_000098321.1 IclR family transcriptional regulator -
  PQQ05_RS03750 (PQQ05_03750) ruvX 778107..778523 (-) 417 WP_000017096.1 Holliday junction resolvase RuvX -
  PQQ05_RS03755 (PQQ05_03755) - 778523..779086 (-) 564 WP_001053167.1 YqgE/AlgH family protein -
  PQQ05_RS03760 (PQQ05_03760) gshB 779302..780249 (-) 948 WP_000593248.1 glutathione synthase -
  PQQ05_RS03765 (PQQ05_03765) rsmE 780269..781000 (-) 732 WP_274895490.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  PQQ05_RS03770 (PQQ05_03770) endA 781077..781784 (-) 708 WP_000286124.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36096.24 Da        Isoelectric Point: 7.5355

>NTDB_id=783818 PQQ05_RS03740 WP_274895489.1 776261..777241(+) (pilT) [Salmonella enterica subsp. enterica serovar Newport strain RM108]
MNMEEIVTLSVKHNVSDLHLCNAWPARWRKQGRMETAPFTAPDVDRPLLDWLNDAQQYQWRTHGQLDFAVSLSGTRRLRA
SAFTHQQGTSLALRLLPERCPDLAEIQTPPIVPALLASENGLILVTGATGCGKSTTLAAMVGHLNQHADKHILTLEDPIE
YRYTSKRCLIQQREIGQHCATFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKEPVRSQLAGSLRAVLSQKLEVDRQDGRVALFELLINTPATGNLIREGKLHQLAHVIQTGQQQGMMTFAQSAQWR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=783818 PQQ05_RS03740 WP_274895489.1 776261..777241(+) (pilT) [Salmonella enterica subsp. enterica serovar Newport strain RM108]
ATGAATATGGAAGAAATTGTGACGCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGTAACGCCTGGCCTGCGCG
ATGGCGCAAACAAGGACGCATGGAGACAGCGCCTTTTACCGCGCCAGATGTCGACAGGCCTCTGCTTGATTGGCTTAATG
ACGCGCAACAATATCAGTGGCGAACGCACGGTCAGCTTGATTTCGCCGTCTCGCTGTCTGGCACGCGGCGCCTGCGCGCC
AGTGCGTTCACACATCAACAGGGAACGTCGCTGGCGCTACGGCTATTGCCCGAGCGTTGTCCTGATCTGGCGGAAATCCA
GACGCCGCCGATAGTACCCGCACTGCTTGCCAGCGAGAACGGACTGATTCTGGTCACTGGTGCTACTGGCTGCGGGAAAT
CCACTACGCTGGCGGCGATGGTGGGACATCTTAATCAACATGCCGATAAACACATCCTGACGCTGGAAGATCCTATTGAG
TACCGTTACACCAGCAAGCGTTGCCTGATACAGCAGCGGGAAATCGGCCAGCATTGTGCCACTTTTGCCGCCGGGCTGCG
CGCGGCGTTACGTGAAGATCCCGATGTCATTTTACTGGGAGAGCTGCGTGACAGCGAGACCATTCGCCTGGCGCTGACGG
CGGCGGAAACGGGGCATCTGGTACTGGCAACCCTGCATACTCGCGGTGCGGCGCAGGCGGTGGAAAGATTAGTGGATAGT
TTTCCGGCGCAGGAAAAAGAACCGGTACGCAGCCAACTGGCGGGTAGTCTGCGCGCCGTACTATCACAAAAACTGGAGGT
CGACAGACAGGATGGGCGCGTGGCGTTGTTTGAATTACTGATTAATACGCCAGCCACAGGGAATTTGATCCGTGAAGGCA
AACTCCATCAGTTGGCCCACGTGATACAAACCGGGCAGCAGCAGGGGATGATGACATTCGCCCAGAGCGCGCAATGGCGT
CAGGCGCAGGGACGACTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

48.318

100

0.485
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.318

100

0.485
  pilT Neisseria meningitidis 8013

47.256

100

0.475
  pilT Neisseria gonorrhoeae MS11

46.951

100

0.472
  pilT Acinetobacter baylyi ADP1

44.954

100

0.451
  pilT Acinetobacter baumannii strain A118

44.648

100

0.448
  pilT Acinetobacter baumannii D1279779

44.648

100

0.448
  pilT Pseudomonas aeruginosa PAK

44.648

100

0.448
  pilT Pseudomonas stutzeri DSM 10701

44.648

100

0.448
  pilT Acinetobacter nosocomialis M2

44.343

100

0.445
  pilT Legionella pneumophila strain Lp02

44.654

97.546

0.436
  pilT Legionella pneumophila strain ERS1305867

44.654

97.546

0.436
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.824

97.546

0.408
  pilU Vibrio cholerae strain A1552

38.245

97.853

0.374
  pilU Pseudomonas stutzeri DSM 10701

36.254

100

0.368