Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PIB32_RS14305 Genome accession   NZ_CP116898
Coordinates   3424478..3425629 (+) Length   383 a.a.
NCBI ID   WP_308364257.1    Uniprot ID   -
Organism   Microbulbifer sp. RZ01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3419478..3430629
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PIB32_RS14285 - 3420957..3421565 (-) 609 WP_308364254.1 YggT family protein -
  PIB32_RS14290 proC 3421719..3422546 (-) 828 WP_308364255.1 pyrroline-5-carboxylate reductase -
  PIB32_RS14295 - 3422539..3423246 (-) 708 WP_308364256.1 YggS family pyridoxal phosphate-dependent enzyme -
  PIB32_RS14300 pilT 3423333..3424367 (+) 1035 WP_183459060.1 type IV pilus twitching motility protein PilT Machinery gene
  PIB32_RS14305 pilU 3424478..3425629 (+) 1152 WP_308364257.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PIB32_RS14310 - 3425878..3426432 (+) 555 WP_308375574.1 hypothetical protein -
  PIB32_RS14315 - 3426495..3427244 (+) 750 WP_308364260.1 SDR family oxidoreductase -
  PIB32_RS14320 - 3427277..3427891 (+) 615 WP_308364261.1 LysE family translocator -
  PIB32_RS14325 - 3428021..3428839 (+) 819 WP_308364263.1 hypothetical protein -
  PIB32_RS14330 - 3429293..3430042 (-) 750 WP_308364264.1 LytTR family DNA-binding domain-containing protein -

Sequence

Protein


Download         Length: 383 a.a.        Molecular weight: 42596.84 Da        Isoelectric Point: 6.3741

>NTDB_id=780972 PIB32_RS14305 WP_308364257.1 3424478..3425629(+) (pilU) [Microbulbifer sp. RZ01]
MEIERLLQLVVEKGASDLFITAGVPPSIKVHGKVVPASSSSLTPEKARELVVGIMNERQRKEFAEKKELNFAIAVRNVGR
FRVSAFFQRNLVGMVLRRIETKIPTIDGLGLPEILKELSMTKRGLIIFVGATGTGKSTSLASMIGHRNENSKGHIISIED
PIEFVHQHRGCIVTQREVGLDTESFEVALKNTLRQAPDVILIGEVRTRETMDHAIAFAETGHLCLCTLHANNANQALDRI
IHFFPADRHSQLWMDLSLNLKAMVAQQLVPTPDGDGRRACLEIMINTPLASDLIRKGEVHKLKELMKRSNEQGMQTFDQA
LYELYDRGEITYEDALAHADSANDLRLMIKLKSESDAEYLNSAAGELSLQNDSEYGDGGPQLY

Nucleotide


Download         Length: 1152 bp        

>NTDB_id=780972 PIB32_RS14305 WP_308364257.1 3424478..3425629(+) (pilU) [Microbulbifer sp. RZ01]
ATGGAAATAGAAAGACTGTTACAACTGGTCGTAGAGAAAGGTGCATCCGACCTGTTTATCACCGCCGGCGTGCCACCATC
CATCAAGGTGCACGGCAAAGTCGTGCCCGCCAGCAGCTCCTCCCTCACCCCGGAAAAGGCCCGCGAACTGGTGGTGGGCA
TCATGAACGAGCGACAGCGCAAGGAATTCGCGGAGAAAAAGGAACTCAACTTCGCCATAGCTGTGCGCAACGTGGGCCGT
TTCCGGGTCTCCGCGTTTTTCCAGCGCAACCTGGTGGGCATGGTGCTGCGCCGCATCGAGACCAAGATTCCCACCATCGA
CGGCCTGGGCCTTCCGGAAATTCTCAAAGAGTTGTCGATGACCAAGCGCGGGCTGATCATCTTCGTGGGCGCCACCGGTA
CCGGTAAATCCACCTCGCTCGCCTCCATGATCGGCCACCGCAATGAAAATTCTAAAGGGCATATCATTTCCATCGAGGAC
CCGATTGAATTCGTGCACCAGCACCGCGGCTGCATTGTCACCCAGCGCGAGGTGGGACTGGATACCGAGTCCTTCGAGGT
CGCTCTGAAGAACACCCTGCGCCAGGCTCCGGACGTGATCCTGATCGGCGAGGTGCGCACCCGCGAGACCATGGACCACG
CCATCGCCTTCGCGGAAACCGGCCACCTGTGCCTGTGCACCCTGCACGCCAACAACGCCAACCAGGCCCTGGACCGCATC
ATCCACTTCTTCCCCGCTGACAGGCACAGCCAGCTGTGGATGGATTTGTCGCTCAACCTCAAAGCCATGGTCGCTCAGCA
GCTGGTGCCCACCCCCGATGGCGACGGGCGCCGCGCCTGCCTGGAAATCATGATCAACACCCCGCTGGCCTCCGACCTGA
TCCGCAAGGGCGAGGTGCACAAGCTGAAGGAGCTGATGAAACGCTCCAACGAGCAGGGCATGCAGACCTTCGACCAGGCC
CTGTACGAACTCTACGATCGCGGCGAGATCACCTACGAAGACGCCCTCGCCCACGCCGACTCCGCCAACGACCTGCGCCT
GATGATCAAGCTCAAGTCCGAATCCGACGCCGAGTACCTGAACAGCGCGGCGGGAGAGCTGAGCCTGCAGAATGATTCGG
AGTATGGAGATGGCGGGCCGCAGCTGTACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(105-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

76.486

96.606

0.739
  pilU Acinetobacter baylyi ADP1

66.387

93.211

0.619
  pilU Vibrio cholerae strain A1552

55.795

96.867

0.54
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.838

88.512

0.397
  pilT Acinetobacter baumannii strain A118

43.284

87.467

0.379
  pilT Acinetobacter nosocomialis M2

43.284

87.467

0.379
  pilT Legionella pneumophila strain ERS1305867

43.284

87.467

0.379
  pilT Legionella pneumophila strain Lp02

43.284

87.467

0.379
  pilT Acinetobacter baumannii D1279779

43.284

87.467

0.379
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.515

87.206

0.371
  pilT Vibrio cholerae strain A1552

42.515

87.206

0.371
  pilT Pseudomonas aeruginosa PAK

42.388

87.467

0.371
  pilT Acinetobacter baylyi ADP1

41.056

89.034

0.366
  pilT Pseudomonas stutzeri DSM 10701

41.493

87.467

0.363