Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PIB32_RS14300 Genome accession   NZ_CP116898
Coordinates   3423333..3424367 (+) Length   344 a.a.
NCBI ID   WP_183459060.1    Uniprot ID   -
Organism   Microbulbifer sp. RZ01     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3418333..3429367
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PIB32_RS14280 - 3418852..3420819 (-) 1968 WP_308364253.1 dynamin family protein -
  PIB32_RS14285 - 3420957..3421565 (-) 609 WP_308364254.1 YggT family protein -
  PIB32_RS14290 proC 3421719..3422546 (-) 828 WP_308364255.1 pyrroline-5-carboxylate reductase -
  PIB32_RS14295 - 3422539..3423246 (-) 708 WP_308364256.1 YggS family pyridoxal phosphate-dependent enzyme -
  PIB32_RS14300 pilT 3423333..3424367 (+) 1035 WP_183459060.1 type IV pilus twitching motility protein PilT Machinery gene
  PIB32_RS14305 pilU 3424478..3425629 (+) 1152 WP_308364257.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PIB32_RS14310 - 3425878..3426432 (+) 555 WP_308375574.1 hypothetical protein -
  PIB32_RS14315 - 3426495..3427244 (+) 750 WP_308364260.1 SDR family oxidoreductase -
  PIB32_RS14320 - 3427277..3427891 (+) 615 WP_308364261.1 LysE family translocator -
  PIB32_RS14325 - 3428021..3428839 (+) 819 WP_308364263.1 hypothetical protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38382.98 Da        Isoelectric Point: 6.5784

>NTDB_id=780971 PIB32_RS14300 WP_183459060.1 3423333..3424367(+) (pilT) [Microbulbifer sp. RZ01]
MDITELLAFSAKQNASDLHLSAGLPPMIRVDGDVRRINLPSMEHKQVHALIYEIMNDKQRKDYEEFLETDFSFEVPGVAR
FRVNAFNHNRGAGAVFRTIPSKVLTMDDLGMGQVFRQISDTPRGLVLVTGPTGSGKSTSLAAMIDYINDNKYEHILTVED
PIEFVHESKKCLVNQREVHRDTHGFAEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAQEKAMVRSMLSESLQAVISQTLLKKNGGGRVAAHEIMRGTPAIRNLIREDKIAQMYSAIQTGANVGMQTMDQCL
QDLVDRRVIAREVAREKAKMPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=780971 PIB32_RS14300 WP_183459060.1 3423333..3424367(+) (pilT) [Microbulbifer sp. RZ01]
ATGGATATCACAGAACTCCTCGCCTTCAGCGCCAAGCAGAACGCCTCCGACTTGCACCTCTCCGCCGGCTTGCCGCCGAT
GATCCGCGTGGACGGCGATGTGCGTCGCATCAACCTGCCGTCCATGGAACACAAGCAGGTGCACGCGCTGATCTACGAGA
TCATGAACGACAAGCAGCGCAAGGATTACGAGGAGTTTCTGGAGACCGACTTCTCCTTCGAGGTGCCCGGCGTGGCGCGC
TTCCGGGTGAACGCCTTCAACCACAATCGCGGCGCCGGCGCGGTGTTCCGGACCATTCCCTCCAAGGTATTGACCATGGA
CGACCTGGGCATGGGCCAGGTGTTCCGCCAGATCTCCGACACCCCCCGCGGCCTGGTGCTGGTCACCGGCCCCACCGGTT
CCGGCAAATCCACCAGCCTGGCGGCGATGATCGACTACATCAACGACAACAAGTACGAACACATCCTCACCGTCGAGGAC
CCGATCGAATTCGTGCACGAATCCAAGAAATGCCTGGTGAACCAGCGCGAAGTGCACCGCGACACCCACGGCTTCGCCGA
GGCGTTGCGCTCGGCCCTGCGGGAAGACCCGGACATCATCCTGGTGGGCGAGATGCGCGACCTGGAAACCATCCGCCTGG
CCCTCACCGCGGCGGAAACCGGCCACCTGGTGTTCGGCACCCTGCACACCACTTCCGCCGCCAAGACCATCGACCGGGTG
GTGGACGTGTTCCCCGCGCAGGAAAAGGCGATGGTGCGCTCCATGCTCTCGGAATCCCTGCAGGCGGTGATCTCCCAGAC
CCTGCTCAAGAAAAACGGCGGCGGCCGCGTGGCAGCCCACGAAATCATGCGCGGTACTCCGGCGATCCGAAACCTTATCC
GCGAGGACAAGATCGCGCAGATGTACTCCGCCATCCAGACCGGCGCCAACGTGGGTATGCAGACCATGGACCAGTGCCTG
CAGGACCTGGTGGATCGGCGTGTAATCGCCCGCGAAGTGGCGCGTGAAAAAGCCAAGATGCCGGAGAACTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

87.791

100

0.878
  pilT Pseudomonas stutzeri DSM 10701

87.209

100

0.872
  pilT Acinetobacter nosocomialis M2

84.012

100

0.84
  pilT Acinetobacter baumannii D1279779

84.012

100

0.84
  pilT Acinetobacter baumannii strain A118

84.012

100

0.84
  pilT Acinetobacter baylyi ADP1

79.942

100

0.799
  pilT Legionella pneumophila strain Lp02

74.26

98.256

0.73
  pilT Legionella pneumophila strain ERS1305867

74.26

98.256

0.73
  pilT Neisseria meningitidis 8013

68.116

100

0.683
  pilT Neisseria gonorrhoeae MS11

67.536

100

0.677
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.461

97.093

0.674
  pilT Vibrio cholerae strain A1552

69.461

97.093

0.674
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

100

0.515
  pilU Acinetobacter baylyi ADP1

39.017

100

0.392
  pilU Vibrio cholerae strain A1552

40.923

94.477

0.387
  pilU Pseudomonas stutzeri DSM 10701

40.432

94.186

0.381