Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PH545_RS00925 Genome accession   NZ_CP116414
Coordinates   172762..173802 (+) Length   346 a.a.
NCBI ID   WP_289880781.1    Uniprot ID   -
Organism   Vibrio harveyi strain K2014767     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 167762..178802
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PH545_RS00890 (PH545_00890) hemW 167781..168953 (-) 1173 WP_274878518.1 radical SAM family heme chaperone HemW -
  PH545_RS00895 (PH545_00895) - 168972..169574 (-) 603 WP_017189094.1 XTP/dITP diphosphatase -
  PH545_RS00900 (PH545_00900) - 169680..170111 (-) 432 WP_005425804.1 DUF4426 domain-containing protein -
  PH545_RS00905 (PH545_00905) yggU 170172..170462 (-) 291 WP_005425805.1 DUF167 family protein YggU -
  PH545_RS00910 (PH545_00910) - 170462..171019 (-) 558 WP_005425806.1 YggT family protein -
  PH545_RS00915 (PH545_00915) proC 171073..171891 (-) 819 WP_038899445.1 pyrroline-5-carboxylate reductase -
  PH545_RS00920 (PH545_00920) - 172023..172733 (-) 711 WP_289880779.1 YggS family pyridoxal phosphate-dependent enzyme -
  PH545_RS00925 (PH545_00925) pilT 172762..173802 (+) 1041 WP_289880781.1 type IV pilus twitching motility protein PilT Machinery gene
  PH545_RS00930 (PH545_00930) pilU 173834..174940 (+) 1107 WP_005449736.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PH545_RS00935 (PH545_00935) ruvX 175000..175425 (-) 426 WP_289880783.1 Holliday junction resolvase RuvX -
  PH545_RS00940 (PH545_00940) - 175475..176038 (-) 564 WP_005449740.1 YqgE/AlgH family protein -
  PH545_RS00945 (PH545_00945) gshB 176168..177118 (-) 951 WP_005449741.1 glutathione synthase -
  PH545_RS00950 (PH545_00950) rsmE 177133..177864 (-) 732 WP_289880784.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  PH545_RS00955 (PH545_00955) endA 178011..178706 (-) 696 WP_289880786.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38676.20 Da        Isoelectric Point: 6.5043

>NTDB_id=777268 PH545_RS00925 WP_289880781.1 172762..173802(+) (pilT) [Vibrio harveyi strain K2014767]
MDITELLDFSVKHNASDLHLSAGVPPMVRIDGDVRKLGIPAFTHQEVHRLVFEIMNDAQRSEFEEKLEVDFSFELHNVGR
FRVNAFNQSRGCAAVFRTIPSSIPTLEELEAPEIFRKIANAEKVLVLVTGPTGSGKSTTLAAIVDYINRNHNKHVLTIED
PIEFVHTNNKCLINQREVHRDTHSFQNALRSALREDPDVILVGEMRDKETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRSVIAQKLLKRNGGGRIACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
RQLMAQGMVSREEVDKKVEIEAQQFS

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=777268 PH545_RS00925 WP_289880781.1 172762..173802(+) (pilT) [Vibrio harveyi strain K2014767]
ATGGATATCACTGAGTTACTGGATTTTAGTGTAAAACATAATGCTTCGGATCTACATCTTTCTGCAGGTGTACCACCAAT
GGTTCGTATTGATGGTGATGTAAGAAAGCTGGGCATTCCAGCGTTTACACACCAAGAAGTGCATCGTTTGGTTTTTGAAA
TCATGAACGATGCGCAGCGCAGTGAGTTCGAAGAAAAACTCGAAGTCGATTTTTCATTTGAATTGCATAACGTTGGCCGT
TTTCGTGTCAACGCTTTCAACCAATCTCGCGGTTGTGCGGCTGTGTTCCGTACCATCCCAAGTAGCATTCCAACACTGGA
AGAGCTAGAAGCCCCAGAGATCTTCAGAAAGATTGCCAATGCTGAAAAAGTACTGGTATTGGTTACCGGTCCAACGGGTT
CTGGTAAATCGACTACCTTGGCAGCCATTGTTGACTACATTAACCGTAATCACAATAAGCACGTCCTAACAATTGAAGAT
CCGATTGAATTTGTTCACACCAACAATAAGTGTCTGATCAACCAACGTGAAGTACACCGTGATACACACAGCTTCCAAAA
CGCACTGCGCAGCGCATTACGTGAAGACCCGGATGTGATTCTAGTTGGTGAGATGCGTGATAAAGAAACCATCAGTTTGG
CGCTGACGGCTGCTGAAACGGGTCACCTTGTTTTCGGTACGCTGCACACTAGCTCAGCAGCGAAAACCATCGACCGTATT
ATCGACGTATTCCCAGGTAGTGACAAAGACATGGTGCGTTCGATGCTGTCGGAGTCGCTGCGTTCGGTTATTGCACAAAA
ACTGTTAAAACGTAATGGTGGTGGTCGTATTGCTTGTCATGAAATCATGATGGCGACACCTGCGATCCGTAACCTTATTC
GTGAAGACAAAGTCGCGCAGATGTATTCGATCATTCAGACCGGTGCGGCGCATGGCATGCAGACCATGGAGCAGAATGCT
CGTCAATTGATGGCACAAGGCATGGTATCGCGTGAGGAAGTGGATAAGAAAGTTGAAATTGAAGCGCAGCAATTCTCGTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

86.087

99.711

0.858
  pilT Vibrio cholerae strain A1552

86.087

99.711

0.858
  pilT Acinetobacter baumannii D1279779

71.818

95.376

0.685
  pilT Acinetobacter baumannii strain A118

71.818

95.376

0.685
  pilT Acinetobacter nosocomialis M2

71.515

95.376

0.682
  pilT Acinetobacter baylyi ADP1

71.212

95.376

0.679
  pilT Pseudomonas stutzeri DSM 10701

68.732

97.977

0.673
  pilT Pseudomonas aeruginosa PAK

68.529

98.266

0.673
  pilT Legionella pneumophila strain Lp02

67.372

95.665

0.645
  pilT Legionella pneumophila strain ERS1305867

67.372

95.665

0.645
  pilT Neisseria meningitidis 8013

63.824

98.266

0.627
  pilT Neisseria gonorrhoeae MS11

63.529

98.266

0.624
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.57

100

0.5
  pilU Vibrio cholerae strain A1552

41.317

96.532

0.399
  pilU Pseudomonas stutzeri DSM 10701

40.12

96.532

0.387