Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PE051_RS19730 Genome accession   NZ_CP115980
Coordinates   4093024..4094004 (-) Length   326 a.a.
NCBI ID   WP_334753724.1    Uniprot ID   -
Organism   Enterobacter asburiae strain WUM-UW/843     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4088024..4099004
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PE051_RS19700 (PE051_19660) - 4088900..4089397 (+) 498 WP_039262968.1 SprT family zinc-dependent metalloprotease -
  PE051_RS19705 (PE051_19665) endA 4089492..4090199 (+) 708 WP_023333343.1 deoxyribonuclease I -
  PE051_RS19710 (PE051_19670) rsmE 4090251..4090982 (+) 732 WP_023309126.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  PE051_RS19715 (PE051_19675) gshB 4091002..4091949 (+) 948 WP_029739222.1 glutathione synthase -
  PE051_RS19720 (PE051_19680) - 4092037..4092597 (+) 561 WP_008499752.1 YqgE/AlgH family protein -
  PE051_RS19725 (PE051_19685) ruvX 4092597..4093013 (+) 417 WP_029739223.1 Holliday junction resolvase RuvX -
  PE051_RS19730 (PE051_19690) pilT 4093024..4094004 (-) 981 WP_334753724.1 type IV pilus twitching motility protein PilT Machinery gene
  PE051_RS19735 (PE051_19695) - 4094022..4094723 (+) 702 WP_039262966.1 YggS family pyridoxal phosphate-dependent enzyme -
  PE051_RS19740 (PE051_19700) - 4094745..4095311 (+) 567 WP_039262965.1 YggT family protein -
  PE051_RS19745 (PE051_19705) yggU 4095308..4095604 (+) 297 WP_023309131.1 DUF167 family protein YggU -
  PE051_RS19750 (PE051_19710) - 4095608..4096201 (+) 594 WP_048979374.1 XTP/dITP diphosphatase -
  PE051_RS19755 (PE051_19715) hemW 4096194..4097336 (+) 1143 WP_045888333.1 radical SAM family heme chaperone HemW -
  PE051_RS19760 (PE051_19720) - 4097400..4097738 (+) 339 WP_039262962.1 endonuclease domain-containing protein -
  PE051_RS19765 (PE051_19725) - 4097821..4098537 (-) 717 WP_033146612.1 DUF2884 domain-containing protein -
  PE051_RS19770 (PE051_19730) - 4098594..4098920 (-) 327 WP_014885198.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35440.47 Da        Isoelectric Point: 6.6934

>NTDB_id=774761 PE051_RS19730 WP_334753724.1 4093024..4094004(-) (pilT) [Enterobacter asburiae strain WUM-UW/843]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRLGRLEPAPFQPPDVGALLKAWLNDEQQGTWWAKGQVDFAATVTGGQRLRG
SAFKQMRGVSVTLRLLPRSCPQLASLGAPRVIPELLSNDAGLILVTGAAGSGKSTTLAAMVDFLNHHADGHILTLEDPVE
FIYQSERCLIQQREIGQHSPSFAEALRSALRQDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGASQAIERLVDT
FPAQEKDPVRSQLAGSLRAVLAQRLLPDLQGGCVALYELLVNTAAAANLIREGKTWQLPGIIQTGQQAGMQNFDQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=774761 PE051_RS19730 WP_334753724.1 4093024..4094004(-) (pilT) [Enterobacter asburiae strain WUM-UW/843]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCGCG
CTGGCGCAGATTAGGCCGGCTTGAACCCGCTCCCTTTCAGCCTCCCGACGTTGGGGCGTTATTAAAAGCGTGGCTTAACG
ATGAGCAGCAGGGAACATGGTGGGCAAAGGGGCAGGTGGATTTTGCCGCAACGGTAACGGGAGGCCAGCGCCTGCGCGGC
AGTGCCTTTAAGCAGATGAGAGGTGTCTCTGTGACGCTGCGGCTGTTGCCGCGTAGCTGCCCGCAGCTCGCTTCGCTGGG
CGCGCCGCGGGTAATCCCGGAACTGTTATCCAATGACGCCGGGCTGATTCTGGTCACGGGGGCGGCCGGCAGCGGCAAAT
CCACTACGCTGGCGGCGATGGTCGATTTTCTAAACCACCATGCTGACGGCCATATCCTCACGCTTGAAGATCCGGTGGAG
TTTATCTACCAGAGCGAACGTTGCCTGATCCAGCAGCGGGAGATAGGCCAGCACAGCCCGTCATTTGCCGAAGCGCTGCG
CAGTGCCTTGCGCCAGGATCCGGACGTTATTTTGCTGGGGGAGCTGCGCGACAGCGAAACGATCCGCCTGGCGCTGACGG
CGGCGGAAACCGGCCATCTGGTGCTGGCGACGCTGCATACGCGCGGGGCATCGCAGGCGATTGAACGGCTGGTCGATACG
TTCCCGGCGCAGGAGAAAGATCCGGTGCGTAGCCAGCTGGCCGGTAGCCTGCGGGCGGTGCTGGCGCAGAGGCTACTTCC
CGATCTACAGGGCGGGTGCGTCGCGTTGTATGAACTGCTGGTGAACACTGCGGCGGCGGCGAATTTGATTCGTGAAGGGA
AAACGTGGCAACTGCCCGGGATCATTCAAACCGGTCAGCAGGCAGGAATGCAGAACTTTGACCAGAGCCTGGCGGAGAGA
CGGGCGCAGGGGCGGCTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(88-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.153

100

0.503
  pilT Vibrio cholerae strain A1552

50.153

100

0.503
  pilT Pseudomonas stutzeri DSM 10701

48.318

100

0.485
  pilT Legionella pneumophila strain ERS1305867

48.447

98.773

0.479
  pilT Legionella pneumophila strain Lp02

48.447

98.773

0.479
  pilT Acinetobacter baumannii D1279779

47.706

100

0.479
  pilT Acinetobacter baumannii strain A118

47.706

100

0.479
  pilT Pseudomonas aeruginosa PAK

47.706

100

0.479
  pilT Acinetobacter baylyi ADP1

47.401

100

0.475
  pilT Neisseria meningitidis 8013

47.256

100

0.475
  pilT Acinetobacter nosocomialis M2

48.125

98.16

0.472
  pilT Neisseria gonorrhoeae MS11

46.951

100

0.472
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.168

98.773

0.426
  pilU Pseudomonas stutzeri DSM 10701

39.091

100

0.396
  pilU Vibrio cholerae strain A1552

39.13

98.773

0.386