Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PG903_RS12530 Genome accession   NZ_CP115952
Coordinates   2613029..2614168 (-) Length   379 a.a.
NCBI ID   WP_003271877.1    Uniprot ID   A0AAP7ZNZ2
Organism   Ralstonia solanacearum CFBP2957     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2608029..2619168
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PG903_RS12505 - 2609743..2610279 (-) 537 WP_013205204.1 PilX N-terminal domain-containing pilus assembly protein -
  PG903_RS12510 - 2610283..2611317 (-) 1035 WP_013205203.1 PilW family protein -
  PG903_RS12515 pilV 2611314..2611904 (-) 591 WP_013205202.1 type IV pilus modification protein PilV -
  PG903_RS12520 - 2611901..2612389 (-) 489 WP_013205201.1 GspH/FimT family pseudopilin -
  PG903_RS12525 - 2612393..2612833 (-) 441 WP_043908211.1 type IV pilin protein -
  PG903_RS12530 pilU 2613029..2614168 (-) 1140 WP_003271877.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PG903_RS12535 pilT 2614218..2615261 (-) 1044 WP_003263111.1 type IV pilus twitching motility protein PilT Machinery gene
  PG903_RS12540 - 2615383..2616105 (+) 723 WP_013205198.1 YggS family pyridoxal phosphate-dependent enzyme -
  PG903_RS12545 proC 2616140..2616964 (+) 825 WP_013205197.1 pyrroline-5-carboxylate reductase -
  PG903_RS12550 - 2616973..2617251 (+) 279 WP_013205196.1 hypothetical protein -
  PG903_RS12555 ubiA 2617248..2618123 (-) 876 WP_013205195.1 4-hydroxybenzoate octaprenyltransferase -
  PG903_RS12560 - 2618197..2618697 (-) 501 WP_003271865.1 Dps family protein -
  PG903_RS12565 - 2618856..2619098 (-) 243 WP_003263116.1 hypothetical protein -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42235.66 Da        Isoelectric Point: 6.9314

>NTDB_id=774585 PG903_RS12530 WP_003271877.1 2613029..2614168(-) (pilU) [Ralstonia solanacearum CFBP2957]
MLDRDAATKYIHELLQLMVNSRGSDLFITAEFPPAIKVDGKVTPISQQPLTTVQAMGLVKAIMNEKQLREYEDSFECNFA
ITAPTAGRFRVSAFMQQGRAGMVLRTINTKIPTLTELDLPPILNEVAMSKRGLVVVVGATGSGKSTTLAAMVGYRNAHSY
GHIITIEDPVEYVHAHHNCVVTQREVGVDTESWHVALKNTLRQAPDVILIGEIRDRDTMEYAIQYAETGHLCLATLHANS
SNQAIDRIVNFFPEERRQQLLIDLSLNLRAMIAQRLLPRKGRKGRAPAIEILLGTPLVSDLIFKGEVHELKEVMKKSREQ
GMVTFDQALFELYEADKITYEDALRNADSLNDLRLQIKLHGKRGGPADLTAGTEHLNVM

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=774585 PG903_RS12530 WP_003271877.1 2613029..2614168(-) (pilU) [Ralstonia solanacearum CFBP2957]
ATGCTGGACCGCGACGCCGCCACCAAATACATCCACGAACTCTTGCAGCTCATGGTGAACAGCCGTGGCTCGGACCTGTT
CATCACCGCTGAATTCCCGCCCGCCATCAAGGTGGACGGCAAAGTCACGCCGATCTCGCAGCAGCCGTTGACCACCGTCC
AGGCGATGGGCCTGGTCAAGGCGATCATGAACGAGAAGCAACTGCGCGAGTACGAAGACTCGTTCGAGTGCAACTTCGCC
ATCACCGCGCCCACCGCCGGCCGCTTCCGCGTGTCGGCGTTCATGCAGCAGGGCCGCGCCGGCATGGTGCTGCGGACCAT
CAACACCAAGATCCCGACGCTCACCGAACTGGACCTGCCGCCCATCCTCAACGAAGTCGCGATGAGCAAGCGCGGGCTCG
TGGTCGTGGTGGGGGCGACGGGTTCGGGCAAGTCGACCACGCTGGCGGCGATGGTCGGCTACCGCAACGCGCATTCGTAC
GGCCACATCATCACCATCGAAGACCCGGTGGAATACGTGCACGCACACCACAACTGCGTCGTGACGCAGCGCGAGGTGGG
CGTGGATACCGAGTCGTGGCACGTGGCGCTGAAGAACACGCTGCGCCAGGCGCCGGACGTGATCCTGATCGGCGAAATCC
GCGATCGCGACACGATGGAATACGCCATCCAGTACGCCGAAACCGGCCACTTGTGCCTGGCCACGCTGCACGCCAACAGC
TCGAACCAGGCGATCGACCGGATCGTCAACTTCTTCCCGGAAGAGCGGCGTCAGCAACTGCTGATCGATCTGTCCCTGAA
CCTGCGCGCGATGATCGCGCAGCGCCTGCTGCCGCGCAAAGGCAGGAAGGGCCGTGCGCCGGCGATCGAAATCCTGCTGG
GTACGCCGCTGGTGTCCGACCTGATCTTCAAGGGCGAGGTGCACGAGCTCAAGGAGGTCATGAAGAAGTCTCGCGAGCAG
GGGATGGTCACGTTCGACCAGGCACTGTTCGAGCTCTATGAGGCGGACAAGATCACCTATGAAGATGCGCTGCGCAACGC
GGATTCGCTGAACGACCTGCGTTTGCAGATCAAGCTGCACGGCAAGCGCGGCGGGCCGGCGGATCTGACGGCCGGCACCG
AGCATCTCAACGTGATGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(61-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.756

93.14

0.575
  pilU Acinetobacter baylyi ADP1

54.875

94.723

0.52
  pilU Vibrio cholerae strain A1552

55.367

93.404

0.517
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.917

88.918

0.391
  pilT Legionella pneumophila strain Lp02

43.544

87.863

0.383
  pilT Legionella pneumophila strain ERS1305867

43.544

87.863

0.383
  pilT Pseudomonas aeruginosa PAK

43.027

88.918

0.383
  pilT Vibrio cholerae strain A1552

45.741

83.641

0.383
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

45.741

83.641

0.383
  pilT Acinetobacter baylyi ADP1

44.72

84.96

0.38
  pilT Pseudomonas stutzeri DSM 10701

41.84

88.918

0.372
  pilT Acinetobacter baumannii D1279779

43.887

84.169

0.369
  pilT Acinetobacter baumannii strain A118

43.887

84.169

0.369
  pilT Acinetobacter nosocomialis M2

43.887

84.169

0.369