Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PG903_RS12535 Genome accession   NZ_CP115952
Coordinates   2614218..2615261 (-) Length   347 a.a.
NCBI ID   WP_003263111.1    Uniprot ID   -
Organism   Ralstonia solanacearum CFBP2957     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2609218..2620261
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PG903_RS12505 - 2609743..2610279 (-) 537 WP_013205204.1 PilX N-terminal domain-containing pilus assembly protein -
  PG903_RS12510 - 2610283..2611317 (-) 1035 WP_013205203.1 PilW family protein -
  PG903_RS12515 pilV 2611314..2611904 (-) 591 WP_013205202.1 type IV pilus modification protein PilV -
  PG903_RS12520 - 2611901..2612389 (-) 489 WP_013205201.1 GspH/FimT family pseudopilin -
  PG903_RS12525 - 2612393..2612833 (-) 441 WP_043908211.1 type IV pilin protein -
  PG903_RS12530 pilU 2613029..2614168 (-) 1140 WP_003271877.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PG903_RS12535 pilT 2614218..2615261 (-) 1044 WP_003263111.1 type IV pilus twitching motility protein PilT Machinery gene
  PG903_RS12540 - 2615383..2616105 (+) 723 WP_013205198.1 YggS family pyridoxal phosphate-dependent enzyme -
  PG903_RS12545 proC 2616140..2616964 (+) 825 WP_013205197.1 pyrroline-5-carboxylate reductase -
  PG903_RS12550 - 2616973..2617251 (+) 279 WP_013205196.1 hypothetical protein -
  PG903_RS12555 ubiA 2617248..2618123 (-) 876 WP_013205195.1 4-hydroxybenzoate octaprenyltransferase -
  PG903_RS12560 - 2618197..2618697 (-) 501 WP_003271865.1 Dps family protein -
  PG903_RS12565 - 2618856..2619098 (-) 243 WP_003263116.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38166.80 Da        Isoelectric Point: 6.8705

>NTDB_id=774586 PG903_RS12535 WP_003263111.1 2614218..2615261(-) (pilT) [Ralstonia solanacearum CFBP2957]
MDIAQLLAFAAKNKASDLHLSAGLPPMIRIHGDMRRINVPPLTHQDVHAMVYDIMSDVQRKHYEENLEADFSFEIPGLSR
FRVNAFNQNRGAAAVFRTIPSKVLTLEDLKAPAVFSDLAMKPRGLVLVTGPTGSGKSTTLAAMVNHRNESDLGHILTVED
PIEFVHESKKSLINQRELGPHTHSFANALKSALREDPDVVLVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRV
VDVFPSDEKDMVRTMLSESLEAVISQTLLKTRDGSGRVAAHEIMICTPAIRHLIRENKISQMYSMMQTSSGLGMQTLDQC
LAELIKRSAINYADARAIAKNPDAFAN

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=774586 PG903_RS12535 WP_003263111.1 2614218..2615261(-) (pilT) [Ralstonia solanacearum CFBP2957]
ATGGACATCGCGCAGCTTCTGGCCTTCGCCGCCAAGAACAAAGCGTCTGATCTGCACTTGTCGGCGGGCTTGCCACCGAT
GATCCGGATCCATGGCGACATGCGTCGCATCAACGTGCCGCCGCTCACGCACCAGGATGTCCACGCCATGGTGTACGACA
TCATGAGTGACGTGCAGCGCAAGCATTACGAAGAAAACCTGGAAGCGGACTTCTCGTTCGAGATTCCCGGGTTGTCGCGT
TTCCGGGTCAACGCCTTCAATCAGAACCGCGGCGCCGCCGCCGTGTTCCGGACGATTCCGTCCAAGGTCCTGACGCTGGA
AGACCTGAAGGCGCCCGCCGTCTTCTCCGACCTCGCCATGAAGCCGCGCGGCCTGGTGCTGGTGACGGGCCCGACCGGTT
CGGGCAAGTCGACCACGCTGGCGGCGATGGTCAACCACCGCAACGAAAGCGATCTGGGCCACATCCTCACGGTGGAGGAC
CCGATCGAATTCGTGCACGAATCCAAGAAGAGCCTGATCAACCAGCGTGAGCTGGGGCCGCATACGCACTCGTTCGCCAA
CGCGCTGAAATCGGCGCTGCGGGAAGACCCGGACGTGGTCCTGGTCGGCGAATTGCGGGACCTGGAAACCATCCGCCTGG
CACTGACGGCGGCCGAAACCGGCCACTTGGTGTTTGGCACGCTGCACACGAGTTCCGCGGCCAAGACCATCGACCGGGTG
GTCGACGTGTTCCCCTCGGATGAGAAGGACATGGTCCGCACCATGCTGTCCGAATCGCTGGAAGCGGTGATCTCGCAGAC
GTTGCTCAAGACGCGCGACGGCTCCGGCCGGGTCGCGGCGCACGAGATCATGATCTGCACGCCGGCCATCCGACACCTGA
TCCGCGAGAACAAGATTTCGCAGATGTATTCGATGATGCAGACCAGCAGCGGGCTGGGCATGCAGACGCTGGACCAATGC
CTGGCGGAGCTCATCAAGCGCTCGGCGATCAACTACGCCGACGCGCGCGCCATCGCCAAGAACCCGGACGCGTTCGCGAA
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

71.594

99.424

0.712
  pilT Acinetobacter baumannii D1279779

71.594

99.424

0.712
  pilT Acinetobacter baumannii strain A118

71.594

99.424

0.712
  pilT Pseudomonas aeruginosa PAK

71.304

99.424

0.709
  pilT Pseudomonas stutzeri DSM 10701

71.014

99.424

0.706
  pilT Acinetobacter baylyi ADP1

69.855

99.424

0.695
  pilT Neisseria gonorrhoeae MS11

68.497

99.712

0.683
  pilT Neisseria meningitidis 8013

68.497

99.712

0.683
  pilT Legionella pneumophila strain Lp02

67.826

99.424

0.674
  pilT Legionella pneumophila strain ERS1305867

67.826

99.424

0.674
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.469

97.118

0.646
  pilT Vibrio cholerae strain A1552

66.469

97.118

0.646
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.867

99.712

0.507
  pilU Acinetobacter baylyi ADP1

42.442

99.135

0.421
  pilU Pseudomonas stutzeri DSM 10701

42.388

96.542

0.409
  pilU Vibrio cholerae strain A1552

41.791

96.542

0.403