Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   O8I58_RS06505 Genome accession   NZ_CP114978
Coordinates   1479315..1479758 (+) Length   147 a.a.
NCBI ID   WP_298321584.1    Uniprot ID   -
Organism   MAG: Pseudoxanthomonas sp. isolate Chal_Metagen_Bin2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1474315..1484758
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O8I58_RS06495 (O8I58_06495) - 1475285..1476904 (+) 1620 WP_298321582.1 ATP-binding protein -
  O8I58_RS06500 (O8I58_06500) pilR 1477362..1478807 (+) 1446 WP_298321583.1 sigma-54 dependent transcriptional regulator Regulator
  O8I58_RS06505 (O8I58_06505) pilA2 1479315..1479758 (+) 444 WP_298321584.1 pilin Machinery gene
  O8I58_RS06510 (O8I58_06510) pilB 1480008..1481738 (+) 1731 WP_298321585.1 type IV-A pilus assembly ATPase PilB Machinery gene
  O8I58_RS06515 (O8I58_06515) pilC 1481931..1483193 (+) 1263 WP_298321586.1 type II secretion system F family protein Machinery gene
  O8I58_RS06520 (O8I58_06520) - 1483197..1484060 (+) 864 WP_298321587.1 A24 family peptidase -

Sequence

Protein


Download         Length: 147 a.a.        Molecular weight: 15256.48 Da        Isoelectric Point: 4.0813

>NTDB_id=767664 O8I58_RS06505 WP_298321584.1 1479315..1479758(+) (pilA2) [MAG: Pseudoxanthomonas sp. isolate Chal_Metagen_Bin2]
MKKNMQGGFTLIELMIVVAIIAILAAIALPAYQDYTIRSKVSEALVMADAAKLAVTETAASQGVVATEVADNDAAGYTSN
ETKYVASIEIEDGVITVTTQDTGATTAPVLLMTPTQATTDVPVQWVCSLSAGDPKYVPASCRTAATP

Nucleotide


Download         Length: 444 bp        

>NTDB_id=767664 O8I58_RS06505 WP_298321584.1 1479315..1479758(+) (pilA2) [MAG: Pseudoxanthomonas sp. isolate Chal_Metagen_Bin2]
ATGAAGAAGAACATGCAGGGCGGTTTCACCCTCATCGAACTGATGATCGTGGTCGCCATCATCGCCATCCTGGCGGCCAT
CGCGCTGCCGGCGTATCAGGACTACACCATTCGCTCCAAGGTTTCTGAGGCACTGGTGATGGCCGATGCGGCCAAGCTTG
CTGTCACCGAAACTGCTGCAAGCCAAGGGGTCGTAGCTACCGAAGTAGCTGACAATGATGCGGCTGGCTATACCAGCAAT
GAGACCAAGTATGTCGCCTCGATTGAGATCGAGGATGGCGTAATTACCGTCACCACCCAGGATACCGGCGCAACCACCGC
CCCCGTTCTGTTGATGACCCCCACCCAGGCAACCACTGATGTACCAGTCCAGTGGGTTTGCTCACTTTCAGCTGGCGATC
CCAAGTACGTTCCGGCTAGCTGCCGCACTGCTGCGACACCCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(38-142)

Prokaryotic N-terminal methylation motif (N_methyl)

(4-29)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

55.714

95.238

0.531
  pilA2 Legionella pneumophila strain ERS1305867

55

95.238

0.524
  pilA Ralstonia pseudosolanacearum GMI1000

40.805

100

0.483
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

36.364

100

0.463
  comP Acinetobacter baylyi ADP1

44.295

100

0.449
  pilE Neisseria gonorrhoeae strain FA1090

40.373

100

0.442
  pilE Neisseria gonorrhoeae MS11

37.423

100

0.415
  pilA/pilA1 Eikenella corrodens VA1

36.306

100

0.388
  pilA/pilAII Pseudomonas stutzeri DSM 10701

41.912

92.517

0.388
  pilA/pilAI Pseudomonas stutzeri DSM 10701

38.356

99.32

0.381
  pilA Acinetobacter baumannii strain A118

36.242

100

0.367
  pilA Haemophilus influenzae 86-028NP

36.806

97.959

0.361