Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   O8I58_RS06510 Genome accession   NZ_CP114978
Coordinates   1480008..1481738 (+) Length   576 a.a.
NCBI ID   WP_298321585.1    Uniprot ID   -
Organism   MAG: Pseudoxanthomonas sp. isolate Chal_Metagen_Bin2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1475008..1486738
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O8I58_RS06495 (O8I58_06495) - 1475285..1476904 (+) 1620 WP_298321582.1 ATP-binding protein -
  O8I58_RS06500 (O8I58_06500) pilR 1477362..1478807 (+) 1446 WP_298321583.1 sigma-54 dependent transcriptional regulator Regulator
  O8I58_RS06505 (O8I58_06505) pilA2 1479315..1479758 (+) 444 WP_298321584.1 pilin Machinery gene
  O8I58_RS06510 (O8I58_06510) pilB 1480008..1481738 (+) 1731 WP_298321585.1 type IV-A pilus assembly ATPase PilB Machinery gene
  O8I58_RS06515 (O8I58_06515) pilC 1481931..1483193 (+) 1263 WP_298321586.1 type II secretion system F family protein Machinery gene
  O8I58_RS06520 (O8I58_06520) - 1483197..1484060 (+) 864 WP_298321587.1 A24 family peptidase -
  O8I58_RS06525 (O8I58_06525) - 1484156..1485160 (-) 1005 WP_298321588.1 class I fructose-bisphosphate aldolase -

Sequence

Protein


Download         Length: 576 a.a.        Molecular weight: 62849.23 Da        Isoelectric Point: 6.5150

>NTDB_id=767665 O8I58_RS06510 WP_298321585.1 1480008..1481738(+) (pilB) [MAG: Pseudoxanthomonas sp. isolate Chal_Metagen_Bin2]
MNTMAANSANLVGITGIARRLVIDGTMEEAVARTAMAEATQAKIPLAQWIQEKKLVAPAGLAAANAIEFGIPLMDVGVFD
ASQNALKLVSEELLQKHQVLPLFKRGNKLYVGVSDPTRMQALDEIKFHTNLVVEPILVPDDQLKRTLEAWRNASDSLGAL
GGDEEGLDNLDTASGDEDGTDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYETDYRVRLRIDGILKHVAKAPHK
LNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTRQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEPDQQKLF
LEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNTKRGMTFAAALRSFLRQDPDI
IMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCPRCKKPHDIPPH
ALLAEGFTQAELDAGFTLYEAVGCDECTEGYKGRVGIYQVMPMTDEISSIVLEGGGAIQIAEAAQRSNIRDLRQSALWKA
KQGMTSLAEINRVTKD

Nucleotide


Download         Length: 1731 bp        

>NTDB_id=767665 O8I58_RS06510 WP_298321585.1 1480008..1481738(+) (pilB) [MAG: Pseudoxanthomonas sp. isolate Chal_Metagen_Bin2]
ATGAATACGATGGCCGCCAACAGCGCCAATCTTGTCGGCATCACCGGCATTGCCCGTCGCCTGGTCATAGATGGGACCAT
GGAGGAGGCCGTCGCCCGCACGGCCATGGCAGAAGCCACCCAGGCCAAGATCCCGCTGGCCCAGTGGATCCAGGAGAAAA
AATTGGTCGCGCCCGCCGGATTGGCCGCCGCAAATGCCATCGAATTCGGCATACCGTTGATGGATGTTGGCGTCTTCGAT
GCGTCCCAGAACGCGCTCAAGCTGGTCAGCGAAGAGCTACTGCAGAAGCACCAGGTCCTGCCGCTGTTCAAACGCGGCAA
CAAACTCTATGTAGGCGTCTCCGATCCGACCAGAATGCAGGCCCTGGACGAGATTAAATTCCACACCAACCTGGTGGTGG
AGCCGATCCTGGTACCGGACGACCAGCTCAAGCGCACCCTGGAGGCCTGGCGTAATGCCTCCGATTCGCTGGGTGCGCTC
GGCGGGGACGAGGAAGGCCTGGATAACCTCGATACGGCCAGCGGCGACGAGGACGGCACTGACAGCGGCGTGGATGCCAA
GGGCGACGACACCCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGCCGCGGCGCCTCGGACATCCATT
TCGAACCCTACGAAACCGACTACCGTGTGCGCCTGCGCATCGACGGCATCCTGAAACATGTCGCCAAGGCGCCGCACAAA
CTCAACCAGCGCATCGCCGCGCGCCTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAGCGCGTGCCACAGGACGGACG
CATCAAGCTCAACCTGTCCAAGACCCGCCAGATCGACTTCCGCGTCAGCACCCTGCCGACCCTGTTCGGCGAAAAGGTCG
TGCTGCGTATCCTCGATGGCAGCGCCGCCAAACTCGGCATCGACAAACTGGGCTACGAGCCGGACCAGCAGAAACTCTTC
CTGGAGGCCATCCACAAGCCGTACGGCATGGTCCTGGTGACCGGCCCGACCGGCTCGGGCAAGACCGTATCGCTGTATAC
GGCGCTGGGCATTTTGAACGACGAGACCCGCAATATTTCCACCGCCGAGGACCCTGTCGAAATCCGCCTGCCGGGCGTCA
ACCAGGTCCAGCAGAACACCAAGCGCGGCATGACCTTCGCTGCCGCGCTGCGTAGCTTCCTGCGCCAGGACCCGGACATC
ATCATGGTCGGCGAAATTCGCGACCTGGAAACCGCGGAGATCGCGATCAAGGCCGCGCAGACCGGCCACATGGTGCTGTC
CACACTGCACACCAATGACGCACCGCAGACCATCGCACGCCTGATGAACATGGGCATCGCGCCGTACAACATCACCAGCT
CGGTGACGCTGGTGATCGCCCAGCGCCTGGCCCGACGGCTGTGCCCACGCTGCAAGAAGCCGCATGACATTCCGCCGCAC
GCGCTGCTGGCGGAAGGCTTCACCCAGGCGGAACTCGATGCAGGCTTCACCCTCTACGAGGCCGTTGGCTGCGACGAGTG
CACCGAGGGCTACAAGGGGCGTGTCGGCATCTACCAGGTGATGCCGATGACCGATGAGATCAGCAGCATCGTGCTGGAAG
GCGGTGGCGCCATCCAGATTGCCGAGGCCGCCCAGCGCTCAAACATCCGCGACCTGCGCCAGTCGGCGCTTTGGAAAGCC
AAGCAGGGCATGACCAGCCTGGCCGAGATCAACCGCGTCACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(199-466)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(52-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.168

98.09

0.561
  pilB Acinetobacter baylyi ADP1

55.42

99.306

0.55
  pilB Legionella pneumophila strain ERS1305867

52.473

98.264

0.516
  pilB Vibrio cholerae strain A1552

50.967

98.785

0.503
  pilF Neisseria gonorrhoeae MS11

49.819

96.181

0.479
  pilB Vibrio parahaemolyticus RIMD 2210633

50.394

88.194

0.444
  pilB Vibrio campbellii strain DS40M4

49.708

89.063

0.443
  pilF Thermus thermophilus HB27

39.848

91.493

0.365
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.36

92.188

0.363