Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OXH18_RS21290 Genome accession   NZ_CP113797
Coordinates   4946213..4947331 (+) Length   372 a.a.
NCBI ID   WP_268609474.1    Uniprot ID   A0A9E8ZAN2
Organism   Thermocoleostomius sinensis A174     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4941213..4952331
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OXH18_RS21275 (OXH18_21275) - 4942193..4943131 (-) 939 WP_268609471.1 sulfotransferase -
  OXH18_RS21280 (OXH18_21280) - 4943376..4943969 (-) 594 WP_268609472.1 hypothetical protein -
  OXH18_RS21285 (OXH18_21285) pilB/pilB1 4944108..4946105 (+) 1998 WP_268609473.1 GspE/PulE family protein Machinery gene
  OXH18_RS21290 (OXH18_21290) pilT 4946213..4947331 (+) 1119 WP_268609474.1 type IV pilus twitching motility protein PilT Machinery gene
  OXH18_RS21295 (OXH18_21295) - 4947443..4948678 (+) 1236 WP_268609475.1 type II secretion system F family protein -
  OXH18_RS21300 (OXH18_21300) - 4948697..4949284 (+) 588 WP_268609476.1 hypothetical protein -
  OXH18_RS21305 (OXH18_21305) - 4949597..4949866 (-) 270 WP_268609477.1 hypothetical protein -
  OXH18_RS21310 (OXH18_21310) - 4950002..4950346 (+) 345 WP_268609478.1 hypothetical protein -
  OXH18_RS21315 (OXH18_21315) - 4950462..4951673 (-) 1212 WP_268609479.1 hypothetical protein -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 40906.18 Da        Isoelectric Point: 7.1090

>NTDB_id=763117 OXH18_RS21290 WP_268609474.1 4946213..4947331(+) (pilT) [Thermocoleostomius sinensis A174]
MELMIEDLMEQLIEMGGSDLHLSAGLPPYFRISGKLTPIGDEPMTAEQCQRLIFSMLNNTQRKTLEQNWELDCSYGVKGL
ARFRVNVYKDRGTYAACLRALSSKIPNFEKLGLPDIVREMSEKPRGLILVTGPTGSGKTTTLAAMIDLINRTRAEHILTV
EDPIEFVYEPIKSLVHQRQLGEDTKSFANALKAALREDPDIILVGEMRDLETISLAISAAETGHLVFGTLHTSSAAQTVD
RMIDVFPSERQTQVRVQLSNSLVAVFSQTLVPKKNPKPGEYGRVMAQEIMVVTPAISNLIREGKTAQIYSSIQTGGKHGM
QTLEKVLADLYKAGTISFEAAMSKTSKPDEVQRLIGSTPPPAAQPAKAAVRH

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=763117 OXH18_RS21290 WP_268609474.1 4946213..4947331(+) (pilT) [Thermocoleostomius sinensis A174]
ATGGAATTAATGATTGAAGATTTAATGGAACAACTGATTGAAATGGGAGGTTCCGATCTTCATCTATCAGCTGGATTGCC
GCCTTACTTTCGCATTAGTGGTAAATTAACACCGATTGGTGATGAACCCATGACCGCAGAGCAATGTCAGCGGCTAATTT
TCAGTATGTTGAACAACACGCAGCGCAAAACCCTAGAGCAAAATTGGGAATTGGACTGCTCCTATGGCGTCAAGGGCTTA
GCCCGGTTTCGCGTCAATGTCTATAAAGATCGAGGGACATATGCTGCATGTTTACGAGCATTAAGTTCTAAAATTCCTAA
TTTTGAAAAGCTAGGGCTGCCAGATATTGTCCGCGAAATGTCTGAAAAACCTAGAGGTTTGATTTTGGTAACGGGTCCAA
CGGGATCGGGCAAAACCACAACGTTAGCTGCCATGATTGACTTAATCAACCGCACCCGTGCCGAACACATTTTAACGGTG
GAAGATCCGATCGAATTTGTTTATGAACCGATCAAAAGCTTGGTGCACCAGCGACAATTAGGCGAAGACACAAAAAGCTT
TGCCAACGCCCTCAAAGCGGCACTGCGAGAAGACCCAGATATCATTCTGGTGGGTGAAATGCGCGACCTGGAAACAATCT
CTCTGGCAATCTCAGCGGCCGAAACGGGGCACTTGGTATTTGGCACACTCCACACTAGTTCTGCTGCACAAACGGTCGAT
CGCATGATCGATGTATTTCCATCCGAGCGTCAAACCCAAGTCAGGGTGCAGCTATCTAACTCCCTAGTGGCAGTATTCAG
CCAAACGCTCGTACCCAAAAAGAATCCTAAACCGGGTGAATATGGACGAGTCATGGCGCAAGAAATTATGGTCGTGACAC
CTGCTATCTCTAACCTAATTCGCGAAGGCAAAACGGCACAAATCTACTCCTCCATCCAAACGGGCGGCAAGCATGGAATG
CAAACCCTAGAGAAAGTCCTGGCCGATCTCTACAAAGCAGGCACCATCTCTTTTGAAGCCGCAATGTCTAAAACCTCAAA
ACCCGATGAAGTACAGCGCCTAATTGGAAGCACACCGCCGCCTGCTGCCCAACCCGCTAAAGCGGCTGTTCGACACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(44-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

54.783

92.742

0.508
  pilT Pseudomonas stutzeri DSM 10701

54.783

92.742

0.508
  pilT Legionella pneumophila strain ERS1305867

54.493

92.742

0.505
  pilT Acinetobacter baylyi ADP1

54.493

92.742

0.505
  pilT Legionella pneumophila strain Lp02

54.493

92.742

0.505
  pilT Acinetobacter baumannii strain A118

52.754

92.742

0.489
  pilT Acinetobacter nosocomialis M2

52.754

92.742

0.489
  pilT Acinetobacter baumannii D1279779

52.754

92.742

0.489
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.581

100

0.476
  pilT Neisseria gonorrhoeae MS11

51.327

91.129

0.468
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.882

91.398

0.465
  pilT Vibrio cholerae strain A1552

50.882

91.398

0.465
  pilT Neisseria meningitidis 8013

51.032

91.129

0.465
  pilU Pseudomonas stutzeri DSM 10701

38.462

94.355

0.363