Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   OXH18_RS21285 Genome accession   NZ_CP113797
Coordinates   4944108..4946105 (+) Length   665 a.a.
NCBI ID   WP_268609473.1    Uniprot ID   A0A9E8ZAN3
Organism   Thermocoleostomius sinensis A174     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4939108..4951105
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OXH18_RS21265 (OXH18_21265) - 4939149..4939640 (-) 492 WP_268609469.1 pentapeptide repeat-containing protein -
  OXH18_RS21270 (OXH18_21270) - 4939776..4942046 (+) 2271 WP_268609470.1 pentapeptide repeat-containing protein -
  OXH18_RS21275 (OXH18_21275) - 4942193..4943131 (-) 939 WP_268609471.1 sulfotransferase -
  OXH18_RS21280 (OXH18_21280) - 4943376..4943969 (-) 594 WP_268609472.1 hypothetical protein -
  OXH18_RS21285 (OXH18_21285) pilB/pilB1 4944108..4946105 (+) 1998 WP_268609473.1 GspE/PulE family protein Machinery gene
  OXH18_RS21290 (OXH18_21290) pilT 4946213..4947331 (+) 1119 WP_268609474.1 type IV pilus twitching motility protein PilT Machinery gene
  OXH18_RS21295 (OXH18_21295) - 4947443..4948678 (+) 1236 WP_268609475.1 type II secretion system F family protein -
  OXH18_RS21300 (OXH18_21300) - 4948697..4949284 (+) 588 WP_268609476.1 hypothetical protein -
  OXH18_RS21305 (OXH18_21305) - 4949597..4949866 (-) 270 WP_268609477.1 hypothetical protein -
  OXH18_RS21310 (OXH18_21310) - 4950002..4950346 (+) 345 WP_268609478.1 hypothetical protein -

Sequence

Protein


Download         Length: 665 a.a.        Molecular weight: 74178.25 Da        Isoelectric Point: 5.0913

>NTDB_id=763116 OXH18_RS21285 WP_268609473.1 4944108..4946105(+) (pilB/pilB1) [Thermocoleostomius sinensis A174]
MANSSSPRRALVVQQNFSPFSNKLVKSGFVSGEQMQQALIESRKTGRPLTDILAMMTGQQLPLDLLRQYKKQQLFELKIL
YGVESLDPEISQVNSAQMHLLIETLFPIDLCRRYRLIPLSRSETEPPFVLVAMVDPDDLSAQDDLNRILRPQGLGLQRLV
ITAEDYQRIISQYLDEQVEKQKQLEFQAKVDVKSDIENLDYLDLEDTVDDADANLDAALQDPEAAPIVALANKILAKALQ
EGISDIHIEPQEEHLRIRFRKDGVLRLGFELLPKKIIPALTARFKIMANLDIAERRMPQDGRIRRLFDNRKVDFRVNTLP
SRYGEKVVLRILDNSATQLGLNKLITDPETLQIVQDMVARPFGLILVTGPTGSGKTTTLYSALAERNDPGVNISTAEDPI
EYTLPGLTQVQVIREKGMDFASILRAFLRQDPDVILVGETRDKETAKTAIEAALTGHLVLTTLHTNDAASAIARLDEMGI
ETFMVSSSLLGVLAQRLVRRVCPDCRIPFTPTSDELARFGLSSSKEIDVVFYKANSLSPDEVAQAGGNVCPTCKGVGYKG
RCGVYEVMRITERLQTLITEGAPTERIKEVAVEEGMKTLLAYSLNLVRQGSTTLEEVERVTLTDSGVESELKAKRKTSLT
CRCCGAGLKQEWLDCPYCTTPRFQD

Nucleotide


Download         Length: 1998 bp        

>NTDB_id=763116 OXH18_RS21285 WP_268609473.1 4944108..4946105(+) (pilB/pilB1) [Thermocoleostomius sinensis A174]
ATGGCTAACTCCTCGTCGCCACGGCGTGCCCTGGTCGTTCAACAAAATTTTTCCCCTTTCAGCAATAAGCTCGTTAAATC
CGGTTTTGTTAGCGGTGAACAGATGCAGCAAGCGCTGATTGAAAGCCGCAAAACAGGACGCCCGCTGACTGATATTCTGG
CAATGATGACTGGGCAGCAGCTTCCGCTAGATCTATTGCGCCAGTACAAGAAGCAGCAACTGTTTGAGCTAAAGATTCTC
TACGGCGTCGAATCGCTCGATCCCGAAATTAGTCAAGTTAATTCAGCACAGATGCATTTGCTGATTGAAACGCTGTTTCC
GATCGACCTGTGCCGTCGCTATCGTCTCATTCCCCTGTCTCGCAGCGAAACTGAACCGCCATTTGTGTTAGTGGCAATGG
TTGATCCAGATGATCTATCCGCCCAGGACGATCTCAACCGCATTCTCCGACCGCAGGGGTTAGGGCTGCAACGACTGGTG
ATTACGGCTGAGGACTATCAGCGCATTATTTCTCAGTACCTTGACGAACAGGTTGAAAAACAGAAGCAGCTTGAGTTCCA
AGCCAAAGTTGATGTTAAAAGCGATATTGAAAACCTAGACTATCTCGATCTAGAAGATACAGTCGATGACGCCGATGCTA
ATCTGGATGCCGCGCTCCAAGATCCGGAAGCCGCTCCGATCGTCGCCCTAGCCAACAAAATTCTGGCCAAAGCCCTTCAA
GAAGGGATTTCAGATATTCACATTGAGCCGCAAGAAGAACATTTACGCATTCGCTTTCGTAAAGACGGCGTGCTGCGGTT
GGGGTTTGAACTGCTGCCTAAAAAAATCATTCCAGCTTTAACTGCACGCTTCAAAATCATGGCAAATCTGGACATTGCCG
AGCGACGGATGCCCCAAGATGGGCGAATTCGGCGGCTGTTTGATAATCGTAAGGTAGACTTTCGGGTGAACACTTTACCC
AGCCGTTATGGTGAGAAGGTGGTTCTACGCATTCTAGACAACTCTGCGACGCAACTGGGACTCAACAAGCTAATCACCGA
TCCTGAAACCTTACAGATTGTGCAAGACATGGTGGCACGTCCCTTTGGACTGATCCTAGTAACAGGGCCCACTGGGTCAG
GGAAAACCACCACGCTGTATTCCGCCCTAGCCGAGCGTAACGACCCTGGCGTCAATATCAGTACCGCCGAAGATCCGATC
GAATACACGCTCCCCGGCTTAACGCAGGTGCAGGTTATTCGGGAAAAAGGCATGGACTTTGCTTCAATTCTGCGAGCTTT
CTTACGGCAAGACCCGGATGTGATTCTGGTGGGTGAAACACGAGACAAAGAAACCGCCAAAACGGCGATCGAAGCGGCTT
TGACGGGACACTTAGTTTTAACCACACTGCACACCAATGATGCGGCTAGTGCGATCGCCCGACTCGATGAAATGGGCATT
GAAACCTTCATGGTCTCCAGTTCACTGTTGGGAGTTCTGGCTCAGCGACTCGTGCGACGGGTCTGCCCCGATTGCCGCAT
TCCGTTTACTCCCACCTCGGATGAACTGGCCCGCTTTGGTTTGTCAAGTTCTAAAGAAATTGATGTCGTGTTTTACAAAG
CCAATAGCCTCAGCCCCGATGAAGTGGCTCAGGCAGGAGGAAATGTCTGTCCAACCTGCAAAGGCGTTGGATACAAAGGA
CGTTGCGGTGTATACGAAGTTATGCGCATCACTGAACGGCTGCAAACCCTGATTACTGAAGGTGCACCCACTGAACGCAT
TAAAGAAGTCGCTGTGGAAGAAGGCATGAAAACCTTGCTGGCCTATAGCCTCAATTTAGTTCGGCAAGGTTCCACCACCC
TAGAAGAAGTTGAGCGCGTCACTCTGACCGATTCCGGTGTCGAGTCCGAGCTAAAGGCCAAGCGTAAAACCTCACTGACC
TGCCGCTGCTGTGGAGCCGGACTCAAACAAGAGTGGCTAGATTGTCCTTACTGCACCACACCTCGATTTCAGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(233-500)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(54-178)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

66.216

100

0.663
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.032

93.233

0.364