Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   OSS47_RS13460 Genome accession   NZ_CP113096
Coordinates   2936800..2937219 (+) Length   139 a.a.
NCBI ID   WP_043318077.1    Uniprot ID   -
Organism   Pseudomonas citronellolis strain G5Li.T10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2931800..2942219
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OSS47_RS13435 (OSS47_13435) yacG 2931885..2932094 (-) 210 WP_043272268.1 DNA gyrase inhibitor YacG -
  OSS47_RS13440 (OSS47_13440) coaE 2932091..2932699 (-) 609 WP_043318067.1 dephospho-CoA kinase -
  OSS47_RS13445 (OSS47_13445) pilD 2932773..2933645 (-) 873 WP_043318069.1 prepilin peptidase Machinery gene
  OSS47_RS13450 (OSS47_13450) pilC 2933646..2934863 (-) 1218 WP_043318073.1 type II secretion system F family protein Machinery gene
  OSS47_RS13455 (OSS47_13455) pilB 2934867..2936570 (-) 1704 WP_043318075.1 type IV-A pilus assembly ATPase PilB Machinery gene
  OSS47_RS13460 (OSS47_13460) pilA/pilAI 2936800..2937219 (+) 420 WP_043318077.1 pilin Machinery gene
  OSS47_RS13465 (OSS47_13465) - 2937280..2938713 (+) 1434 WP_082033897.1 O-antigen ligase family protein -
  OSS47_RS13475 (OSS47_13475) - 2938999..2940072 (+) 1074 WP_043318080.1 diguanylate cyclase -
  OSS47_RS13480 (OSS47_13480) - 2940319..2940570 (+) 252 WP_043318085.1 type II toxin-antitoxin system HicA family toxin -
  OSS47_RS13485 (OSS47_13485) - 2940567..2940908 (+) 342 WP_043318088.1 type II toxin-antitoxin system HicB family antitoxin -
  OSS47_RS13490 (OSS47_13490) - 2940974..2941201 (+) 228 WP_009617646.1 DUF433 domain-containing protein -

Sequence

Protein


Download         Length: 139 a.a.        Molecular weight: 14204.11 Da        Isoelectric Point: 8.0995

>NTDB_id=760155 OSS47_RS13460 WP_043318077.1 2936800..2937219(+) (pilA/pilAI) [Pseudomonas citronellolis strain G5Li.T10]
MKAQKGFTLIELMIVVAIIGILAAIALPAYQDYTVRSQAAAALSEITPGKTAFEVAVNEGKTPSTTASDPGYIGVPGTTT
YCTVTATATTIACATKGGNETNFNNKNITWTRTAADGSWACTTTLAAKYRPGSCGAGGS

Nucleotide


Download         Length: 420 bp        

>NTDB_id=760155 OSS47_RS13460 WP_043318077.1 2936800..2937219(+) (pilA/pilAI) [Pseudomonas citronellolis strain G5Li.T10]
ATGAAGGCTCAAAAAGGCTTCACCCTTATCGAGTTGATGATCGTGGTCGCGATCATCGGTATTCTGGCTGCAATCGCCCT
GCCGGCCTATCAGGATTACACCGTACGTTCCCAGGCTGCTGCGGCTCTTTCCGAAATTACCCCCGGGAAAACCGCTTTTG
AGGTCGCAGTGAACGAAGGTAAGACTCCTTCGACTACTGCTAGTGATCCTGGTTATATTGGTGTTCCTGGAACTACTACC
TACTGCACGGTAACAGCTACTGCAACTACTATTGCTTGCGCCACTAAAGGCGGCAATGAGACTAACTTCAATAATAAAAA
TATTACTTGGACTCGTACTGCTGCTGATGGTTCTTGGGCCTGTACTACTACGCTTGCTGCTAAATATCGTCCAGGCAGCT
GTGGTGCAGGTGGTAGCTGA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-134)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

68.345

100

0.683
  pilA/pilAII Pseudomonas stutzeri DSM 10701

61.654

95.683

0.59
  pilA Acinetobacter baumannii strain A118

46.207

100

0.482
  comP Acinetobacter baylyi ADP1

45.578

100

0.482
  pilA Pseudomonas aeruginosa PAK

43.624

100

0.468
  pilA Ralstonia pseudosolanacearum GMI1000

38.889

100

0.453
  pilA2 Legionella pneumophila str. Paris

44.118

97.842

0.432
  pilA2 Legionella pneumophila strain ERS1305867

44.118

97.842

0.432
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

39.189

100

0.417
  pilA Vibrio cholerae strain A1552

39.189

100

0.417
  pilA Vibrio cholerae C6706

39.189

100

0.417
  pilE Neisseria gonorrhoeae strain FA1090

41.406

92.086

0.381