Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   OSS47_RS13455 Genome accession   NZ_CP113096
Coordinates   2934867..2936570 (-) Length   567 a.a.
NCBI ID   WP_043318075.1    Uniprot ID   -
Organism   Pseudomonas citronellolis strain G5Li.T10     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2929867..2941570
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OSS47_RS13420 (OSS47_13420) - 2930196..2930411 (+) 216 WP_043318062.1 hypothetical protein -
  OSS47_RS30755 - 2930792..2930902 (-) 111 Protein_2647 DNA gyrase inhibitor YacG -
  OSS47_RS13425 (OSS47_13425) - 2930927..2931336 (-) 410 Protein_2648 putative toxin-antitoxin system toxin component, PIN family -
  OSS47_RS13430 (OSS47_13430) - 2931336..2931575 (-) 240 WP_043318065.1 ribbon-helix-helix domain-containing protein -
  OSS47_RS13435 (OSS47_13435) yacG 2931885..2932094 (-) 210 WP_043272268.1 DNA gyrase inhibitor YacG -
  OSS47_RS13440 (OSS47_13440) coaE 2932091..2932699 (-) 609 WP_043318067.1 dephospho-CoA kinase -
  OSS47_RS13445 (OSS47_13445) pilD 2932773..2933645 (-) 873 WP_043318069.1 prepilin peptidase Machinery gene
  OSS47_RS13450 (OSS47_13450) pilC 2933646..2934863 (-) 1218 WP_043318073.1 type II secretion system F family protein Machinery gene
  OSS47_RS13455 (OSS47_13455) pilB 2934867..2936570 (-) 1704 WP_043318075.1 type IV-A pilus assembly ATPase PilB Machinery gene
  OSS47_RS13460 (OSS47_13460) pilA/pilAI 2936800..2937219 (+) 420 WP_043318077.1 pilin Machinery gene
  OSS47_RS13465 (OSS47_13465) - 2937280..2938713 (+) 1434 WP_082033897.1 O-antigen ligase family protein -
  OSS47_RS13475 (OSS47_13475) - 2938999..2940072 (+) 1074 WP_043318080.1 diguanylate cyclase -
  OSS47_RS13480 (OSS47_13480) - 2940319..2940570 (+) 252 WP_043318085.1 type II toxin-antitoxin system HicA family toxin -
  OSS47_RS13485 (OSS47_13485) - 2940567..2940908 (+) 342 WP_043318088.1 type II toxin-antitoxin system HicB family antitoxin -
  OSS47_RS13490 (OSS47_13490) - 2940974..2941201 (+) 228 WP_009617646.1 DUF433 domain-containing protein -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62656.60 Da        Isoelectric Point: 5.2414

>NTDB_id=760154 OSS47_RS13455 WP_043318075.1 2934867..2936570(-) (pilB) [Pseudomonas citronellolis strain G5Li.T10]
MNDQVVLSGLARQLVIAELLDERSAQQAQQQAQRNKLSLVTYLVQNKLVKSRPLTELAADQFGVAFCDLTAIDKDGIPKE
LISEKLVRQHRVIPLWRRGNKLFVGISDPTNHQAITDVQFSTGLTTEAILVEDDKLGDTIEKLFESATGGLDDLADVDLD
GLDIESGDADRKEESIGGDADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKIYRVRFRTDGMLHEAAKPPIQLASRISAR
LKVMAGLDISERRKPQDGRIKMRVSKNKSIDFRVNTLPTLWGEKIVMRILDSASAQMGIDSLGYDEDQKELYLEALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTPDINISTAEDPVEINLEGINQVNVNPRQGMDFAQALRAFLRQDPDVIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVASFNLATSTNLIIAQRLARKLCPHCKKEHDIPNETLLHEGFP
EDKIGSFKLYSPVGCENCKNGYKGRVGIYEVVKITPALQRIIMEEGNSIQIAEQARKEGFNDLRTSGLLKAMQGITSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=760154 OSS47_RS13455 WP_043318075.1 2934867..2936570(-) (pilB) [Pseudomonas citronellolis strain G5Li.T10]
ATGAACGATCAAGTCGTGCTATCCGGCCTCGCCCGTCAGTTGGTCATCGCCGAGCTACTGGACGAAAGAAGCGCTCAGCA
GGCCCAGCAGCAGGCTCAACGCAACAAACTTTCGCTGGTGACTTACCTGGTGCAGAACAAGCTGGTGAAGAGCCGGCCTC
TGACCGAGCTAGCCGCCGATCAGTTCGGTGTCGCCTTCTGCGATCTGACGGCCATCGACAAGGACGGCATTCCAAAGGAA
TTGATCAGCGAGAAGCTAGTACGTCAGCATCGCGTCATTCCGCTCTGGCGGCGCGGCAACAAGCTATTCGTAGGTATATC
TGATCCCACGAATCATCAAGCCATCACCGATGTCCAGTTCAGCACCGGCCTGACCACCGAGGCGATTCTGGTCGAGGACG
ACAAGCTCGGCGACACCATCGAAAAACTCTTCGAAAGTGCCACCGGCGGCCTGGACGATCTGGCCGATGTCGATCTGGAT
GGGCTGGATATTGAAAGTGGTGACGCAGACCGCAAGGAAGAAAGCATTGGAGGCGATGCTGACGACGCTCCGGTAGTCCG
CTTCGTCAACAAGATGCTACTGGACGCCATCAAGGGAGGTTCATCGGACCTGCACTTCGAGCCCTACGAGAAGATCTACC
GCGTACGCTTCCGTACCGACGGCATGCTGCATGAGGCGGCCAAGCCTCCTATCCAACTGGCTAGCCGTATCTCGGCGCGC
CTAAAGGTGATGGCTGGTCTGGATATTTCCGAAAGGCGCAAGCCTCAGGACGGCCGCATCAAGATGCGGGTGTCCAAGAA
CAAATCCATCGATTTCCGCGTCAACACCCTACCTACTCTGTGGGGGGAGAAGATCGTGATGCGGATCCTCGACTCGGCCA
GCGCGCAAATGGGGATCGACTCGCTCGGCTACGACGAGGATCAGAAAGAGCTCTACCTGGAGGCACTCAAGCAACCTCAG
GGCATGATTCTGGTGACAGGTCCGACCGGCTCCGGCAAGACCGTCTCCCTGTATACCGGCCTGAATATCCTCAATACTCC
GGACATCAATATCTCCACCGCCGAAGACCCGGTGGAGATCAACCTTGAGGGCATCAACCAGGTCAACGTCAACCCCCGCC
AAGGCATGGACTTCGCCCAGGCGCTGCGTGCCTTCCTGCGCCAGGACCCCGATGTGATCATGGTCGGCGAGATTCGCGAC
CTGGAAACCGCCGAGATTGCCATCAAGGCGGCGCAGACCGGCCACATGGTGATGTCCACCCTGCACACCAACAGTGCCGC
GGAAACCCTGACACGCCTGCTGAACATGGGCGTGGCCTCCTTCAACCTGGCCACCTCCACCAACCTGATCATCGCCCAGC
GCCTGGCGCGCAAGCTCTGCCCGCACTGCAAGAAGGAACACGACATCCCCAACGAAACCCTCCTCCACGAAGGCTTCCCG
GAGGACAAGATCGGCAGCTTCAAGCTGTATTCCCCCGTGGGGTGTGAAAATTGCAAGAACGGCTACAAGGGCCGCGTGGG
TATTTATGAAGTGGTTAAAATCACGCCGGCGCTGCAGCGGATTATCATGGAGGAAGGCAACTCCATTCAGATCGCGGAGC
AAGCCCGCAAAGAAGGCTTCAATGACCTGCGCACCTCGGGCCTGCTCAAGGCCATGCAAGGCATTACCAGCCTCGAGGAA
GTCAACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.876

99.647

0.577
  pilB Acinetobacter baylyi ADP1

57.371

99.295

0.57
  pilB Legionella pneumophila strain ERS1305867

54.69

99.647

0.545
  pilB Vibrio cholerae strain A1552

50.267

98.942

0.497
  pilF Neisseria gonorrhoeae MS11

49.556

99.295

0.492
  pilB Vibrio parahaemolyticus RIMD 2210633

51.381

95.767

0.492
  pilB Vibrio campbellii strain DS40M4

49.291

99.471

0.49
  pilF Thermus thermophilus HB27

39.123

100

0.393
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.698

93.298

0.37