Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OQ483_RS19455 Genome accession   NZ_CP110985
Coordinates   4074702..4075682 (-) Length   326 a.a.
NCBI ID   WP_047364541.1    Uniprot ID   -
Organism   Enterobacter bugandensis strain E105227     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4069702..4080682
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OQ483_RS19425 (OQ483_19415) - 4070584..4071081 (+) 498 WP_028014364.1 SprT family zinc-dependent metalloprotease -
  OQ483_RS19430 (OQ483_19420) endA 4071176..4071883 (+) 708 WP_309257511.1 deoxyribonuclease I -
  OQ483_RS19435 (OQ483_19425) rsmE 4071935..4072666 (+) 732 WP_041908327.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  OQ483_RS19440 (OQ483_19430) gshB 4072686..4073633 (+) 948 WP_033146605.1 glutathione synthase -
  OQ483_RS19445 (OQ483_19435) - 4073716..4074276 (+) 561 WP_028014368.1 YqgE/AlgH family protein -
  OQ483_RS19450 (OQ483_19440) ruvX 4074276..4074692 (+) 417 WP_021242076.1 Holliday junction resolvase RuvX -
  OQ483_RS19455 (OQ483_19445) pilT 4074702..4075682 (-) 981 WP_047364541.1 type IV pilus twitching motility protein PilT Machinery gene
  OQ483_RS19460 (OQ483_19450) - 4075700..4076401 (+) 702 WP_309257512.1 YggS family pyridoxal phosphate-dependent enzyme -
  OQ483_RS19465 (OQ483_19455) - 4076423..4076989 (+) 567 WP_045260768.1 YggT family protein -
  OQ483_RS19470 (OQ483_19460) yggU 4076986..4077282 (+) 297 WP_014885193.1 DUF167 family protein YggU -
  OQ483_RS19475 (OQ483_19465) - 4077286..4077879 (+) 594 WP_010435473.1 XTP/dITP diphosphatase -
  OQ483_RS19480 (OQ483_19470) hemW 4077872..4079014 (+) 1143 WP_192971636.1 radical SAM family heme chaperone HemW -
  OQ483_RS19485 (OQ483_19475) - 4079078..4079416 (+) 339 WP_309257513.1 endonuclease domain-containing protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35611.67 Da        Isoelectric Point: 6.3904

>NTDB_id=757301 OQ483_RS19455 WP_047364541.1 4074702..4075682(-) (pilT) [Enterobacter bugandensis strain E105227]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRLGRLEPAPFPPPDVGALLKAWLNDEQQGAWWANGQVDFAVTVAGDQRLRG
SAFKQMKGISVTLRLLPRSCPQLPSLGVPRAIPELLSSDNGLILVTGATGSGKSTTLAAMVDFLNRHTDGHILTLEDPVE
FMYQSERCLIQQREIGQHSPSFAEALRSALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLLPDLQGGRVALYELLVNTAAAANLIREGRTWQLPGIIQTGQQAGMQNFDQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=757301 OQ483_RS19455 WP_047364541.1 4074702..4075682(-) (pilT) [Enterobacter bugandensis strain E105227]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCTGATCTACACCTGTGCAGTGATTCACCACCGCG
CTGGCGCAGATTGGGCCGCCTTGAGCCGGCCCCGTTTCCGCCGCCGGACGTCGGGGCGTTGCTAAAGGCGTGGCTCAACG
ACGAACAGCAGGGCGCATGGTGGGCAAACGGGCAGGTCGATTTTGCCGTGACGGTCGCAGGAGACCAGCGTCTGCGCGGC
AGTGCGTTTAAGCAGATGAAGGGCATCTCCGTTACCCTGCGGCTGTTGCCGCGTTCCTGTCCGCAGCTCCCTTCGCTTGG
GGTGCCGCGCGCTATTCCCGAACTGTTGTCCAGCGACAACGGGTTGATTCTGGTAACGGGAGCGACCGGCAGCGGTAAAT
CCACCACGCTGGCCGCAATGGTCGACTTCCTCAACCGCCATACTGACGGCCATATCCTCACGCTTGAAGATCCGGTGGAG
TTTATGTACCAGAGTGAACGTTGCCTGATTCAGCAGCGGGAGATAGGCCAGCACAGCCCGTCATTTGCAGAGGCGCTGCG
CAGTGCGTTACGCGAGGACCCGGACGTTATTTTGCTGGGGGAGCTGCGCGACAGCGAAACGATCCGCCTGGCGCTGACGG
CGGCGGAGACCGGGCATCTGGTGCTGGCGACGCTGCATACGCGCGGGGCAGCACAGGCGATTGAACGGCTGGTCGATACG
TTCCCGGCGCAGGAGAAAGATCCGGTGCGTAACCAGCTGGCTGGCAGCCTGCGGGCAGTGCTGGCGCAAAAGCTGCTTCC
CGATCTTCAGGGCGGGCGCGTCGCGCTGTACGAGCTGCTGGTAAACACTGCGGCGGCGGCGAATCTGATTCGTGAGGGGA
GGACGTGGCAGCTGCCCGGCATCATTCAAACCGGCCAGCAGGCGGGAATGCAGAACTTTGACCAGAGCCTGGCGGAGAGA
CGGGCACAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(90-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.153

100

0.503
  pilT Vibrio cholerae strain A1552

50.153

100

0.503
  pilT Pseudomonas stutzeri DSM 10701

48.624

100

0.488
  pilT Legionella pneumophila strain Lp02

49.379

98.773

0.488
  pilT Legionella pneumophila strain ERS1305867

49.379

98.773

0.488
  pilT Neisseria meningitidis 8013

47.866

100

0.482
  pilT Acinetobacter baumannii strain A118

48.012

100

0.482
  pilT Acinetobacter baylyi ADP1

48.012

100

0.482
  pilT Acinetobacter baumannii D1279779

48.012

100

0.482
  pilT Neisseria gonorrhoeae MS11

47.561

100

0.479
  pilT Pseudomonas aeruginosa PAK

47.706

100

0.479
  pilT Acinetobacter nosocomialis M2

48.438

98.16

0.475
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.547

98.773

0.42
  pilU Pseudomonas stutzeri DSM 10701

39.091

100

0.396
  pilU Vibrio cholerae strain A1552

39.441

98.773

0.39