Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OOK60_RS07085 Genome accession   NZ_CP110848
Coordinates   1666222..1667358 (-) Length   378 a.a.
NCBI ID   WP_265903650.1    Uniprot ID   -
Organism   Trichothermofontia sichuanensis B231     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1661222..1672358
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OOK60_RS07060 (OOK60_07060) - 1662549..1663124 (-) 576 WP_265903645.1 pentapeptide repeat-containing protein -
  OOK60_RS07065 (OOK60_07065) - 1663201..1663359 (-) 159 WP_265903646.1 hypothetical protein -
  OOK60_RS07070 (OOK60_07070) - 1663472..1664080 (+) 609 WP_265903647.1 hypothetical protein -
  OOK60_RS07075 (OOK60_07075) - 1664189..1664788 (-) 600 WP_265903648.1 hypothetical protein -
  OOK60_RS07080 (OOK60_07080) - 1664825..1666039 (-) 1215 WP_265903649.1 type II secretion system F family protein -
  OOK60_RS07085 (OOK60_07085) pilT 1666222..1667358 (-) 1137 WP_265903650.1 type IV pilus twitching motility protein PilT Machinery gene
  OOK60_RS07090 (OOK60_07090) pilB/pilB1 1667564..1669579 (-) 2016 WP_265903651.1 GspE/PulE family protein Machinery gene
  OOK60_RS07095 (OOK60_07095) grpE 1670291..1671106 (+) 816 WP_265903652.1 nucleotide exchange factor GrpE -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41404.60 Da        Isoelectric Point: 6.7592

>NTDB_id=756588 OOK60_RS07085 WP_265903650.1 1666222..1667358(-) (pilT) [Trichothermofontia sichuanensis B231]
MELMIEDLMEQLVEMGGSDLHLSAGLPPYFRLSGKLTPIGNEPLSAEECQRLIFSMLNNTQRKNLEQNWELDCSYGVKGL
ARFRVNVYKDRGTYAACLRALSSKIPSFEKLGLPEVVREMSEKPRGLILVTGPTGSGKSTTLASVIDYINRTRAEHILTV
EDPIEFVYEPIKSLIHQRQLHEDTKSFANALKAALREDPDIILVGEMRDLETISLAITAAETGHLVFGTLHTSSAAQTVD
RIIDVFPADRQTQVRVQLSNSLVAVFSQTLVPRKNPKPGEFGRVMAQEILVVTPAISNLIREGKTAQIYSAIQTGAKLGM
QTLERVLADLYLNGTISFDAAMSKTSKPEELQRLIGGATPKGPSTPSPSASKVVQVSG

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=756588 OOK60_RS07085 WP_265903650.1 1666222..1667358(-) (pilT) [Trichothermofontia sichuanensis B231]
ATGGAGTTAATGATTGAAGATTTGATGGAGCAACTGGTGGAGATGGGAGGCTCGGACCTGCATCTCTCAGCCGGCTTACC
TCCCTATTTCCGGTTGAGCGGGAAATTAACCCCGATCGGGAATGAACCTCTCAGTGCAGAGGAATGCCAGCGGCTGATTT
TCAGTATGCTCAACAACACCCAGCGGAAGAATTTGGAACAGAACTGGGAATTGGACTGCTCCTACGGGGTCAAGGGACTC
GCCCGGTTCCGGGTGAACGTCTACAAAGATCGGGGGACCTACGCTGCTTGTTTGCGTGCTCTGAGTTCCAAGATCCCTAG
CTTTGAAAAGTTAGGCCTTCCGGAAGTAGTCCGGGAAATGTCGGAAAAGCCGCGTGGGCTGATTCTGGTGACAGGACCCA
CTGGATCGGGCAAAAGTACGACCCTGGCTTCAGTGATTGACTATATTAACCGCACCCGTGCTGAACATATTTTAACGGTT
GAGGACCCAATCGAATTTGTCTACGAGCCGATCAAGAGTTTGATCCACCAGCGGCAACTTCACGAAGACACCAAGAGCTT
TGCCAACGCCCTCAAGGCTGCCCTGCGGGAAGACCCCGACATCATCCTAGTGGGGGAAATGCGAGACCTGGAAACCATTT
CGCTGGCCATCACCGCGGCTGAAACTGGGCACTTGGTCTTTGGTACCCTCCACACCAGTTCCGCAGCCCAAACCGTAGAC
CGGATTATTGACGTATTCCCTGCCGATCGCCAAACTCAGGTGCGGGTACAGTTATCCAACTCCCTAGTGGCGGTATTCAG
CCAGACGTTGGTTCCCCGCAAGAACCCGAAGCCGGGTGAATTTGGGCGGGTAATGGCTCAGGAAATTCTGGTGGTTACGC
CGGCTATTTCTAACCTTATTCGGGAAGGGAAAACTGCCCAGATCTACTCAGCGATTCAAACCGGGGCCAAACTGGGGATG
CAAACGCTAGAGCGGGTATTGGCCGACTTATATCTTAACGGCACCATTAGTTTTGATGCGGCTATGTCCAAGACCTCCAA
ACCGGAAGAACTCCAGCGTTTGATCGGCGGTGCAACCCCGAAAGGCCCCAGTACACCGTCACCCAGTGCGAGCAAGGTTG
TTCAGGTTTCTGGGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(107-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

55.043

91.799

0.505
  pilT Acinetobacter baylyi ADP1

54.755

91.799

0.503
  pilT Legionella pneumophila strain Lp02

54.783

91.27

0.5
  pilT Legionella pneumophila strain ERS1305867

54.783

91.27

0.5
  pilT Pseudomonas stutzeri DSM 10701

54.467

91.799

0.5
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.269

98.413

0.495
  pilT Acinetobacter nosocomialis M2

53.602

91.799

0.492
  pilT Acinetobacter baumannii strain A118

53.602

91.799

0.492
  pilT Acinetobacter baumannii D1279779

53.602

91.799

0.492
  pilT Neisseria gonorrhoeae MS11

51.862

92.328

0.479
  pilT Neisseria meningitidis 8013

51.576

92.328

0.476
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.765

89.947

0.466
  pilT Vibrio cholerae strain A1552

51.765

89.947

0.466
  pilU Pseudomonas stutzeri DSM 10701

38.503

98.942

0.381