Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   OOK60_RS07090 Genome accession   NZ_CP110848
Coordinates   1667564..1669579 (-) Length   671 a.a.
NCBI ID   WP_265903651.1    Uniprot ID   -
Organism   Trichothermofontia sichuanensis B231     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1662564..1674579
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OOK60_RS07065 (OOK60_07065) - 1663201..1663359 (-) 159 WP_265903646.1 hypothetical protein -
  OOK60_RS07070 (OOK60_07070) - 1663472..1664080 (+) 609 WP_265903647.1 hypothetical protein -
  OOK60_RS07075 (OOK60_07075) - 1664189..1664788 (-) 600 WP_265903648.1 hypothetical protein -
  OOK60_RS07080 (OOK60_07080) - 1664825..1666039 (-) 1215 WP_265903649.1 type II secretion system F family protein -
  OOK60_RS07085 (OOK60_07085) pilT 1666222..1667358 (-) 1137 WP_265903650.1 type IV pilus twitching motility protein PilT Machinery gene
  OOK60_RS07090 (OOK60_07090) pilB/pilB1 1667564..1669579 (-) 2016 WP_265903651.1 GspE/PulE family protein Machinery gene
  OOK60_RS07095 (OOK60_07095) grpE 1670291..1671106 (+) 816 WP_265903652.1 nucleotide exchange factor GrpE -
  OOK60_RS07100 (OOK60_07100) dnaK 1671289..1673223 (+) 1935 WP_265903653.1 molecular chaperone DnaK -
  OOK60_RS07105 (OOK60_07105) dnaJ 1673362..1674495 (+) 1134 WP_265903654.1 molecular chaperone DnaJ -

Sequence

Protein


Download         Length: 671 a.a.        Molecular weight: 74455.49 Da        Isoelectric Point: 4.9717

>NTDB_id=756589 OOK60_RS07090 WP_265903651.1 1667564..1669579(-) (pilB/pilB1) [Trichothermofontia sichuanensis B231]
MTNSSSARRALIAHNTLSPFGNQLVQAGFADANQMSQALVESRKSGRPLPEILSELTGKVLPPDLLRQYKKQQLFELKIL
YGIESLDPEISETSPEEISHLIDTLISVDVCRRYRLIPLTKDKNQDPPLITIAMVEPDNLLALDELKKILKGFQIRKVVI
TPEDYEQLLGRYMDEVVKKAGTAAAQIREIRTEDLEIKESDLANYDLEDADEEDADVDLNAALGEAESAPVVRMVNQILA
RALSEGVSDIHIEPQEEYLRVRFRKDGVLQEPLPKLPKKIIPAIVSRFKIIADLDIAERRLPQDGRIRRKFQGRNVDFRV
NTLPSRYGEKVVLRILDNSSTQLGLDKLITDPETLALVREMASRPFGLILVTGPTGSGKSTTLYSILAERNDPGVNISTA
EDPIEYALPGITQVQVIREKGMDFSSILRAFLRQDPDIILVGETRDKETAKTAIEAALTGHLVLTTLHTNDAAGAIARLD
EMGVEPFMVSGALLGVVAQRLMRRVCDKCCIAYTPTPEELARYGLTASKEVGITFYKANTLSNDQIQAAKDAGTLCPKCN
GVGYKGRCGVYEVLRTTERLQVAINEGAPTERIKEIAVEEGMKTLLAYSLDLVRQGLTTFEEVERVTFTDTGLEAELKAK
RKTSLTCRTCGAGLQPEWMDCPYCLTPRFTD

Nucleotide


Download         Length: 2016 bp        

>NTDB_id=756589 OOK60_RS07090 WP_265903651.1 1667564..1669579(-) (pilB/pilB1) [Trichothermofontia sichuanensis B231]
ATGACCAACTCCTCCTCTGCACGGCGGGCCTTAATTGCCCATAATACCCTTTCCCCCTTTGGCAATCAGCTCGTTCAAGC
AGGGTTTGCCGATGCGAATCAAATGAGTCAGGCCCTGGTTGAGAGTCGCAAATCGGGCAGGCCCCTGCCTGAGATTCTGA
GTGAGTTAACCGGCAAGGTTTTACCGCCGGACTTGCTGCGGCAATACAAGAAGCAGCAATTGTTTGAACTTAAAATTCTC
TACGGTATTGAGTCCCTCGATCCGGAAATTAGTGAAACCTCGCCAGAGGAGATCAGCCACCTGATCGACACGCTCATCAG
CGTTGATGTGTGTCGTCGCTACCGCCTAATTCCCTTGACCAAGGATAAGAATCAAGATCCCCCCCTCATTACGATTGCAA
TGGTGGAGCCGGATAATCTGTTGGCCTTGGACGAGTTAAAGAAGATTCTGAAGGGCTTCCAGATTCGCAAGGTGGTGATT
ACCCCAGAGGATTACGAACAACTTCTGGGACGGTACATGGACGAGGTCGTCAAAAAAGCCGGGACCGCTGCTGCCCAAAT
CCGCGAGATTCGGACGGAAGACTTGGAGATTAAGGAGAGTGACCTAGCGAATTACGATCTCGAAGATGCGGATGAAGAGG
ATGCCGATGTTGACCTGAATGCTGCCCTGGGTGAGGCCGAAAGTGCCCCTGTGGTGCGCATGGTGAATCAAATTCTGGCG
CGGGCCTTGAGCGAAGGGGTATCGGATATTCACATTGAACCCCAGGAAGAATACCTGCGGGTGCGCTTCCGTAAGGACGG
GGTGCTGCAAGAGCCGCTGCCAAAGCTACCCAAGAAAATTATTCCGGCAATTGTCTCGCGCTTTAAGATTATTGCCGATC
TCGATATTGCCGAGCGGCGGTTGCCCCAGGATGGTCGGATTCGCCGCAAGTTCCAGGGACGCAACGTGGACTTTCGGGTG
AATACCCTACCGAGTCGGTATGGCGAGAAGGTGGTGCTCCGGATTCTGGACAACTCCTCGACCCAGCTCGGCCTCGACAA
ACTTATTACCGATCCCGAAACCCTGGCCCTGGTCCGGGAAATGGCCAGTCGTCCCTTTGGCTTGATTCTGGTGACGGGGC
CAACGGGTTCCGGCAAATCGACCACCCTGTATTCCATCCTGGCGGAGCGCAATGATCCAGGGGTCAATATCAGTACGGCA
GAGGATCCGATCGAATACGCGCTGCCGGGCATTACGCAGGTACAGGTGATTCGGGAGAAGGGGATGGACTTTTCCTCGAT
CCTGCGGGCGTTTCTACGGCAAGACCCGGACATCATTCTGGTGGGGGAAACCCGGGACAAGGAAACGGCCAAAACGGCGA
TCGAAGCGGCTTTAACTGGGCACCTGGTATTAACCACTCTGCACACCAACGATGCTGCGGGTGCGATCGCCCGTTTGGAT
GAAATGGGGGTTGAACCCTTCATGGTCTCCGGTGCCTTGTTGGGAGTTGTAGCCCAGCGCTTGATGCGTCGAGTTTGCGA
CAAATGTTGTATTGCCTATACCCCTACACCGGAGGAGTTGGCGCGCTATGGTCTGACGGCTTCCAAGGAAGTGGGCATTA
CCTTTTACAAAGCCAATACCCTCAGTAATGATCAAATCCAGGCGGCTAAAGATGCGGGAACGCTTTGTCCCAAGTGTAAT
GGCGTGGGTTACAAGGGACGGTGTGGGGTCTACGAGGTGTTGCGGACCACTGAACGTCTACAGGTGGCAATCAACGAAGG
GGCACCGACTGAGCGGATTAAGGAAATCGCGGTGGAGGAAGGCATGAAAACCTTGCTAGCCTATAGTCTTGACTTGGTAC
GCCAGGGACTGACCACGTTTGAGGAAGTGGAGCGGGTAACCTTTACGGATACGGGTTTGGAGGCTGAACTCAAGGCCAAA
CGCAAGACCTCCCTGACCTGTCGCACTTGCGGGGCGGGCCTGCAACCGGAGTGGATGGATTGTCCCTATTGCCTCACGCC
ACGATTTACCGATTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(24-175)

Type II/IV secretion system protein (T2SSE)

(237-504)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

65.113

99.106

0.645
  pilF Thermus thermophilus HB27

40.887

90.76

0.371