Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OKX07_RS09820 Genome accession   NZ_CP110806
Coordinates   2195980..2197095 (+) Length   371 a.a.
NCBI ID   WP_416220872.1    Uniprot ID   -
Organism   Cellulomonas sp. S1-8     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2190980..2202095
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OKX07_RS09800 (OKX07_09800) ruvX 2191054..2191638 (+) 585 WP_416220857.1 Holliday junction resolvase RuvX -
  OKX07_RS09805 (OKX07_09805) mltG 2191646..2192857 (+) 1212 WP_265631713.1 endolytic transglycosylase MltG -
  OKX07_RS09810 (OKX07_09810) - 2192854..2193699 (+) 846 WP_265631714.1 shikimate dehydrogenase -
  OKX07_RS09815 (OKX07_09815) - 2193831..2195501 (+) 1671 WP_265631715.1 GspE/PulE family protein -
  OKX07_RS09820 (OKX07_09820) pilT 2195980..2197095 (+) 1116 WP_416220872.1 type IV pilus twitching motility protein PilT Machinery gene
  OKX07_RS09825 (OKX07_09825) - 2197095..2198321 (+) 1227 WP_265631716.1 type II secretion system F family protein -
  OKX07_RS09830 (OKX07_09830) - 2198471..2198869 (+) 399 WP_265631717.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  OKX07_RS09835 (OKX07_09835) - 2199017..2200390 (+) 1374 WP_265631718.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  OKX07_RS09840 (OKX07_09840) - 2200387..2201079 (+) 693 WP_265631719.1 PulJ/GspJ family protein -

Sequence

Protein


Download         Length: 371 a.a.        Molecular weight: 40491.22 Da        Isoelectric Point: 8.9928

>NTDB_id=756236 OKX07_RS09820 WP_416220872.1 2195980..2197095(+) (pilT) [Cellulomonas sp. S1-8]
MHRLGASDVHFTTGSPPMVRISGALRPLDQFGVLTPDPLRRSLYSFLTQKQREKYEANLELDISYAVRGLARFRVNLYQQ
RESIGAAFRVIPYEIKPLEDLGVPTSVGSFAGLPRGLVLVTGPTGSGKSTTLASIIDLANRTREDHIMTVEDPIEFLHRH
KKCLVNQREVGADTHSFTNALKHVLRQDPDIILVGEMRDLETISVALTAAETGHLVFATLHTQDASQTIDRIIDVFPSGQ
QAQIRTQLASAIQGVVCQTLCKKATGVGRVVATEVMLATPAIRNLVREGKTHQIYSAMQAGADNGMHTMDQNLAELVKRG
KITYEVGLDKAHHIEDFNKLVGRTSRVRQGASSTQSFGGTTAPGISSLGGY

Nucleotide


Download         Length: 1116 bp        

>NTDB_id=756236 OKX07_RS09820 WP_416220872.1 2195980..2197095(+) (pilT) [Cellulomonas sp. S1-8]
ATGCACCGGCTCGGCGCGTCCGACGTGCACTTCACGACGGGGTCGCCGCCGATGGTCCGGATCTCGGGTGCCCTGCGCCC
GCTGGACCAGTTCGGCGTCCTGACCCCCGACCCGTTGCGGCGCAGCCTCTACTCGTTCCTCACGCAGAAGCAGCGGGAGA
AGTACGAGGCGAACCTCGAGCTCGACATCTCCTACGCCGTGCGTGGGCTGGCGCGGTTCCGCGTCAACCTGTACCAGCAG
CGGGAGTCCATCGGCGCCGCGTTCCGTGTGATCCCCTACGAGATCAAGCCGCTCGAGGACCTCGGGGTGCCCACGTCGGT
CGGGTCCTTCGCGGGCCTCCCGCGCGGGCTGGTCCTGGTCACGGGGCCCACGGGCTCCGGCAAGTCCACGACCCTCGCCT
CGATCATCGACCTCGCCAACCGCACGCGCGAGGACCACATCATGACGGTCGAGGACCCCATCGAGTTCCTCCACCGTCAC
AAGAAGTGCCTGGTCAACCAGCGCGAGGTCGGTGCGGACACGCACTCGTTCACCAACGCGCTCAAGCACGTGCTGCGCCA
GGACCCCGACATCATCCTGGTCGGCGAGATGCGTGACCTCGAGACCATCTCGGTGGCGCTCACCGCCGCCGAGACGGGTC
ACCTCGTGTTCGCGACCCTGCACACGCAGGACGCCTCCCAGACGATCGACCGGATCATCGACGTCTTCCCGTCGGGTCAG
CAGGCGCAGATCCGGACGCAGCTCGCCAGCGCCATCCAGGGCGTCGTCTGCCAGACGCTGTGCAAGAAGGCGACCGGGGT
GGGCCGCGTGGTCGCGACCGAGGTGATGCTCGCCACGCCTGCCATCCGCAACCTCGTCCGCGAGGGCAAGACGCACCAGA
TCTACTCGGCCATGCAGGCCGGCGCCGACAACGGCATGCACACGATGGACCAGAACCTGGCCGAGCTGGTCAAGCGCGGC
AAGATCACGTACGAGGTCGGCCTGGACAAGGCGCACCACATCGAGGACTTCAACAAGCTCGTGGGGCGCACGTCGCGCGT
GCGCCAGGGCGCGTCCTCGACGCAGAGCTTCGGCGGGACGACCGCCCCCGGCATCTCCAGCCTCGGAGGTTACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(109-253)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52

94.34

0.491
  pilT Acinetobacter baumannii D1279779

54.354

89.757

0.488
  pilT Acinetobacter baumannii strain A118

54.354

89.757

0.488
  pilT Acinetobacter nosocomialis M2

54.354

89.757

0.488
  pilT Acinetobacter baylyi ADP1

53.916

89.488

0.482
  pilT Legionella pneumophila strain ERS1305867

53.134

90.296

0.48
  pilT Legionella pneumophila strain Lp02

53.134

90.296

0.48
  pilT Pseudomonas aeruginosa PAK

53.453

89.757

0.48
  pilT Pseudomonas stutzeri DSM 10701

52.252

89.757

0.469
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

53.125

86.253

0.458
  pilT Vibrio cholerae strain A1552

53.125

86.253

0.458
  pilT Neisseria meningitidis 8013

48.649

89.757

0.437
  pilT Neisseria gonorrhoeae MS11

48.348

89.757

0.434
  pilU Vibrio cholerae strain A1552

41.349

91.914

0.38
  pilU Pseudomonas stutzeri DSM 10701

40.653

90.836

0.369