Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GN112_RS28425 Genome accession   NZ_AP021879
Coordinates   6492627..6493709 (+) Length   360 a.a.
NCBI ID   WP_155313254.1    Uniprot ID   A0A5K8ALP5
Organism   Desulfosarcina ovata subsp. ovata strain oXyS1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6487627..6498709
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GN112_RS28400 (DSCOOX_56990) proC 6487736..6488590 (-) 855 WP_155313249.1 pyrroline-5-carboxylate reductase -
  GN112_RS28405 (DSCOOX_57000) - 6488860..6490998 (+) 2139 WP_155313250.1 aldehyde ferredoxin oxidoreductase family protein -
  GN112_RS28410 (DSCOOX_57010) thiS 6491073..6491303 (+) 231 WP_155313251.1 sulfur carrier protein ThiS -
  GN112_RS28415 (DSCOOX_57020) - 6491576..6491875 (+) 300 WP_155313252.1 YggT family protein -
  GN112_RS28420 (DSCOOX_57030) - 6492052..6492549 (+) 498 WP_155313253.1 DivIVA domain-containing protein -
  GN112_RS28425 (DSCOOX_57040) pilT 6492627..6493709 (+) 1083 WP_155313254.1 type IV pilus twitching motility protein PilT Machinery gene
  GN112_RS28430 (DSCOOX_57050) - 6493835..6494998 (+) 1164 WP_155313255.1 type IV pilus twitching motility protein PilT -
  GN112_RS28435 (DSCOOX_57060) - 6495193..6495849 (+) 657 WP_155313256.1 zinc-ribbon domain-containing protein -
  GN112_RS28440 (DSCOOX_57080) - 6496207..6497121 (+) 915 WP_155313257.1 MBL fold metallo-hydrolase -
  GN112_RS28450 (DSCOOX_57090) folP 6497352..6498218 (-) 867 WP_155313259.1 dihydropteroate synthase -

Sequence

Protein


Download         Length: 360 a.a.        Molecular weight: 39904.99 Da        Isoelectric Point: 8.1366

>NTDB_id=75205 GN112_RS28425 WP_155313254.1 6492627..6493709(+) (pilT) [Desulfosarcina ovata subsp. ovata strain oXyS1]
MAKIDAFFKLMHEQNASDLHLLSGQPPALRIHGDIERVKYKVLDNDDLRKMLYEITPEDKIKHFEETGDVDFGYEIPGLA
RYRANYFMQKNGLGAVFREIPSAILTAEQLGLPPVMSKLASLPRGLVIVTGPTGSGKSTTLAAILDVANRTRSDHIITIE
DPIEFVHKSRGCLVNHREVGLHTKSFSAALRGALREDPDIILVGEMRDLETISLAIEASATGHLVFGTLHTTSAPKTVDR
IVEVFPAQEQAQIRSTLADGIRAIISQTLFKRIDKKGRVPALEILIATPAVRNLIREAKSHQLPSMMQTGKKYGMQLLDD
AIMDLYNKGRISADDAYTKANDKSRFRPLLKSPPTDFTEA

Nucleotide


Download         Length: 1083 bp        

>NTDB_id=75205 GN112_RS28425 WP_155313254.1 6492627..6493709(+) (pilT) [Desulfosarcina ovata subsp. ovata strain oXyS1]
ATGGCCAAAATCGATGCCTTTTTCAAACTCATGCACGAGCAGAATGCATCTGACCTCCATTTGCTATCGGGCCAGCCACC
GGCCCTGCGGATTCACGGTGACATCGAGCGGGTAAAGTACAAAGTGCTGGATAACGACGACTTGCGCAAGATGCTTTACG
AGATCACGCCTGAAGACAAGATCAAACACTTTGAAGAGACCGGCGATGTGGATTTCGGTTATGAAATTCCCGGCCTGGCC
CGTTACCGGGCCAATTATTTCATGCAAAAAAATGGCCTCGGAGCCGTTTTCCGGGAAATTCCCAGTGCGATTCTGACCGC
CGAGCAGCTGGGGTTGCCGCCGGTCATGTCCAAGCTGGCGTCGCTTCCCAGGGGGCTGGTTATTGTTACCGGGCCCACCG
GGTCCGGTAAATCCACCACCCTGGCGGCAATCCTTGATGTGGCCAACCGCACCCGCAGCGATCATATCATTACCATCGAG
GACCCCATCGAATTTGTTCACAAAAGCCGTGGCTGTCTGGTCAACCACCGCGAGGTGGGCCTACATACCAAGTCATTTAG
CGCAGCCCTGCGTGGTGCCCTGCGCGAGGACCCGGATATCATTCTGGTGGGCGAAATGCGTGATCTGGAGACCATTTCAC
TGGCCATCGAAGCCAGTGCCACCGGACACTTGGTGTTCGGTACGCTGCACACCACCAGTGCACCCAAGACCGTGGACCGT
ATCGTGGAGGTTTTTCCCGCCCAGGAGCAGGCCCAGATTCGTTCCACCCTGGCAGATGGTATCCGGGCGATTATCTCCCA
GACCCTGTTCAAGCGGATTGACAAAAAAGGACGGGTGCCGGCCCTGGAGATTCTCATTGCCACGCCAGCGGTGCGCAACC
TGATCCGCGAGGCCAAATCCCACCAGCTGCCGTCCATGATGCAGACCGGCAAGAAGTACGGCATGCAACTGCTTGACGAT
GCCATCATGGATCTCTACAACAAAGGGCGCATTTCTGCCGATGATGCCTATACCAAGGCCAATGACAAATCCCGCTTCCG
GCCGCTGCTCAAGTCTCCGCCCACCGATTTTACCGAAGCCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(116-257)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5K8ALP5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

57.784

92.778

0.536
  pilT Pseudomonas aeruginosa PAK

57.186

92.778

0.531
  pilT Legionella pneumophila strain Lp02

54.79

92.778

0.508
  pilT Legionella pneumophila strain ERS1305867

54.79

92.778

0.508
  pilT Acinetobacter nosocomialis M2

53.293

92.778

0.494
  pilT Acinetobacter baumannii D1279779

53.293

92.778

0.494
  pilT Acinetobacter baumannii strain A118

53.293

92.778

0.494
  pilT Acinetobacter baylyi ADP1

53.293

92.778

0.494
  pilT Neisseria meningitidis 8013

52.905

90.833

0.481
  pilT Neisseria gonorrhoeae MS11

52.599

90.833

0.478
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.07

90.833

0.464
  pilT Vibrio cholerae strain A1552

51.07

90.833

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.813

95.278

0.456
  pilU Pseudomonas stutzeri DSM 10701

39.255

96.944

0.381
  pilU Acinetobacter baylyi ADP1

38.439

96.111

0.369

Multiple sequence alignment