Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   FQS62_RS17635 Genome accession   NZ_CP109812
Coordinates   3810098..3810550 (-) Length   150 a.a.
NCBI ID   WP_208043037.1    Uniprot ID   -
Organism   Stenotrophomonas sp. SBJS02     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 3796924..3809992 3810098..3810550 flank 106


Gene organization within MGE regions


Location: 3796924..3810550
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FQS62_RS17575 (FQS62_017575) - 3796924..3797598 (+) 675 WP_141097651.1 hypothetical protein -
  FQS62_RS17580 (FQS62_017580) - 3797592..3798605 (+) 1014 WP_049447473.1 hypothetical protein -
  FQS62_RS17585 (FQS62_017585) - 3798599..3799615 (+) 1017 WP_208042994.1 TraB/GumN family protein -
  FQS62_RS17590 (FQS62_017590) - 3799934..3800203 (+) 270 WP_049447475.1 hypothetical protein -
  FQS62_RS17595 (FQS62_017595) - 3800255..3802012 (+) 1758 WP_208042995.1 ABC transporter transmembrane domain-containing protein -
  FQS62_RS17600 (FQS62_017600) sucD 3802130..3803005 (-) 876 WP_017356169.1 succinate--CoA ligase subunit alpha -
  FQS62_RS17605 (FQS62_017605) sucC 3803026..3804195 (-) 1170 WP_208042996.1 ADP-forming succinate--CoA ligase subunit beta -
  FQS62_RS17610 (FQS62_017610) - 3804601..3806214 (+) 1614 WP_049447477.1 ATP-binding protein -
  FQS62_RS17615 (FQS62_017615) pilR 3806275..3807648 (+) 1374 WP_049447478.1 sigma-54 dependent transcriptional regulator Regulator
  FQS62_RS17620 (FQS62_017620) pilB 3807754..3809482 (-) 1729 Protein_3445 type IV-A pilus assembly ATPase PilB -
  FQS62_RS22305 - 3809576..3809992 (-) 417 WP_208043038.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  FQS62_RS17635 (FQS62_017635) pilA2 3810098..3810550 (-) 453 WP_208043037.1 pilin Machinery gene

Sequence

Protein


Download         Length: 150 a.a.        Molecular weight: 15164.52 Da        Isoelectric Point: 7.8202

>NTDB_id=749532 FQS62_RS17635 WP_208043037.1 3810098..3810550(-) (pilA2) [Stenotrophomonas sp. SBJS02]
MKNQKGFTLIELMIVVAIIAILAAIALPAYQDYTIRAKVSEGLVQADAAKLAVAETAQSQGILATAVTDEAAAGYVSKAT
NNVASVGIANGVITITTKATGAKVQPILKLTPSQTDLDSPITWACTFTAGEAKHVPASCRTAGAGAPPAG

Nucleotide


Download         Length: 453 bp        

>NTDB_id=749532 FQS62_RS17635 WP_208043037.1 3810098..3810550(-) (pilA2) [Stenotrophomonas sp. SBJS02]
ATGAAGAACCAGAAGGGCTTCACCCTGATCGAACTGATGATCGTTGTTGCGATCATCGCCATCCTGGCCGCCATCGCGCT
GCCGGCTTACCAGGACTACACCATCCGTGCCAAGGTGTCGGAAGGCCTGGTCCAGGCCGACGCTGCCAAGCTCGCCGTTG
CTGAAACCGCTCAGTCGCAGGGCATATTGGCGACCGCAGTCACCGATGAGGCCGCTGCTGGTTACGTGAGCAAGGCCACG
AACAATGTAGCTTCGGTTGGCATCGCCAATGGCGTCATCACCATCACCACTAAGGCAACCGGTGCAAAGGTCCAGCCGAT
TCTCAAGCTGACCCCGAGCCAGACTGATTTGGATTCTCCGATCACTTGGGCTTGCACCTTCACCGCTGGTGAAGCGAAGC
ACGTGCCGGCTAGCTGCCGCACTGCTGGTGCTGGCGCTCCGCCTGCCGGCTGA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(36-140)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

60.563

94.667

0.573
  pilA2 Legionella pneumophila strain ERS1305867

59.859

94.667

0.567
  comP Acinetobacter baylyi ADP1

46.405

100

0.473
  pilA Ralstonia pseudosolanacearum GMI1000

41.718

100

0.453
  pilE Neisseria gonorrhoeae MS11

38.415

100

0.42
  pilE Neisseria gonorrhoeae strain FA1090

40.127

100

0.42
  pilA Haemophilus influenzae 86-028NP

41.497

98

0.407
  pilA Acinetobacter baumannii strain A118

36.306

100

0.38
  pilA Vibrio cholerae strain A1552

37.5

100

0.38
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

37.5

100

0.38
  pilA Vibrio cholerae C6706

37.5

100

0.38
  pilA Pseudomonas aeruginosa PAK

36.774

100

0.38
  pilA Haemophilus influenzae Rd KW20

40.876

91.333

0.373
  pilA/pilA1 Eikenella corrodens VA1

36.424

100

0.367