Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QDY29_RS04350 Genome accession   NZ_CP122816
Coordinates   880089..881069 (+) Length   326 a.a.
NCBI ID   WP_282842852.1    Uniprot ID   -
Organism   Escherichia coli strain ETEC1731     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 875089..886069
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QDY29_RS04320 (QDY29_04320) yggM 875604..876611 (+) 1008 WP_000745217.1 DUF1202 family protein -
  QDY29_RS04325 (QDY29_04325) hemW 876766..877902 (-) 1137 WP_000239928.1 radical SAM family heme chaperone HemW -
  QDY29_RS04330 (QDY29_04330) rdgB 877895..878488 (-) 594 WP_001174743.1 XTP/dITP diphosphatase -
  QDY29_RS04335 (QDY29_04335) yggU 878496..878786 (-) 291 WP_001277222.1 DUF167 family protein YggU -
  QDY29_RS04340 (QDY29_04340) yggT 878783..879349 (-) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  QDY29_RS04345 (QDY29_04345) yggS 879367..880071 (-) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  QDY29_RS04350 (QDY29_04350) pilT 880089..881069 (+) 981 WP_282842852.1 type IV pilus twitching motility protein PilT Machinery gene
  QDY29_RS04355 (QDY29_04355) ruvX 881232..881648 (-) 417 WP_000017106.1 Holliday junction resolvase RuvX -
  QDY29_RS04360 (QDY29_04360) yqgE 881648..882211 (-) 564 WP_001384755.1 YqgE/AlgH family protein -
  QDY29_RS04365 (QDY29_04365) gshB 882320..883270 (-) 951 WP_000593273.1 glutathione synthase -
  QDY29_RS04370 (QDY29_04370) rsmE 883283..884014 (-) 732 WP_001222508.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  QDY29_RS04375 (QDY29_04375) endA 884094..884801 (-) 708 WP_000286500.1 deoxyribonuclease I -
  QDY29_RS04380 (QDY29_04380) yggI 884896..885393 (-) 498 WP_000858396.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35854.03 Da        Isoelectric Point: 5.5639

>NTDB_id=743076 QDY29_RS04350 WP_282842852.1 880089..881069(+) (pilT) [Escherichia coli strain ETEC1731]
MNMEEIVALSVKHNVSDLHLCSAWPARWHIRGRMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGCVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSFQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=743076 QDY29_RS04350 WP_282842852.1 880089..881069(+) (pilT) [Escherichia coli strain ETEC1731]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCACATTCGTGGCAGAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAAAATGGCCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
TGCGCCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTTAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACTTTCGCATCGGGGTTGCG
GGCTGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTACGTGACAGTGAGACAATCCGTCTGGCGCTAACGG
CGGCAGAAACCGGGCATCTGGTGCTGGCAACCTTACATACGCGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTACGTAATCAACTGGCAGGTAGTTTACGGGCCGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGATGCGTGGCGCTGTTTGAATTATTGATTAACACTCCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTTTCAGCAGCGA
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

48.93

100

0.491
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.93

100

0.491
  pilT Neisseria meningitidis 8013

48.171

100

0.485
  pilT Neisseria gonorrhoeae MS11

47.866

100

0.482
  pilT Acinetobacter baylyi ADP1

46.177

100

0.463
  pilT Acinetobacter nosocomialis M2

45.872

100

0.46
  pilT Acinetobacter baumannii D1279779

45.872

100

0.46
  pilT Acinetobacter baumannii strain A118

45.872

100

0.46
  pilT Pseudomonas stutzeri DSM 10701

45.872

100

0.46
  pilT Pseudomonas aeruginosa PAK

45.566

100

0.457
  pilT Legionella pneumophila strain ERS1305867

44.648

100

0.448
  pilT Legionella pneumophila strain Lp02

44.648

100

0.448
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.824

97.546

0.408
  pilU Vibrio cholerae strain A1552

38.906

100

0.393
  pilU Pseudomonas stutzeri DSM 10701

37.273

100

0.377