Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   N8I74_RS00990 Genome accession   NZ_CP106753
Coordinates   252563..252973 (-) Length   136 a.a.
NCBI ID   WP_308445869.1    Uniprot ID   -
Organism   Chitiniphilus purpureus strain CD1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 235247..252457 252563..252973 flank 106


Gene organization within MGE regions


Location: 235247..252973
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N8I74_RS00915 (N8I74_00915) - 235247..236095 (+) 849 WP_263125026.1 DUF268 domain-containing protein -
  N8I74_RS00920 (N8I74_00920) - 236028..237371 (+) 1344 WP_263125027.1 flippase -
  N8I74_RS00925 (N8I74_00925) - 237368..238279 (+) 912 WP_263125028.1 alpha-1,2-fucosyltransferase -
  N8I74_RS00930 (N8I74_00930) - 238276..239106 (+) 831 WP_263125029.1 FkbM family methyltransferase -
  N8I74_RS00935 (N8I74_00935) asnB 239111..240967 (+) 1857 WP_263125030.1 asparagine synthase (glutamine-hydrolyzing) -
  N8I74_RS00940 (N8I74_00940) - 240960..241877 (+) 918 WP_263125031.1 sugar transferase -
  N8I74_RS00945 (N8I74_00945) - 241874..243148 (+) 1275 WP_263125032.1 glycosyltransferase -
  N8I74_RS00950 (N8I74_00950) - 243145..243945 (+) 801 WP_263125034.1 bifunctional 2-polyprenyl-6-hydroxyphenol methylase/3-demethylubiquinol 3-O-methyltransferase UbiG -
  N8I74_RS00955 (N8I74_00955) gmd 243974..245092 (+) 1119 WP_263125035.1 GDP-mannose 4,6-dehydratase -
  N8I74_RS00960 (N8I74_00960) - 245105..246070 (+) 966 WP_263125036.1 GDP-L-fucose synthase -
  N8I74_RS00965 (N8I74_00965) - 246048..246824 (+) 777 WP_263125037.1 glycosyltransferase family 2 protein -
  N8I74_RS00970 (N8I74_00970) - 246821..247834 (+) 1014 WP_263125039.1 glycosyltransferase family 4 protein -
  N8I74_RS00975 (N8I74_00975) - 248521..249651 (-) 1131 WP_263125040.1 UDP-N-acetylglucosamine 2-epimerase -
  N8I74_RS00980 (N8I74_00980) - 249670..250557 (-) 888 WP_263125041.1 glycosyltransferase family 2 protein -
  N8I74_RS00985 (N8I74_00985) - 250541..252391 (-) 1851 WP_263125042.1 glycosyltransferase family 39 protein -
  N8I74_RS00990 (N8I74_00990) pilA2 252563..252973 (-) 411 WP_308445869.1 pilin Machinery gene

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14372.62 Da        Isoelectric Point: 10.0024

>NTDB_id=734458 N8I74_RS00990 WP_308445869.1 252563..252973(-) (pilA2) [Chitiniphilus purpureus strain CD1]
MKRMQQGFTLIELMIVVAIIGILAAVAIPAYQDYTARAQVTEGMNLLAGLKTPVSETYADQGVLTIPTTAVRTGRYVQGL
QLNTSTGAITATFKPTGSVNAKVGGKTIVFTYSTNNGRWSCNAASLDPAVRPKSCS

Nucleotide


Download         Length: 411 bp        

>NTDB_id=734458 N8I74_RS00990 WP_308445869.1 252563..252973(-) (pilA2) [Chitiniphilus purpureus strain CD1]
ATGAAAAGAATGCAGCAAGGTTTTACCTTGATCGAGCTGATGATCGTGGTCGCGATTATCGGTATCCTCGCCGCTGTGGC
TATCCCGGCCTACCAGGATTACACCGCCCGCGCCCAGGTGACTGAAGGCATGAATTTGCTTGCAGGTTTGAAGACCCCAG
TCTCGGAAACCTATGCAGATCAAGGAGTTTTGACCATACCGACTACTGCGGTTAGAACGGGTAGATATGTGCAAGGCCTC
CAACTCAATACGAGCACTGGCGCAATTACTGCTACTTTCAAACCGACAGGCAGCGTTAATGCCAAAGTGGGGGGTAAAAC
TATTGTCTTTACTTATTCCACAAACAATGGTCGGTGGAGTTGCAACGCTGCCAGCCTTGATCCTGCAGTCAGGCCAAAGA
GCTGCTCTTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(36-135)

Prokaryotic N-terminal methylation motif (N_methyl)

(2-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

50

98.529

0.493
  pilA2 Legionella pneumophila strain ERS1305867

49.254

98.529

0.485
  pilA/pilAI Pseudomonas stutzeri DSM 10701

44.521

100

0.478
  comP Acinetobacter baylyi ADP1

41.958

100

0.441
  pilE Neisseria gonorrhoeae MS11

44.697

97.059

0.434
  pilE Neisseria gonorrhoeae strain FA1090

43.077

95.588

0.412
  pilA Pseudomonas aeruginosa PAK

36.667

100

0.404
  pilA Vibrio parahaemolyticus RIMD 2210633

39.855

100

0.404
  pilA Acinetobacter baumannii strain A118

39.007

100

0.404
  pilA/pilA1 Eikenella corrodens VA1

46.218

87.5

0.404
  pilA Ralstonia pseudosolanacearum GMI1000

54.167

70.588

0.382
  pilA/pilAII Pseudomonas stutzeri DSM 10701

36.17

100

0.375
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

34.014

100

0.368
  pilA Vibrio cholerae C6706

34.014

100

0.368
  pilA Vibrio cholerae strain A1552

34.014

100

0.368