Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   N5P33_RS22705 Genome accession   NZ_CP104720
Coordinates   4875171..4875623 (-) Length   150 a.a.
NCBI ID   WP_034071565.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain NY4593     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4870171..4880623
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N5P33_RS22685 (N5P33_22685) ampD 4870866..4871432 (-) 567 WP_003104911.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  N5P33_RS22690 (N5P33_22690) - 4871583..4873838 (-) 2256 WP_034071561.1 DUF1631 domain-containing protein -
  N5P33_RS22695 (N5P33_22695) nadC 4874124..4874972 (+) 849 WP_034071563.1 carboxylating nicotinate-nucleotide diphosphorylase -
  N5P33_RS22705 (N5P33_22705) pilA 4875171..4875623 (-) 453 WP_034071565.1 pilin Machinery gene
  N5P33_RS22710 (N5P33_22710) pilB 4875855..4877555 (+) 1701 WP_034071567.1 type IV-A pilus assembly ATPase PilB Machinery gene
  N5P33_RS22715 (N5P33_22715) pilC 4877559..4878779 (+) 1221 WP_034071569.1 type 4a pilus biogenesis protein PilC Machinery gene
  N5P33_RS22720 (N5P33_22720) pilD 4878783..4879655 (+) 873 WP_034071571.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  N5P33_RS22725 (N5P33_22725) coaE 4879652..4880263 (+) 612 WP_034071573.1 dephospho-CoA kinase -
  N5P33_RS22730 (N5P33_22730) yacG 4880260..4880460 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 150 a.a.        Molecular weight: 15743.07 Da        Isoelectric Point: 7.7806

>NTDB_id=731520 N5P33_RS22705 WP_034071565.1 4875171..4875623(-) (pilA) [Pseudomonas aeruginosa strain NY4593]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQNYVARSEGASALATINPLKTTVEESLSRGIAGSKILIGTTASTEDTTYV
GIDEKANKLGTVAVAIKDTGDGTVKFTFATGQSSPKNAGKEITLNRTAEGVWTCTSTQDDMFIPKGCNKP

Nucleotide


Download         Length: 453 bp        

>NTDB_id=731520 N5P33_RS22705 WP_034071565.1 4875171..4875623(-) (pilA) [Pseudomonas aeruginosa strain NY4593]
ATGAAAGCTCAAAAAGGCTTTACCTTGATCGAACTGATGATCGTGGTTGCGATCATCGGTATCCTGGCGGCAATTGCCAT
TCCCCAGTATCAGAACTATGTTGCGCGTTCGGAAGGTGCTTCGGCGCTGGCGACGATCAACCCGCTGAAGACCACTGTTG
AAGAGTCGCTGTCGCGTGGAATTGCTGGTAGCAAAATTTTGATCGGTACTACAGCCTCTACTGAAGATACCACCTATGTT
GGTATTGATGAGAAGGCGAACAAACTCGGTACTGTAGCTGTAGCGATTAAAGACACCGGCGATGGTACTGTAAAATTCAC
TTTTGCGACTGGTCAGTCCAGCCCGAAAAACGCAGGAAAGGAAATTACTTTGAACCGTACTGCGGAAGGTGTATGGACCT
GCACCTCCACTCAGGATGACATGTTCATTCCTAAAGGTTGTAATAAGCCTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

66.447

100

0.673
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

44.375

100

0.473
  pilA Vibrio cholerae strain A1552

44.375

100

0.473
  pilA Vibrio cholerae C6706

44.375

100

0.473
  pilA/pilAI Pseudomonas stutzeri DSM 10701

46.667

100

0.467
  pilA Acinetobacter baumannii strain A118

45.27

98.667

0.447
  pilA Vibrio parahaemolyticus RIMD 2210633

43.243

98.667

0.427
  comP Acinetobacter baylyi ADP1

42

100

0.42
  pilA/pilAII Pseudomonas stutzeri DSM 10701

43.357

95.333

0.413
  pilA2 Legionella pneumophila strain ERS1305867

38.667

100

0.387
  pilA2 Legionella pneumophila str. Paris

38.667

100

0.387
  pilA/pilA1 Eikenella corrodens VA1

39.189

98.667

0.387
  pilA Acinetobacter nosocomialis M2

40.288

92.667

0.373