Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   N5P33_RS22715 Genome accession   NZ_CP104720
Coordinates   4877559..4878779 (+) Length   406 a.a.
NCBI ID   WP_034071569.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain NY4593     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4872559..4883779
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N5P33_RS22695 (N5P33_22695) nadC 4874124..4874972 (+) 849 WP_034071563.1 carboxylating nicotinate-nucleotide diphosphorylase -
  N5P33_RS22705 (N5P33_22705) pilA 4875171..4875623 (-) 453 WP_034071565.1 pilin Machinery gene
  N5P33_RS22710 (N5P33_22710) pilB 4875855..4877555 (+) 1701 WP_034071567.1 type IV-A pilus assembly ATPase PilB Machinery gene
  N5P33_RS22715 (N5P33_22715) pilC 4877559..4878779 (+) 1221 WP_034071569.1 type 4a pilus biogenesis protein PilC Machinery gene
  N5P33_RS22720 (N5P33_22720) pilD 4878783..4879655 (+) 873 WP_034071571.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  N5P33_RS22725 (N5P33_22725) coaE 4879652..4880263 (+) 612 WP_034071573.1 dephospho-CoA kinase -
  N5P33_RS22730 (N5P33_22730) yacG 4880260..4880460 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  N5P33_RS22735 (N5P33_22735) - 4880497..4880706 (-) 210 WP_003094660.1 hypothetical protein -
  N5P33_RS22740 (N5P33_22740) - 4880812..4881501 (-) 690 WP_003103868.1 energy-coupling factor ABC transporter permease -
  N5P33_RS22745 (N5P33_22745) - 4881498..4881968 (-) 471 WP_003094664.1 hypothetical protein -
  N5P33_RS22750 (N5P33_22750) - 4881965..4882390 (-) 426 WP_003103865.1 GNAT family N-acetyltransferase -
  N5P33_RS22755 (N5P33_22755) - 4882523..4883152 (+) 630 WP_003094668.1 DUF1780 domain-containing protein -
  N5P33_RS22760 (N5P33_22760) - 4883149..4883598 (+) 450 WP_003094670.1 MOSC domain-containing protein -

Sequence

Protein


Download         Length: 406 a.a.        Molecular weight: 44358.18 Da        Isoelectric Point: 9.9544

>NTDB_id=731522 N5P33_RS22715 WP_034071569.1 4877559..4878779(+) (pilC) [Pseudomonas aeruginosa strain NY4593]
MAEKALKTSVFIWEGTDKKGAKVKGELTGRNPMLVKAHLRKQGINPLKVRKKGISLLGAGKKVKPMDIALFTRQMATMMG
AGVPLLQSFDIIGEGFDNPNMRKLVDEIKQEVSSGNSLANSLRKKPQYFDELYCNLVDAGEQSGALENLLDRVATYKEKT
ESLKAKIRKAMTYPIAVIIVALIVSAILLIKVVPQFQSVFQGFGAELPAFTQMVVNLSEFLQEWWLAVIVGVGVIGFTFK
ELHKRSKKFRDALDRTILKLPIFGGIVYKSAVARYARTLSTTFAAGVPLVDALDSVSGATGNIVFKNAVSKIKQDVSTGM
QLNFSMRTTSVFPNMAIQMTAIGEESGSLDEMLSKVASYYEEEVDNAVDNLTTLMEPMIMAVLGVLVGGLIVAMYLPIFQ
LGNVVG

Nucleotide


Download         Length: 1221 bp        

>NTDB_id=731522 N5P33_RS22715 WP_034071569.1 4877559..4878779(+) (pilC) [Pseudomonas aeruginosa strain NY4593]
ATGGCGGAAAAAGCGTTAAAAACCAGCGTTTTCATCTGGGAGGGCACCGACAAGAAAGGCGCCAAGGTCAAGGGCGAACT
GACCGGGCGGAATCCCATGCTGGTGAAGGCCCATCTGCGCAAGCAAGGCATCAATCCGCTCAAGGTACGCAAGAAAGGTA
TCTCCCTGCTGGGCGCAGGCAAGAAAGTCAAACCCATGGACATCGCCCTGTTCACCCGGCAGATGGCGACCATGATGGGC
GCTGGCGTTCCCCTCCTGCAATCGTTCGACATCATCGGCGAAGGCTTCGACAATCCCAACATGCGCAAGCTGGTGGATGA
GATCAAGCAAGAAGTGTCCTCGGGCAACAGCCTGGCCAACTCACTGAGAAAGAAACCGCAGTACTTCGACGAACTCTATT
GCAACCTGGTCGATGCCGGCGAGCAATCGGGCGCCTTGGAAAACTTGCTGGATAGAGTCGCGACATACAAGGAAAAAACG
GAATCGTTAAAAGCAAAGATCAGGAAGGCGATGACTTATCCTATCGCCGTCATCATCGTTGCCTTGATCGTCTCCGCCAT
TCTGCTGATCAAGGTCGTTCCGCAGTTCCAATCCGTATTCCAAGGTTTTGGCGCAGAGCTGCCTGCATTCACTCAAATGG
TCGTAAATTTATCCGAGTTCCTACAGGAGTGGTGGCTTGCCGTCATCGTTGGCGTGGGGGTGATTGGCTTTACCTTCAAG
GAACTGCATAAACGCTCGAAAAAATTCCGCGACGCACTCGACAGAACGATCCTCAAACTTCCCATTTTCGGAGGCATCGT
CTACAAATCCGCGGTAGCCCGTTATGCACGGACCTTGTCCACGACCTTCGCCGCGGGTGTTCCCCTGGTCGATGCGCTCG
ACTCCGTCTCCGGAGCGACCGGCAATATCGTGTTCAAGAACGCGGTCAGCAAGATCAAGCAAGACGTTTCCACCGGCATG
CAGCTCAACTTCTCCATGCGCACCACCAGCGTCTTTCCCAACATGGCGATCCAGATGACCGCTATCGGCGAGGAGTCCGG
TTCGCTCGACGAGATGCTGAGCAAAGTCGCCAGCTACTACGAAGAGGAAGTCGACAACGCCGTGGACAACCTCACCACGC
TCATGGAGCCGATGATCATGGCCGTTCTCGGCGTACTGGTTGGCGGTCTGATCGTCGCCATGTACCTTCCGATCTTCCAA
CTCGGCAACGTCGTCGGATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

77.037

99.754

0.768
  pilC Acinetobacter baylyi ADP1

60.591

100

0.606
  pilC Acinetobacter baumannii D1279779

60.494

99.754

0.603
  pilC Legionella pneumophila strain ERS1305867

54.545

97.537

0.532
  pilG Neisseria meningitidis 44/76-A

45.658

99.261

0.453
  pilG Neisseria gonorrhoeae MS11

45.409

99.261

0.451
  pilC Vibrio cholerae strain A1552

42.365

100

0.424
  pilC Vibrio campbellii strain DS40M4

41.604

98.276

0.409
  pilC Thermus thermophilus HB27

36.908

98.768

0.365