Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   N5934_RS15225 Genome accession   NZ_CP104691
Coordinates   3120614..3121594 (+) Length   326 a.a.
NCBI ID   WP_110871555.1    Uniprot ID   -
Organism   Enterobacter hormaechei strain 2022CK-00453     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3115614..3126594
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N5934_RS15185 (N5934_15185) - 3115697..3116023 (+) 327 WP_003862421.1 YggL family protein -
  N5934_RS15190 (N5934_15190) - 3116081..3116797 (+) 717 WP_015571842.1 DUF2884 domain-containing protein -
  N5934_RS15195 (N5934_15195) - 3116881..3117219 (-) 339 WP_015571841.1 endonuclease domain-containing protein -
  N5934_RS15200 (N5934_15200) hemW 3117276..3118424 (-) 1149 WP_110871551.1 radical SAM family heme chaperone HemW -
  N5934_RS15205 (N5934_15205) - 3118417..3119010 (-) 594 WP_045141927.1 XTP/dITP diphosphatase -
  N5934_RS15210 (N5934_15210) yggU 3119014..3119310 (-) 297 WP_023304404.1 DUF167 family protein YggU -
  N5934_RS15215 (N5934_15215) - 3119307..3119873 (-) 567 WP_015571838.1 YggT family protein -
  N5934_RS15220 (N5934_15220) - 3119895..3120596 (-) 702 WP_110871553.1 YggS family pyridoxal phosphate-dependent enzyme -
  N5934_RS15225 (N5934_15225) pilT 3120614..3121594 (+) 981 WP_110871555.1 type IV pilus twitching motility protein PilT Machinery gene
  N5934_RS15230 (N5934_15230) ruvX 3121605..3122021 (-) 417 WP_006811925.1 Holliday junction resolvase RuvX -
  N5934_RS15235 (N5934_15235) - 3122021..3122581 (-) 561 WP_006811924.1 YqgE/AlgH family protein -
  N5934_RS15240 (N5934_15240) gshB 3122656..3123603 (-) 948 WP_015571835.1 glutathione synthase -
  N5934_RS15245 (N5934_15245) rsmE 3123635..3124366 (-) 732 WP_017692692.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  N5934_RS15250 (N5934_15250) endA 3124418..3125125 (-) 708 WP_110871557.1 deoxyribonuclease I -
  N5934_RS15255 (N5934_15255) - 3125220..3125717 (-) 498 WP_110871558.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35709.73 Da        Isoelectric Point: 6.3047

>NTDB_id=731434 N5934_RS15225 WP_110871555.1 3120614..3121594(+) (pilT) [Enterobacter hormaechei strain 2022CK-00453]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRSGRLEPAPFPPPDVEALLKAWLNDEQQGAWWANGQVDFAVTLAGRQRLRG
SAFKHMLGVSIALRLLPLTCPQLSALGVPRAIPELLSNDNGLILVTGATGSGKSTTLAAMVDFLNHQTDGHILTLEDPVE
FMFQSERCLIQQREIGQHSPSFSEALRSALREDPDVILLGELRDNETIRLALTAAETGHLVLATLHTRGAAQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLRQDVQGGRVALYELLVNTSAAANLIREGKTWQLPGIIQTGQQAGMQNFEQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=731434 N5934_RS15225 WP_110871555.1 3120614..3121594(+) (pilT) [Enterobacter hormaechei strain 2022CK-00453]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCGCG
CTGGCGCAGGTCAGGCCGTCTTGAACCTGCGCCGTTTCCGCCCCCGGATGTGGAGGCGTTATTAAAAGCGTGGCTCAACG
ACGAACAGCAGGGCGCATGGTGGGCAAACGGGCAGGTGGATTTTGCCGTTACCCTCGCAGGCCGTCAGCGGCTGCGCGGC
AGTGCGTTTAAGCATATGCTCGGCGTTTCGATTGCGCTGCGGCTGTTGCCGCTGACGTGCCCGCAGCTCTCTGCGTTAGG
TGTGCCGCGAGCGATCCCGGAGCTGCTGTCCAATGACAATGGCCTGATTCTGGTCACCGGCGCCACCGGCAGCGGAAAAT
CGACCACCCTGGCCGCGATGGTCGATTTCCTCAATCACCAGACGGACGGGCATATTCTGACCCTGGAAGATCCGGTGGAG
TTTATGTTCCAGAGCGAACGTTGCCTGATCCAGCAGCGGGAGATAGGCCAGCACAGCCCGTCCTTTTCCGAGGCGCTGCG
CAGTGCGCTGCGTGAAGATCCGGATGTGATTCTGCTTGGTGAGCTGCGTGACAACGAGACGATACGCCTGGCGCTGACGG
CGGCGGAAACCGGCCATCTGGTGTTGGCCACGCTGCACACGCGCGGTGCGGCGCAGGCGATCGAGCGCCTGGTCGACACC
TTTCCGGCGCAGGAGAAAGATCCGGTGCGTAATCAGCTGGCGGGAAGCCTGCGTGCGGTTCTGGCGCAAAAGCTGCGTCA
GGATGTCCAGGGCGGGCGCGTGGCGCTGTATGAGCTGCTGGTGAATACCTCGGCGGCGGCAAATCTGATCCGCGAAGGCA
AAACCTGGCAACTGCCGGGCATTATTCAAACGGGGCAGCAGGCGGGAATGCAGAACTTTGAGCAGAGTCTGGCGGAGCGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(108-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.459

100

0.506
  pilT Vibrio cholerae strain A1552

50.459

100

0.506
  pilT Pseudomonas stutzeri DSM 10701

48.318

100

0.485
  pilT Legionella pneumophila strain Lp02

48.758

98.773

0.482
  pilT Legionella pneumophila strain ERS1305867

48.758

98.773

0.482
  pilT Pseudomonas aeruginosa PAK

47.401

100

0.475
  pilT Neisseria meningitidis 8013

47.256

100

0.475
  pilT Acinetobacter baumannii D1279779

47.095

100

0.472
  pilT Acinetobacter baumannii strain A118

47.095

100

0.472
  pilT Neisseria gonorrhoeae MS11

46.951

100

0.472
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Acinetobacter nosocomialis M2

47.5

98.16

0.466
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.344

99.08

0.429
  pilU Pseudomonas stutzeri DSM 10701

38.182

100

0.387
  pilU Vibrio cholerae strain A1552

37.888

98.773

0.374