Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   N5929_RS17725 Genome accession   NZ_CP104689
Coordinates   3720915..3721895 (-) Length   326 a.a.
NCBI ID   WP_047738304.1    Uniprot ID   -
Organism   Enterobacter hormaechei strain 2020CK-00376     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3715915..3726895
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N5929_RS17695 (N5929_18170) - 3716793..3717290 (+) 498 WP_015571833.1 SprT family zinc-dependent metalloprotease -
  N5929_RS17700 (N5929_18165) endA 3717385..3718092 (+) 708 WP_003860034.1 deoxyribonuclease I -
  N5929_RS17705 (N5929_18160) rsmE 3718144..3718875 (+) 732 WP_017692692.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  N5929_RS17710 (N5929_18155) gshB 3718907..3719854 (+) 948 WP_015571835.1 glutathione synthase -
  N5929_RS17715 (N5929_18150) - 3719929..3720489 (+) 561 WP_006811924.1 YqgE/AlgH family protein -
  N5929_RS17720 (N5929_18145) ruvX 3720489..3720905 (+) 417 WP_006811925.1 Holliday junction resolvase RuvX -
  N5929_RS17725 (N5929_18140) pilT 3720915..3721895 (-) 981 WP_047738304.1 type IV pilus twitching motility protein PilT Machinery gene
  N5929_RS17730 (N5929_18135) - 3721913..3722614 (+) 702 WP_047726777.1 YggS family pyridoxal phosphate-dependent enzyme -
  N5929_RS17735 (N5929_18130) - 3722636..3723202 (+) 567 WP_015571838.1 YggT family protein -
  N5929_RS17740 (N5929_18125) yggU 3723199..3723495 (+) 297 WP_261666011.1 DUF167 family protein YggU -
  N5929_RS17745 (N5929_18120) - 3723499..3724092 (+) 594 WP_015571839.1 XTP/dITP diphosphatase -
  N5929_RS17750 (N5929_18115) hemW 3724085..3725233 (+) 1149 WP_064489706.1 radical SAM family heme chaperone HemW -
  N5929_RS17755 (N5929_18110) - 3725290..3725628 (+) 339 WP_261666010.1 endonuclease domain-containing protein -
  N5929_RS17760 (N5929_18105) - 3725712..3726428 (-) 717 WP_128771910.1 DUF2884 domain-containing protein -
  N5929_RS17765 (N5929_18100) - 3726485..3726811 (-) 327 WP_003862421.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35663.66 Da        Isoelectric Point: 6.2335

>NTDB_id=731416 N5929_RS17725 WP_047738304.1 3720915..3721895(-) (pilT) [Enterobacter hormaechei strain 2020CK-00376]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRSGRLEPAPFPPPDVEALLKAWLNDEQQGAWWANGQVDFAVTLAGRQRLRG
SAFKHMHGVSIALRLLPLTCPQLSALGVPRAIPELLSNDNGLILVTGATGSGKSTTLAAMVDFLNHQTDGHILTLEDPVE
FMYQSERCLIQQREIGQHSPSFAEALRSALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLLQDVQGGRVALYELLVNTSAAANLIREGKTWQLPGIIQTGQQAGMQNFEQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=731416 N5929_RS17725 WP_047738304.1 3720915..3721895(-) (pilT) [Enterobacter hormaechei strain 2020CK-00376]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCGCG
CTGGCGCAGGTCAGGCCGTCTTGAACCTGCGCCGTTTCCGCCCCCGGATGTGGAGGCGTTATTAAAAGCGTGGCTCAACG
ACGAACAGCAGGGCGCATGGTGGGCAAACGGGCAGGTGGATTTTGCCGTTACCCTCGCAGGCCGTCAGCGGCTGCGCGGC
AGTGCGTTTAAGCATATGCACGGCGTTTCGATTGCGCTGCGGCTGTTGCCGCTGACGTGCCCGCAGCTCTCTGCGTTAGG
TGTGCCGCGAGCGATCCCGGAGCTGCTGTCCAATGACAATGGCCTGATTCTGGTCACTGGCGCCACCGGCAGCGGGAAAT
CGACCACCCTGGCCGCGATGGTCGATTTCCTCAATCACCAGACGGACGGGCATATTCTGACCCTGGAAGATCCGGTGGAG
TTTATGTACCAGAGCGAACGTTGCCTGATCCAGCAGCGGGAGATAGGCCAGCACAGCCCGTCCTTTGCCGAGGCGCTGCG
CAGCGCGCTGCGTGAAGATCCGGATGTGATTCTGCTTGGTGAGCTGCGCGACAGCGAGACGATACGCCTGGCGCTGACGG
CGGCGGAAACCGGCCATCTGGTGTTAGCCACGCTGCATACGCGCGGCGCGGCGCAGGCGATCGAGCGCCTGGTCGACACC
TTTCCGGCGCAGGAGAAAGATCCGGTGCGTAATCAGCTGGCGGGAAGCCTGCGTGCGGTCCTGGCGCAGAAGCTACTTCA
GGATGTCCAGGGCGGGCGCGTGGCGCTGTATGAGCTACTGGTGAATACCTCGGCGGCGGCAAACCTGATCCGCGAAGGCA
AAACCTGGCAACTGCCGGGCATTATTCAAACGGGGCAGCAGGCGGGAATGCAGAACTTTGAGCAGAGTCTGGCGGAGCGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(89-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.765

100

0.509
  pilT Vibrio cholerae strain A1552

50.765

100

0.509
  pilT Pseudomonas stutzeri DSM 10701

48.318

100

0.485
  pilT Legionella pneumophila strain Lp02

49.068

98.773

0.485
  pilT Legionella pneumophila strain ERS1305867

49.068

98.773

0.485
  pilT Neisseria meningitidis 8013

47.866

100

0.482
  pilT Neisseria gonorrhoeae MS11

47.561

100

0.479
  pilT Acinetobacter baumannii strain A118

47.401

100

0.475
  pilT Acinetobacter baumannii D1279779

47.401

100

0.475
  pilT Acinetobacter baylyi ADP1

47.095

100

0.472
  pilT Pseudomonas aeruginosa PAK

47.095

100

0.472
  pilT Acinetobacter nosocomialis M2

47.812

98.16

0.469
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.168

98.773

0.426
  pilU Pseudomonas stutzeri DSM 10701

38.182

100

0.387
  pilU Vibrio cholerae strain A1552

38.199

98.773

0.377