Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ATHSA_RS04940 Genome accession   NZ_AP019551
Coordinates   991659..992702 (+) Length   347 a.a.
NCBI ID   WP_161848030.1    Uniprot ID   -
Organism   Athalassotoga saccharophila strain NAS-01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 986659..997702
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ATHSA_RS04930 (ATHSA_1004) - 987733..990981 (-) 3249 WP_161848028.1 LptF/LptG family permease -
  ATHSA_RS04935 (ATHSA_1005) - 990978..991604 (-) 627 WP_161848029.1 PhoU domain-containing protein -
  ATHSA_RS04940 (ATHSA_1006) pilT 991659..992702 (+) 1044 WP_161848030.1 type IV pilus twitching motility protein PilT Machinery gene
  ATHSA_RS04945 (ATHSA_1007) pstB 992764..993513 (-) 750 WP_161848031.1 phosphate ABC transporter ATP-binding protein PstB -
  ATHSA_RS04950 (ATHSA_1008) pstA 993526..994380 (-) 855 WP_161848032.1 phosphate ABC transporter permease PstA -
  ATHSA_RS04955 (ATHSA_1009) pstC 994380..995246 (-) 867 WP_161848033.1 phosphate ABC transporter permease subunit PstC -
  ATHSA_RS04960 (ATHSA_1010) - 995253..996068 (-) 816 WP_161848034.1 PstS family phosphate ABC transporter substrate-binding protein -
  ATHSA_RS04965 (ATHSA_1011) - 996384..997388 (+) 1005 WP_197739106.1 3-oxoacyl-ACP synthase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38650.73 Da        Isoelectric Point: 6.9289

>NTDB_id=72919 ATHSA_RS04940 WP_161848030.1 991659..992702(+) (pilT) [Athalassotoga saccharophila strain NAS-01]
MKGLNITELLIEAYKMNASDVHLSVDMPPVFRIYGDLVPQIKYEPYKSEELLKLISELLKSVNIPFNKKEIDFSFGLENV
ARVRANVFLERGNIAAAFRIITYKIRNFNELGLPPILSEFANHASGIVLIAGPTGSGKSTTLAAMIDKINQERPVHIITI
EDPIEYIFQNKQALIHQRQLGNDTDSFADGLKYALRQDPDVILVGEMRDIETMRLALTAAETGHLVFSTIHTNSAGSAPE
RIIDVFPEHQQKQIAIQLSNTLVGIAYQRLLPSANGKGYVPIVEILVGTVAVRNLIRENKLHQIDSMIETGSKFGMITFD
EALKRAISLNKIKKNDAILYAKDPSSL

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=72919 ATHSA_RS04940 WP_161848030.1 991659..992702(+) (pilT) [Athalassotoga saccharophila strain NAS-01]
ATGAAAGGCCTTAACATAACTGAATTGTTGATCGAAGCGTACAAAATGAACGCTTCAGATGTCCATTTGAGTGTTGATAT
GCCACCGGTCTTTAGAATTTACGGTGATCTTGTTCCACAGATAAAGTATGAACCTTACAAATCAGAAGAACTCTTAAAGT
TGATAAGTGAACTTTTAAAATCTGTCAACATTCCATTCAACAAAAAAGAAATTGATTTTTCATTTGGGCTTGAAAATGTT
GCGAGGGTGAGGGCAAATGTTTTTTTGGAAAGAGGGAATATAGCGGCAGCCTTCAGAATAATAACCTACAAGATAAGGAA
TTTCAACGAACTCGGTCTTCCTCCAATACTGTCCGAGTTTGCAAATCACGCAAGCGGAATCGTTCTAATAGCAGGGCCGA
CGGGATCTGGAAAATCAACGACTCTTGCGGCGATGATAGACAAAATAAATCAGGAAAGGCCAGTCCACATAATCACTATA
GAGGATCCTATCGAATACATCTTTCAGAACAAACAGGCATTGATACATCAAAGACAACTTGGAAATGATACTGATTCCTT
TGCAGATGGTCTAAAGTATGCCCTTAGGCAGGATCCAGACGTTATCCTCGTTGGAGAAATGAGAGACATTGAGACGATGA
GGCTTGCCTTGACTGCCGCGGAAACGGGTCACCTTGTCTTTTCAACAATACACACCAATTCGGCGGGATCAGCGCCGGAA
AGGATAATAGATGTTTTCCCCGAACATCAGCAAAAGCAGATCGCAATCCAACTTTCAAATACTCTTGTAGGTATAGCGTA
CCAGAGGCTTTTACCTTCGGCGAATGGAAAGGGATACGTTCCGATAGTCGAAATTCTCGTCGGAACTGTCGCCGTTAGAA
ATTTAATACGCGAAAACAAGTTGCATCAAATAGATTCCATGATAGAGACTGGAAGTAAGTTTGGAATGATAACCTTTGAC
GAAGCTCTAAAGAGGGCGATATCTTTGAATAAAATAAAAAAGAACGACGCGATCCTTTACGCAAAGGATCCATCGAGCCT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.547

99.135

0.481
  pilT Acinetobacter nosocomialis M2

47.399

99.712

0.473
  pilT Acinetobacter baumannii D1279779

47.11

99.712

0.47
  pilT Acinetobacter baumannii strain A118

47.11

99.712

0.47
  pilT Neisseria meningitidis 8013

46.398

100

0.464
  pilT Acinetobacter baylyi ADP1

46.243

99.712

0.461
  pilT Neisseria gonorrhoeae MS11

45.954

99.712

0.458
  pilT Legionella pneumophila strain Lp02

47.321

96.83

0.458
  pilT Legionella pneumophila strain ERS1305867

47.321

96.83

0.458
  pilT Pseudomonas aeruginosa PAK

44.22

99.712

0.441
  pilT Vibrio cholerae strain A1552

46.789

94.236

0.441
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

46.789

94.236

0.441
  pilT Pseudomonas stutzeri DSM 10701

43.931

99.712

0.438
  pilU Acinetobacter baylyi ADP1

37.322

100

0.378
  pilU Vibrio cholerae strain A1552

38.066

95.389

0.363

Multiple sequence alignment