Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   K7563_RS16040 Genome accession   NZ_CP104289
Coordinates   3479288..3479698 (-) Length   136 a.a.
NCBI ID   WP_227837948.1    Uniprot ID   -
Organism   Stenotrophomonas maltophilia strain ACYCd.9D     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 3480054..3489823 3479288..3479698 flank 356


Gene organization within MGE regions


Location: 3479288..3489823
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K7563_RS16040 (K7563_16040) pilA 3479288..3479698 (-) 411 WP_227837948.1 pilin Machinery gene
  K7563_RS16045 (K7563_16045) pilC 3480054..3481313 (+) 1260 WP_227837946.1 type II secretion system F family protein Machinery gene
  K7563_RS16050 (K7563_16050) - 3481322..3482185 (+) 864 WP_005418626.1 A24 family peptidase -
  K7563_RS16055 (K7563_16055) coaE 3482197..3482808 (+) 612 WP_227837944.1 dephospho-CoA kinase -
  K7563_RS16060 (K7563_16060) - 3482982..3483368 (-) 387 WP_065196692.1 hypothetical protein -
  K7563_RS16065 (K7563_16065) - 3483904..3485274 (-) 1371 WP_069139309.1 HAMP domain-containing sensor histidine kinase -
  K7563_RS16070 (K7563_16070) - 3485237..3485914 (-) 678 WP_005410804.1 response regulator transcription factor -
  K7563_RS16075 (K7563_16075) - 3486076..3486537 (-) 462 WP_065185267.1 hypothetical protein -
  K7563_RS16080 (K7563_16080) - 3486653..3487123 (-) 471 WP_072167638.1 hypothetical protein -
  K7563_RS16085 (K7563_16085) - 3487218..3487697 (-) 480 WP_151354682.1 hypothetical protein -
  K7563_RS16090 (K7563_16090) rimK 3487956..3488834 (-) 879 WP_057494737.1 30S ribosomal protein S6--L-glutamate ligase -
  K7563_RS16095 (K7563_16095) - 3488932..3489174 (-) 243 WP_061479300.1 hypothetical protein -
  K7563_RS16100 (K7563_16100) - 3489320..3489823 (+) 504 WP_227837942.1 hypothetical protein -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 13985.19 Da        Isoelectric Point: 8.1012

>NTDB_id=728316 K7563_RS16040 WP_227837948.1 3479288..3479698(-) (pilA) [Stenotrophomonas maltophilia strain ACYCd.9D]
MKNQKGFTLIELMIVVAIIAILAAIAIPQYNDYTARAQLSEAFTLASGLKTPIAEAYAQDNAAANSCKVPANSVVTGKYV
EKVEASGDCAVVATMKSAGVNNKVSGSTVTLTFAPATGAWTCATTAPAEVKPKACI

Nucleotide


Download         Length: 411 bp        

>NTDB_id=728316 K7563_RS16040 WP_227837948.1 3479288..3479698(-) (pilA) [Stenotrophomonas maltophilia strain ACYCd.9D]
ATGAAGAACCAGAAGGGCTTCACCCTTATCGAACTGATGATCGTTGTCGCGATCATCGCCATCCTGGCCGCGATCGCGAT
CCCGCAGTACAACGATTACACCGCTCGCGCGCAGCTGTCGGAAGCGTTCACCCTGGCGAGCGGTCTCAAGACCCCGATCG
CTGAAGCTTATGCTCAGGACAACGCCGCAGCTAATTCGTGCAAGGTGCCGGCGAACTCGGTCGTGACCGGCAAGTATGTT
GAAAAGGTTGAGGCGAGCGGTGATTGCGCGGTTGTCGCAACCATGAAGTCCGCCGGTGTGAACAACAAGGTGTCTGGTTC
GACCGTGACCCTGACCTTTGCTCCGGCAACCGGCGCATGGACCTGTGCGACCACCGCCCCGGCCGAGGTCAAGCCGAAGG
CTTGCATCTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-135)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter nosocomialis M2

45.578

100

0.493
  comP Acinetobacter baylyi ADP1

41.611

100

0.456
  pilE Neisseria gonorrhoeae strain FA1090

48.062

94.853

0.456
  pilA Pseudomonas aeruginosa PAK

40.667

100

0.449
  pilE Neisseria gonorrhoeae MS11

46.875

94.118

0.441
  pilA Acinetobacter baumannii strain A118

42.143

100

0.434
  pilA2 Legionella pneumophila str. Paris

40.411

100

0.434
  pilA/pilA1 Eikenella corrodens VA1

42.647

100

0.426
  pilA2 Legionella pneumophila strain ERS1305867

38.889

100

0.412
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

36.667

100

0.404
  pilA Vibrio cholerae strain A1552

36.667

100

0.404
  pilA Vibrio cholerae C6706

36.667

100

0.404
  pilA/pilAI Pseudomonas stutzeri DSM 10701

39.007

100

0.404
  pilA Vibrio parahaemolyticus RIMD 2210633

38.06

98.529

0.375