Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   N2K86_RS18210 Genome accession   NZ_CP104285
Coordinates   3864451..3865431 (-) Length   326 a.a.
NCBI ID   WP_260659532.1    Uniprot ID   -
Organism   Enterobacter mori strain JT.W3M11     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3859451..3870431
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N2K86_RS18180 (N2K86_18180) - 3860340..3860837 (+) 498 WP_061097110.1 SprT family zinc-dependent metalloprotease -
  N2K86_RS18185 (N2K86_18185) endA 3860932..3861639 (+) 708 WP_260659531.1 deoxyribonuclease I -
  N2K86_RS18190 (N2K86_18190) rsmE 3861691..3862422 (+) 732 WP_260661722.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  N2K86_RS18195 (N2K86_18195) gshB 3862442..3863389 (+) 948 WP_041908326.1 glutathione synthase -
  N2K86_RS18200 (N2K86_18200) - 3863464..3864024 (+) 561 WP_010435456.1 YqgE/AlgH family protein -
  N2K86_RS18205 (N2K86_18205) ruvX 3864024..3864440 (+) 417 WP_006811925.1 Holliday junction resolvase RuvX -
  N2K86_RS18210 (N2K86_18210) pilT 3864451..3865431 (-) 981 WP_260659532.1 type IV pilus twitching motility protein PilT Machinery gene
  N2K86_RS18215 (N2K86_18215) - 3865449..3866150 (+) 702 WP_260659533.1 YggS family pyridoxal phosphate-dependent enzyme -
  N2K86_RS18220 (N2K86_18220) - 3866172..3866738 (+) 567 WP_010435467.1 YggT family protein -
  N2K86_RS18225 (N2K86_18225) yggU 3866735..3867031 (+) 297 WP_023333349.1 DUF167 family protein YggU -
  N2K86_RS18230 (N2K86_18230) - 3867035..3867628 (+) 594 WP_260659534.1 XTP/dITP diphosphatase -
  N2K86_RS18235 (N2K86_18235) hemW 3867621..3868763 (+) 1143 WP_260659535.1 radical SAM family heme chaperone HemW -
  N2K86_RS18240 (N2K86_18240) - 3868827..3869189 (+) 363 WP_260659536.1 endonuclease domain-containing protein -
  N2K86_RS18245 (N2K86_18245) - 3869357..3870073 (-) 717 WP_126815894.1 DUF2884 domain-containing protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35665.75 Da        Isoelectric Point: 6.3407

>NTDB_id=728258 N2K86_RS18210 WP_260659532.1 3864451..3865431(-) (pilT) [Enterobacter mori strain JT.W3M11]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRLGRLEPAPFPAPDVEALLKTWLNDEQQGAWWANGQVDFAVTLTGNQRLRA
SAFTHMKGVSITLRLLPLACPQLSALGVPRAIPELLSSDSGLILVTGATGSGKSTTLAAMVDFLNHHTDGHILTLEDPVE
FMYQSERCLIQQREIGQHSLSFAEALRSALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGASQAIERLVDT
FPAHEKDPVRNQLAGSLRAVLAQKLLKDVQGGRVALYELLVNTPAAANLIREGKTWQLPGIIQTGQQAGMQNFEQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=728258 N2K86_RS18210 WP_260659532.1 3864451..3865431(-) (pilT) [Enterobacter mori strain JT.W3M11]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCACCGCCGCG
CTGGCGCAGATTAGGCCGCCTTGAACCGGCTCCCTTTCCTGCCCCCGACGTGGAGGCGCTATTAAAAACCTGGCTCAACG
ATGAACAGCAGGGCGCGTGGTGGGCAAACGGACAGGTAGACTTTGCCGTCACGCTCACAGGCAATCAGCGTCTTCGCGCC
AGTGCGTTTACGCATATGAAGGGTGTGTCGATCACCTTGCGGCTGTTGCCGCTCGCGTGCCCGCAGCTTTCTGCACTGGG
TGTGCCCAGGGCTATCCCGGAGCTGCTCTCCAGCGACAGCGGGTTGATTCTGGTGACGGGCGCGACCGGTAGCGGAAAGT
CCACAACGCTTGCAGCAATGGTGGATTTTCTCAATCATCACACTGACGGCCATATTCTGACGCTCGAAGATCCTGTGGAG
TTTATGTACCAGAGCGAACGCTGCCTGATACAGCAGCGGGAGATTGGTCAGCACAGTCTCTCCTTTGCCGAGGCGCTGCG
CAGTGCGCTGCGCGAAGATCCGGATGTCATCCTGCTGGGAGAGTTGCGCGACAGCGAAACGATCCGCCTGGCGCTGACGG
CGGCAGAAACTGGCCATTTGGTGCTGGCAACGCTGCACACGCGCGGCGCTTCGCAGGCGATAGAACGGCTGGTGGATACC
TTCCCGGCGCATGAGAAAGATCCTGTGCGTAACCAGCTGGCAGGCAGCCTGCGCGCTGTACTGGCACAAAAATTGCTTAA
GGATGTACAGGGCGGGCGCGTGGCGCTGTATGAACTGCTGGTGAACACCCCGGCGGCGGCGAATCTCATCCGCGAAGGGA
AAACGTGGCAATTACCCGGCATCATACAAACCGGTCAGCAGGCGGGAATGCAGAACTTTGAGCAGAGTCTGGCGGAAAGG
CGGGCGCAGGGGCGGCTATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(73-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

51.376

100

0.515
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.376

100

0.515
  pilT Pseudomonas stutzeri DSM 10701

49.235

100

0.494
  pilT Neisseria meningitidis 8013

48.78

100

0.491
  pilT Neisseria gonorrhoeae MS11

48.476

100

0.488
  pilT Acinetobacter baylyi ADP1

48.318

100

0.485
  pilT Legionella pneumophila strain Lp02

49.068

98.773

0.485
  pilT Legionella pneumophila strain ERS1305867

49.068

98.773

0.485
  pilT Acinetobacter baumannii D1279779

48.012

100

0.482
  pilT Acinetobacter baumannii strain A118

48.012

100

0.482
  pilT Pseudomonas aeruginosa PAK

47.706

100

0.479
  pilT Acinetobacter nosocomialis M2

48.438

98.16

0.475
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.168

98.773

0.426
  pilU Pseudomonas stutzeri DSM 10701

38.182

100

0.387
  pilU Vibrio cholerae strain A1552

38.509

98.773

0.38