Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   N2K85_RS17485 Genome accession   NZ_CP104284
Coordinates   3729285..3730265 (-) Length   326 a.a.
NCBI ID   WP_063659199.1    Uniprot ID   -
Organism   Enterobacter hormaechei subsp. steigerwaltii strain JT.WLB1A     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3724285..3735265
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N2K85_RS17455 (N2K85_17455) - 3725163..3725660 (+) 498 WP_063160924.1 SprT family zinc-dependent metalloprotease -
  N2K85_RS17460 (N2K85_17460) endA 3725755..3726462 (+) 708 WP_003860034.1 deoxyribonuclease I -
  N2K85_RS17465 (N2K85_17465) rsmE 3726514..3727245 (+) 732 WP_017382923.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  N2K85_RS17470 (N2K85_17470) gshB 3727277..3728224 (+) 948 WP_015571835.1 glutathione synthase -
  N2K85_RS17475 (N2K85_17475) - 3728299..3728859 (+) 561 WP_006811924.1 YqgE/AlgH family protein -
  N2K85_RS17480 (N2K85_17480) ruvX 3728859..3729275 (+) 417 WP_006811925.1 Holliday junction resolvase RuvX -
  N2K85_RS17485 (N2K85_17485) pilT 3729285..3730265 (-) 981 WP_063659199.1 type IV pilus twitching motility protein PilT Machinery gene
  N2K85_RS17490 (N2K85_17490) - 3730283..3730984 (+) 702 WP_045347340.1 YggS family pyridoxal phosphate-dependent enzyme -
  N2K85_RS17495 (N2K85_17495) - 3731006..3731572 (+) 567 WP_015571838.1 YggT family protein -
  N2K85_RS17500 (N2K85_17500) yggU 3731569..3731865 (+) 297 WP_023304404.1 DUF167 family protein YggU -
  N2K85_RS17505 (N2K85_17505) - 3731869..3732462 (+) 594 WP_045327517.1 XTP/dITP diphosphatase -
  N2K85_RS17510 (N2K85_17510) hemW 3732455..3733603 (+) 1149 WP_260673222.1 radical SAM family heme chaperone HemW -
  N2K85_RS17515 (N2K85_17515) - 3733660..3733998 (+) 339 WP_047057506.1 endonuclease domain-containing protein -
  N2K85_RS17520 (N2K85_17520) - 3734082..3734798 (-) 717 WP_015571842.1 DUF2884 domain-containing protein -
  N2K85_RS17525 (N2K85_17525) - 3734855..3735181 (-) 327 WP_003862421.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35709.75 Da        Isoelectric Point: 6.4131

>NTDB_id=728235 N2K85_RS17485 WP_063659199.1 3729285..3730265(-) (pilT) [Enterobacter hormaechei subsp. steigerwaltii strain JT.WLB1A]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRSGRLEPAPFPPPDVEALLKAWLNDEQQGAWWANGQVDFAVTLAGRQRLRG
SAFKHMLGVSIALRLLPLTCPQLSALGVPRAIPELLSNDNGLILVTGATGSGKSTTLAAMVDFLNHHTDGHILTLEDPVE
FMYQSERCLIQQREIGQHSPSFAEALRSAMREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLRQDVQGGRVALYELLVNTSAAANLIREGKTWQLPGIIQTGQQAGMQNFEQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=728235 N2K85_RS17485 WP_063659199.1 3729285..3730265(-) (pilT) [Enterobacter hormaechei subsp. steigerwaltii strain JT.WLB1A]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCGCG
CTGGCGCAGGTCAGGCCGTCTTGAACCTGCGCCGTTTCCGCCCCCGGATGTGGAGGCGTTATTAAAAGCGTGGCTCAACG
ACGAACAGCAGGGCGCATGGTGGGCAAACGGGCAAGTGGATTTTGCCGTTACCCTCGCAGGCCGTCAGCGGCTGCGCGGC
AGTGCGTTTAAGCATATGCTCGGCGTTTCGATTGCGCTGCGGCTGTTGCCGCTGACGTGCCCGCAGCTCTCTGCGCTAGG
TGTGCCGCGAGCGATCCCGGAACTACTGTCCAATGACAATGGCCTGATTCTGGTCACCGGCGCCACCGGCAGTGGGAAAT
CGACCACCCTGGCCGCTATGGTCGATTTCCTCAATCACCATACGGACGGGCATATTCTGACCCTGGAAGATCCGGTGGAG
TTTATGTACCAGAGCGAACGTTGCCTGATCCAGCAGCGGGAGATAGGCCAGCACAGCCCGTCCTTTGCCGAGGCGCTGCG
CAGCGCGATGCGTGAAGATCCGGATGTGATTCTGCTTGGTGAGCTGCGCGACAGCGAGACGATACGCCTGGCGCTGACGG
CGGCGGAAACCGGCCATCTGGTGTTAGCCACGCTGCACACGCGCGGCGCGGCGCAGGCGATCGAGCGCCTGGTCGACACC
TTTCCGGCGCAGGAGAAAGATCCGGTGCGTAATCAGCTGGCGGGAAGCCTGCGTGCGGTCCTGGCGCAGAAGCTACGTCA
GGATGTCCAGGGCGGGCGCGTGGCGCTGTATGAGCTACTGGTGAATACCTCGGCGGCGGCAAACCTGATCCGCGAAGGCA
AAACCTGGCAACTGCCGGGCATTATTCAAACGGGGCAGCAGGCGGGAATGCAGAACTTTGAGCAGAGTCTGGCGGAGCGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(108-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

50.459

100

0.506
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.459

100

0.506
  pilT Legionella pneumophila strain Lp02

48.447

98.773

0.479
  pilT Legionella pneumophila strain ERS1305867

48.447

98.773

0.479
  pilT Pseudomonas stutzeri DSM 10701

47.706

100

0.479
  pilT Neisseria meningitidis 8013

47.256

100

0.475
  pilT Neisseria gonorrhoeae MS11

46.951

100

0.472
  pilT Acinetobacter baumannii strain A118

46.789

100

0.469
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Acinetobacter baumannii D1279779

46.789

100

0.469
  pilT Pseudomonas aeruginosa PAK

46.789

100

0.469
  pilT Acinetobacter nosocomialis M2

47.188

98.16

0.463
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.034

99.08

0.426
  pilU Pseudomonas stutzeri DSM 10701

37.879

100

0.383
  pilU Vibrio cholerae strain A1552

37.888

98.773

0.374