Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   N0D28_RS02855 Genome accession   NZ_CP104213
Coordinates   549366..550598 (+) Length   410 a.a.
NCBI ID   WP_260560889.1    Uniprot ID   -
Organism   Deinococcus rubellus strain Ant6     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 544366..555598
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N0D28_RS02835 (N0D28_02835) trmH 544508..545191 (-) 684 WP_260560885.1 tRNA (guanosine(18)-2'-O)-methyltransferase TrmH -
  N0D28_RS02840 (N0D28_02840) pgeF 545312..546052 (+) 741 WP_260560886.1 peptidoglycan editing factor PgeF -
  N0D28_RS02845 (N0D28_02845) - 546054..546647 (+) 594 WP_260560887.1 YqeG family HAD IIIA-type phosphatase -
  N0D28_RS02850 (N0D28_02850) pilB 546644..549307 (+) 2664 WP_260560888.1 ATPase, T2SS/T4P/T4SS family Machinery gene
  N0D28_RS02855 (N0D28_02855) pilT 549366..550598 (+) 1233 WP_260560889.1 type IV pilus twitching motility protein PilT Machinery gene
  N0D28_RS02860 (N0D28_02860) - 550937..551677 (-) 741 WP_260560890.1 metallophosphoesterase -
  N0D28_RS02865 (N0D28_02865) - 551836..552942 (+) 1107 WP_260560891.1 hypothetical protein -
  N0D28_RS02870 (N0D28_02870) - 553034..554275 (+) 1242 WP_260560892.1 MFS transporter -
  N0D28_RS02875 (N0D28_02875) - 554272..555597 (-) 1326 WP_260560893.1 hemolysin family protein -

Sequence

Protein


Download         Length: 410 a.a.        Molecular weight: 44595.87 Da        Isoelectric Point: 7.5591

>NTDB_id=728084 N0D28_RS02855 WP_260560889.1 549366..550598(+) (pilT) [Deinococcus rubellus strain Ant6]
MTTFGPDITDILRIAAEKDASDVILTVGLPPQFKLSGVYGHQDMAVLGATDTRKLMYSMMNDKQQRQFEDKRELDFSFAL
GEQARFRVNAFMQRGYVGGVMRLIPTKIKSVQDLGLPQSVVEIANAPRGLVLVTGPTGSGKSTTLAAMLDYINVNKKLHI
VTIEDPIEFMHQHKQSIVNQREVGADTYGFDNALRAVLRQAPDVILVGEMRDYETIKAAVTAAETGHLVMGTLHTNSAPE
SIDRIVDVFPEEQQAQIRVQLANNLVAVMTQQLIPRLDGGRVLAYELLVATPAVRALIREGKTYQIVSVMQTSSREGMVT
MDAFLAGLYRRRIISHDAGLERSVDPKEFTRLASDPGGGNPNAVPSAGLSNSYNGPASPAAGRSNGGREAVPSAYGAPET
RSGDKPFGRR

Nucleotide


Download         Length: 1233 bp        

>NTDB_id=728084 N0D28_RS02855 WP_260560889.1 549366..550598(+) (pilT) [Deinococcus rubellus strain Ant6]
ATGACAACGTTCGGCCCCGACATCACCGACATTCTGCGGATCGCCGCCGAAAAAGACGCCTCCGACGTGATCCTGACCGT
CGGCCTGCCGCCTCAGTTCAAGCTCTCGGGCGTCTACGGGCACCAGGACATGGCAGTGCTGGGCGCAACCGACACCCGCA
AGCTGATGTACAGCATGATGAACGACAAGCAGCAGCGCCAGTTCGAGGACAAGCGCGAGCTCGACTTCTCCTTTGCGCTG
GGCGAGCAGGCCCGCTTCCGTGTCAACGCTTTCATGCAGCGCGGCTACGTCGGCGGCGTGATGCGTCTGATCCCCACCAA
GATCAAGAGCGTGCAGGACCTCGGCCTGCCGCAGAGCGTCGTGGAGATCGCCAACGCCCCGCGCGGCCTGGTGCTGGTGA
CCGGCCCCACCGGCTCGGGCAAATCCACGACCCTGGCGGCCATGCTCGACTACATCAACGTCAACAAGAAGCTGCATATC
GTCACCATCGAAGACCCGATCGAGTTCATGCACCAGCACAAGCAGAGTATCGTCAACCAGCGCGAGGTGGGGGCCGATAC
CTACGGCTTTGACAATGCCCTGCGCGCGGTGCTGCGGCAGGCCCCTGACGTGATCCTGGTGGGCGAAATGCGCGACTACG
AGACCATCAAGGCCGCTGTGACCGCCGCTGAAACCGGGCACCTGGTGATGGGAACGCTGCACACCAACTCGGCCCCCGAA
TCGATTGACCGCATCGTGGACGTGTTCCCCGAAGAACAGCAGGCCCAGATCCGGGTGCAGCTCGCCAACAACCTGGTGGC
GGTCATGACCCAGCAGCTCATTCCGCGCCTCGACGGGGGCCGGGTGCTGGCCTACGAACTGCTGGTCGCCACCCCGGCGG
TGCGTGCCCTGATCCGCGAGGGCAAGACCTACCAGATCGTCTCGGTGATGCAGACCAGTTCGCGCGAGGGCATGGTGACG
ATGGACGCCTTCCTGGCGGGGCTGTACCGCCGCCGGATCATCAGCCACGACGCGGGTCTGGAGCGGTCCGTGGACCCCAA
GGAATTCACCCGGCTGGCCAGCGATCCCGGCGGCGGCAACCCCAACGCGGTGCCGAGCGCGGGCCTGAGCAACTCCTACA
ATGGCCCGGCCAGCCCAGCGGCGGGCCGCAGCAACGGTGGGCGTGAGGCGGTGCCCAGCGCTTACGGCGCTCCCGAGACC
CGCAGCGGCGACAAACCGTTCGGGCGGCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(52-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

75.781

93.659

0.71
  pilT Pseudomonas stutzeri DSM 10701

52.187

83.659

0.437
  pilT Acinetobacter nosocomialis M2

51.895

83.659

0.434
  pilT Acinetobacter baumannii D1279779

51.895

83.659

0.434
  pilT Acinetobacter baumannii strain A118

51.895

83.659

0.434
  pilT Pseudomonas aeruginosa PAK

51.895

83.659

0.434
  pilT Legionella pneumophila strain Lp02

50

83.415

0.417
  pilT Legionella pneumophila strain ERS1305867

50

83.415

0.417
  pilT Neisseria meningitidis 8013

49.565

84.146

0.417
  pilT Acinetobacter baylyi ADP1

49.854

83.659

0.417
  pilT Neisseria gonorrhoeae MS11

49.128

83.902

0.412
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.39

80

0.395
  pilT Vibrio cholerae strain A1552

49.39

80

0.395
  pilU Pseudomonas stutzeri DSM 10701

43.132

88.78

0.383