Detailed information NTG0728083

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: N0D28_RS02850
• Genome accession: NZ_CP104213
• Coordinates: 546644..549307 (+)
• Length: 887 a.a.
• NCBI ID: WP_260560888.1
• Uniprot ID: -
• Organism: Deinococcus rubellus strain Ant6
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 541644..554307

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
N0D28_RS02825 (N0D28_02825)	-	542275..543633 (-)	1359	WP_260560883.1	TerD family protein	-
N0D28_RS02830 (N0D28_02830)	-	543742..544317 (-)	576	WP_260560884.1	TerD family protein	-
N0D28_RS02835 (N0D28_02835)	trmH	544508..545191 (-)	684	WP_260560885.1	tRNA (guanosine(18)-2'-O)-methyltransferase TrmH	-
N0D28_RS02840 (N0D28_02840)	pgeF	545312..546052 (+)	741	WP_260560886.1	peptidoglycan editing factor PgeF	-
N0D28_RS02845 (N0D28_02845)	-	546054..546647 (+)	594	WP_260560887.1	YqeG family HAD IIIA-type phosphatase	-
N0D28_RS02850 (N0D28_02850)	pilB	546644..549307 (+)	2664	WP_260560888.1	ATPase, T2SS/T4P/T4SS family	Machinery gene
N0D28_RS02855 (N0D28_02855)	pilT	549366..550598 (+)	1233	WP_260560889.1	type IV pilus twitching motility protein PilT	Machinery gene
N0D28_RS02860 (N0D28_02860)	-	550937..551677 (-)	741	WP_260560890.1	metallophosphoesterase	-
N0D28_RS02865 (N0D28_02865)	-	551836..552942 (+)	1107	WP_260560891.1	hypothetical protein	-
N0D28_RS02870 (N0D28_02870)	-	553034..554275 (+)	1242	WP_260560892.1	MFS transporter	-

Sequence

Protein

• Length: 887 a.a.
• Molecular weight: 97001.93 Da
• Isoelectric Point: 5.0736

>NTDB_id=728083 N0D28_RS02850 WP_260560888.1 546644..549307(+) (pilB) [Deinococcus rubellus strain Ant6]
MTLSIGDRRLGAILLEQGYVNDTDLQKALDRHVEVGGRLADILIDGGVVGEKRIARALEEAFGLPLVDLTALTPDPHAIA
SIPASAAVQAQGVPFALENDTLRVAFVDALNNVAIETMEDESGYIIEPYQALREQVAWAIASFYPELGLPIPALSESRAS
GLSLLGQRLVSRGYVSEEQVLSALEQQRSSGVSLGTVLLGSKLLTEDQLYETLAEQEDSQYVRDTREYQPTEEVLGLLLR
ADALRLASVPVSQDGNVVSVITGDLRRREDIEALIGHKVRLMLARPSDVDTLIDRAYPQKGRLGEAMVQQGTLSRAQLRE
ALQVQARQGKVKPLGEVIMDLGFAGSEEIDAALVKQNAGGGRLEDTLVQSGKLSPEMLARSLAAQLGYEFVDPVTSPPDT
KVALMIPEATARRYTVVPMRLQGESLVIAMKDPRNVFALDDLKLITGREILPAVMAEKDIVRLIERYFGDKDMAKLNEEL
AKSSRQKDKEKESVDLSSLDDNAVVRVIDGLIREAALQDASDIHIEPTEHSVRVRYRIDGSLREHMELPRGAAQSMLARI
KIMGNLDIAERRVPQDGRVRFKKGSIDLDLRLSTLPTVYGEKAVMRLLQKASNIPEVEQLGFSEHNFQRFLDVIEKPNGI
FLVTGPTGSGKSFSSFSTLKRIARPDKNTTTIEDPIEYEIPGIIQSQVNTVAGFTFARALRAFLRQDPDIIFVGEVRDTE
TAKIATEAALTGHLVLATLHTNDAPGAITRLEEMGVEPFNISASVIGVLAQRLVRKVCTECKRPTNADPEVLRRLGIGER
ELRGANLQRGAGCPRCGGTGYKGRMGIHELMVVDDPIRRAIGTHQTAAEIREVATTQSGMKTLRQDGVEKALIGLTTLEE
VLAVTSN

Nucleotide

• Length: 2664 bp

>NTDB_id=728083 N0D28_RS02850 WP_260560888.1 546644..549307(+) (pilB) [Deinococcus rubellus strain Ant6]
GTGACCCTTTCGATTGGAGACCGCCGCCTCGGGGCCATCTTGCTGGAGCAGGGCTACGTCAACGACACTGACCTGCAAAA
AGCGCTCGACCGCCACGTCGAGGTGGGAGGCCGCCTGGCCGATATCCTGATCGACGGCGGCGTCGTGGGCGAGAAGCGCA
TCGCCCGCGCCCTGGAAGAAGCCTTCGGGCTGCCGCTGGTCGACCTCACCGCCCTGACGCCCGATCCGCATGCCATTGCC
AGCATTCCGGCCTCGGCAGCGGTGCAGGCCCAGGGGGTGCCGTTCGCGCTGGAAAACGACACCCTGCGGGTGGCCTTCGT
GGACGCCCTCAACAACGTCGCCATCGAGACCATGGAAGACGAGAGCGGCTACATCATCGAGCCGTACCAGGCGCTGCGCG
AGCAGGTGGCCTGGGCGATTGCCAGCTTCTACCCCGAACTCGGCCTGCCCATTCCGGCGCTGAGCGAGAGCAGGGCGTCG
GGATTGTCGCTGCTGGGCCAGCGCCTGGTGTCGCGCGGCTACGTCAGTGAGGAGCAGGTCCTGTCGGCCCTGGAGCAGCA
GCGCAGCAGCGGCGTGTCGCTCGGTACGGTGCTGCTCGGCTCCAAGCTGCTGACCGAAGACCAGCTCTACGAGACGCTGG
CTGAGCAGGAAGACAGCCAGTATGTGCGCGACACCCGTGAATACCAGCCCACCGAGGAGGTGCTGGGGCTGCTGCTGCGT
GCCGATGCCCTGCGGCTGGCCTCGGTGCCGGTCAGTCAGGATGGAAACGTCGTCAGCGTCATTACCGGCGATTTGCGGCG
GCGTGAGGACATCGAGGCATTGATCGGCCACAAGGTCAGGCTGATGCTGGCCCGGCCCAGCGACGTGGATACCCTGATCG
ACCGCGCCTACCCGCAAAAGGGGAGACTCGGCGAGGCGATGGTGCAGCAGGGGACTCTGTCTCGGGCGCAACTGCGCGAG
GCGCTTCAGGTGCAGGCCCGCCAGGGCAAGGTCAAGCCGCTGGGCGAGGTCATCATGGACCTGGGCTTCGCGGGCAGCGA
GGAGATCGACGCGGCCCTGGTCAAACAGAACGCTGGGGGCGGCAGACTCGAAGACACCCTGGTGCAGTCGGGCAAGCTCA
GCCCCGAGATGCTGGCGCGCTCGCTGGCCGCCCAACTGGGGTATGAGTTCGTCGATCCGGTGACCTCGCCGCCCGACACC
AAGGTCGCCCTGATGATTCCCGAGGCCACCGCCCGGCGCTACACCGTGGTCCCGATGCGGCTGCAAGGCGAGTCACTGGT
CATTGCCATGAAAGACCCGCGCAACGTGTTCGCGCTCGACGACCTCAAGCTGATCACCGGGCGCGAGATCCTGCCCGCCG
TGATGGCCGAGAAGGACATCGTGCGCCTGATCGAGCGGTACTTCGGCGACAAGGACATGGCCAAGCTCAACGAGGAGCTG
GCCAAGTCCAGCCGTCAGAAGGACAAAGAAAAGGAGAGCGTCGACCTCTCGTCACTCGACGACAATGCGGTGGTGCGGGT
AATCGACGGCCTGATCCGTGAGGCCGCCCTGCAAGACGCCTCGGACATTCACATCGAACCCACCGAGCACAGCGTGCGGG
TGCGCTACCGCATCGACGGCTCGCTGCGCGAACACATGGAGCTGCCCAGGGGCGCGGCCCAGAGCATGCTGGCCCGCATC
AAGATCATGGGCAACCTTGACATCGCCGAGCGCCGCGTGCCGCAAGACGGCCGGGTGCGCTTCAAGAAGGGCAGCATCGA
CCTCGATCTGCGTCTCTCGACCCTGCCCACCGTCTACGGTGAGAAGGCCGTGATGCGCCTGCTGCAAAAAGCCAGCAACA
TTCCCGAAGTCGAGCAGCTCGGGTTTTCCGAGCACAATTTCCAGCGCTTTCTCGACGTGATCGAAAAGCCCAACGGCATC
TTTCTGGTGACCGGCCCCACCGGGTCGGGCAAGTCGTTTAGCAGCTTCTCGACTTTGAAGCGCATCGCCCGGCCCGACAA
GAACACCACCACCATCGAAGACCCCATCGAATATGAGATTCCCGGCATCATCCAATCACAGGTCAATACGGTGGCGGGCT
TTACCTTCGCCCGCGCCCTGAGGGCTTTTTTGCGCCAGGACCCTGACATCATCTTCGTGGGTGAGGTTCGCGACACCGAG
ACGGCCAAGATCGCCACCGAGGCGGCGCTGACCGGCCACCTGGTGCTGGCGACGCTTCACACCAACGACGCGCCGGGGGC
GATTACGCGTCTGGAAGAGATGGGCGTGGAGCCGTTTAACATCTCGGCCTCGGTGATCGGGGTGCTGGCGCAGCGGCTGG
TCCGCAAGGTCTGCACCGAGTGCAAGCGCCCCACCAACGCCGACCCGGAAGTGCTGCGGCGGCTCGGCATCGGCGAGCGC
GAACTCCGGGGCGCGAACCTGCAACGTGGCGCAGGCTGCCCGCGCTGCGGCGGCACCGGCTACAAGGGCCGCATGGGCAT
TCACGAACTGATGGTGGTGGACGACCCGATTCGCCGCGCCATCGGGACCCACCAGACCGCTGCCGAGATCCGTGAGGTCG
CCACCACCCAGAGCGGGATGAAGACCTTGCGCCAGGACGGCGTCGAGAAGGCCCTGATCGGCCTGACCACGCTCGAAGAA
GTGCTGGCCGTGACCAGCAACTGA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (201-301)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (43-145)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (367-471)

• Type II/IV secretion system protein (T2SSE) (512-776)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	71.991	100	0.722
pilF	Thermus thermophilus HB27	55.219	100	0.555