Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   N1249_RS18275 Genome accession   NZ_CP104014
Coordinates   3888052..3889059 (-) Length   335 a.a.
NCBI ID   WP_259831877.1    Uniprot ID   -
Organism   Enterobacter sp. CP102     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3883052..3894059
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N1249_RS18240 (N1249_18240) - 3883174..3883671 (+) 498 WP_014071691.1 SprT family zinc-dependent metalloprotease -
  N1249_RS18245 (N1249_18245) endA 3883766..3884473 (+) 708 WP_259831872.1 deoxyribonuclease I -
  N1249_RS18250 (N1249_18250) rsmE 3884526..3885257 (+) 732 WP_259831873.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  N1249_RS18255 (N1249_18255) gshB 3885277..3886224 (+) 948 WP_182062370.1 glutathione synthase -
  N1249_RS18260 (N1249_18260) - 3886299..3886859 (+) 561 WP_014071695.1 YqgE/AlgH family protein -
  N1249_RS18265 (N1249_18265) ruvX 3886859..3887275 (+) 417 WP_014071696.1 Holliday junction resolvase RuvX -
  N1249_RS18270 (N1249_18270) - 3887269..3887991 (-) 723 WP_259831875.1 IclR family transcriptional regulator -
  N1249_RS18275 (N1249_18275) pilT 3888052..3889059 (-) 1008 WP_259831877.1 type IV pilus twitching motility protein PilT Machinery gene
  N1249_RS18280 (N1249_18280) - 3889077..3889781 (+) 705 WP_217186910.1 YggS family pyridoxal phosphate-dependent enzyme -
  N1249_RS18285 (N1249_18285) - 3889800..3890366 (+) 567 WP_182062375.1 YggT family protein -
  N1249_RS18290 (N1249_18290) yggU 3890363..3890659 (+) 297 WP_014071701.1 DUF167 family protein YggU -
  N1249_RS18295 (N1249_18295) - 3890663..3891256 (+) 594 WP_259831880.1 XTP/dITP diphosphatase -
  N1249_RS18300 (N1249_18300) hemW 3891249..3892397 (+) 1149 WP_259831882.1 radical SAM family heme chaperone HemW -
  N1249_RS18305 (N1249_18305) - 3892454..3892792 (+) 339 WP_259831883.1 endonuclease domain-containing protein -
  N1249_RS18310 (N1249_18310) - 3892875..3893591 (-) 717 WP_259831885.1 DUF2884 domain-containing protein -
  N1249_RS18315 (N1249_18315) - 3893648..3893974 (-) 327 WP_182062380.1 YggL family protein -

Sequence

Protein


Download         Length: 335 a.a.        Molecular weight: 36624.59 Da        Isoelectric Point: 6.4251

>NTDB_id=727030 N1249_RS18275 WP_259831877.1 3888052..3889059(-) (pilT) [Enterobacter sp. CP102]
MVMEEIVALSVKHNVSDLHLCSDSPPRWRRAGTLEPAPFPPPDITQLLKTWLNDEQQGTWWANGQVDFAVTLASQQRLRG
SAFSHINGTSLTLRLLPASCPQLSALGAPRTLPELLDNDSGLILVTGATGSGKSTTLAAMVDHLNRHYDKHILTLEDPVE
VIYQSDRCLIHQREIGQHCPSFAEALRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLCRDVQGGRVALYELLVNTSAAANLIREGKTWQLPGIIQTGQQAGMQDFDQSFAER
RAQGRLHERSAVASG

Nucleotide


Download         Length: 1008 bp        

>NTDB_id=727030 N1249_RS18275 WP_259831877.1 3888052..3889059(-) (pilT) [Enterobacter sp. CP102]
GTGGTAATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAGTGATTCACCGCCACG
CTGGCGCAGGGCAGGTACGCTTGAACCTGCGCCATTCCCGCCGCCGGATATTACGCAGCTATTAAAAACGTGGCTTAACG
ATGAGCAACAGGGGACCTGGTGGGCAAACGGGCAGGTAGATTTTGCCGTGACGCTCGCCAGCCAGCAGCGTTTACGCGGC
AGCGCATTCAGCCACATTAACGGTACTTCACTCACGCTCAGACTCTTACCGGCGAGCTGCCCGCAGCTTTCGGCGCTGGG
GGCGCCGCGTACGCTCCCGGAACTGCTGGACAACGACAGCGGTCTGATTCTGGTGACGGGGGCAACAGGAAGTGGCAAAT
CCACTACGCTTGCGGCAATGGTGGATCATCTTAACCGCCACTACGATAAACACATTCTGACGCTGGAAGACCCGGTTGAG
GTTATTTATCAAAGCGATCGCTGTCTTATCCATCAGCGGGAGATAGGCCAGCACTGCCCTTCATTTGCAGAGGCACTTCG
CGCTGCATTACGTGAAGATCCTGACGTGATTTTATTGGGGGAGCTTCGCGACAGCGAAACGATCAGGCTCGCCCTTACGG
CGGCAGAAACTGGGCATCTGGTGCTGGCAACACTGCATACGCGTGGGGCGGCGCAGGCAATAGAGCGCCTGGTTGATACC
TTTCCGGCACAGGAAAAAGACCCGGTGCGTAATCAGCTGGCGGGCAGTTTGCGCGCGGTACTGGCGCAAAAGCTTTGTCG
GGACGTCCAGGGAGGGCGCGTGGCGCTGTACGAACTGCTGGTGAATACCTCAGCGGCAGCAAACCTTATCCGCGAGGGTA
AAACCTGGCAGTTGCCCGGCATTATCCAGACCGGGCAACAGGCGGGGATGCAGGATTTTGACCAAAGCTTTGCGGAACGC
AGAGCGCAGGGGCGGCTTCACGAAAGGTCAGCAGTTGCGTCGGGTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(63-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

50

97.91

0.49
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50

97.91

0.49
  pilT Pseudomonas stutzeri DSM 10701

46.407

99.701

0.463
  pilT Neisseria meningitidis 8013

46.646

97.91

0.457
  pilT Legionella pneumophila strain Lp02

47.205

96.119

0.454
  pilT Legionella pneumophila strain ERS1305867

47.205

96.119

0.454
  pilT Acinetobacter baumannii D1279779

45.509

99.701

0.454
  pilT Acinetobacter baumannii strain A118

45.509

99.701

0.454
  pilT Neisseria gonorrhoeae MS11

46.341

97.91

0.454
  pilT Acinetobacter nosocomialis M2

45.21

99.701

0.451
  pilT Pseudomonas aeruginosa PAK

45.21

99.701

0.451
  pilT Acinetobacter baylyi ADP1

46.177

97.612

0.451
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.188

95.522

0.403
  pilU Pseudomonas stutzeri DSM 10701

37.798

100

0.379
  pilU Vibrio cholerae strain A1552

38.154

97.015

0.37