Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   KU43P_RS01475 Genome accession   NZ_AP019365
Coordinates   308843..309841 (+) Length   332 a.a.
NCBI ID   WP_317660732.1    Uniprot ID   A0A5K7TPA9
Organism   Pseudomonas sp. KU43P     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 303843..314841
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KU43P_RS01445 (KU43P_02860) - 304319..304753 (-) 435 WP_317660727.1 DUF4426 domain-containing protein -
  KU43P_RS01450 (KU43P_02870) metW 304776..305396 (-) 621 WP_008092977.1 methionine biosynthesis protein MetW -
  KU43P_RS01455 (KU43P_02880) metX 305404..306543 (-) 1140 WP_317660728.1 homoserine O-succinyltransferase MetX -
  KU43P_RS01460 (KU43P_02890) - 306632..307222 (-) 591 WP_317660729.1 YggT family protein -
  KU43P_RS01465 (KU43P_02900) proC 307233..308051 (-) 819 WP_317660730.1 pyrroline-5-carboxylate reductase -
  KU43P_RS01470 (KU43P_02910) - 308091..308777 (-) 687 WP_317660731.1 YggS family pyridoxal phosphate-dependent enzyme -
  KU43P_RS01475 (KU43P_02920) pilT 308843..309841 (+) 999 WP_317660732.1 type IV pilus twitching motility protein PilT Machinery gene
  KU43P_RS01480 (KU43P_02930) - 309951..310580 (-) 630 WP_317660733.1 C40 family peptidase -
  KU43P_RS01485 (KU43P_02940) - 310750..311169 (+) 420 WP_317660734.1 NINE protein -
  KU43P_RS01490 (KU43P_02950) - 311219..311917 (-) 699 WP_317660735.1 SPOR domain-containing protein -
  KU43P_RS01495 (KU43P_02960) argS 311919..313655 (-) 1737 WP_317660736.1 arginine--tRNA ligase -

Sequence

Protein


Download         Length: 332 a.a.        Molecular weight: 36211.03 Da        Isoelectric Point: 6.8971

>NTDB_id=72418 KU43P_RS01475 WP_317660732.1 308843..309841(+) (pilT) [Pseudomonas sp. KU43P]
MDVTDLLARATEAGASDLHLAAGELPMLRLDGELQRLDLPCLRSAELYEGLAPLLEEGRRQCWERGDEQDLALELAPLGR
FRLNLFRQLNGPAATFRLIPRRIATLGELALEEVYRAIARHTDGLVLIGGPTGSGKSSTLAALIDQLNRERCLHIITLED
PVEVIHSGQRCLVNQREIGRHCRDFSHGLRSALRQDPDVIMIGELRDLETIRLALRAAETGHLVLATVHTRSAVSSIDRL
VDVFAAEEKPLVRAMLAESLRMVVAQVLVKRVGGGRVAAREVLVATPAVRNLIREGRMAQLCSVMQAGAGEGMRTMAGAM
SQLKIDGVIGLA

Nucleotide


Download         Length: 999 bp        

>NTDB_id=72418 KU43P_RS01475 WP_317660732.1 308843..309841(+) (pilT) [Pseudomonas sp. KU43P]
ATGGACGTGACCGACCTGCTGGCCCGGGCGACGGAGGCAGGGGCGAGCGACCTGCACCTGGCCGCGGGCGAATTACCGAT
GTTGCGCCTGGATGGCGAACTGCAACGGCTGGACCTGCCGTGTTTGCGGTCTGCTGAATTGTATGAGGGCTTGGCGCCTC
TGCTCGAGGAGGGGCGGCGCCAGTGCTGGGAAAGGGGCGATGAGCAGGATCTGGCCCTGGAGCTGGCGCCATTGGGACGC
TTTCGTCTCAATCTGTTTCGCCAGTTGAATGGCCCTGCGGCGACCTTTCGCCTCATTCCCAGACGTATCGCGACACTGGG
TGAGCTGGCGCTGGAAGAAGTGTATCGAGCTATTGCGCGGCACACCGATGGCCTGGTGCTGATCGGAGGGCCAACCGGCA
GCGGCAAGTCCAGCACCTTGGCGGCCCTGATCGATCAGCTCAATCGTGAGCGATGCCTGCACATCATTACCCTCGAAGAC
CCTGTCGAAGTTATCCACAGCGGGCAGCGCTGCTTGGTCAATCAACGCGAGATCGGCCGACACTGCCGGGATTTTTCCCA
CGGCTTGCGCAGTGCGCTGCGTCAGGACCCGGATGTGATCATGATCGGCGAGTTGCGAGATCTCGAGACGATACGCCTGG
CGTTGCGCGCGGCGGAAACGGGGCACCTGGTGCTGGCAACCGTGCATACGCGCTCGGCGGTGAGCAGCATCGACCGCTTG
GTGGATGTGTTTGCGGCAGAGGAAAAGCCATTGGTACGGGCCATGCTGGCGGAGTCGCTGCGCATGGTGGTGGCGCAGGT
GCTGGTCAAGCGTGTAGGGGGTGGGCGAGTGGCGGCCAGGGAAGTGCTGGTGGCGACGCCGGCAGTGCGCAACCTGATTC
GCGAAGGGCGCATGGCGCAGTTGTGTTCGGTGATGCAGGCGGGGGCTGGGGAGGGGATGCGGACGATGGCGGGGGCGATG
AGTCAGCTGAAGATCGATGGGGTGATCGGGCTTGCGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5K7TPA9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

51.662

99.699

0.515
  pilT Vibrio cholerae strain A1552

51.064

99.096

0.506
  pilT Pseudomonas aeruginosa PAK

51.064

99.096

0.506
  pilT Pseudomonas stutzeri DSM 10701

51.064

99.096

0.506
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.064

99.096

0.506
  pilT Acinetobacter baumannii strain A118

50.303

99.398

0.5
  pilT Acinetobacter nosocomialis M2

50.303

99.398

0.5
  pilT Acinetobacter baumannii D1279779

50.303

99.398

0.5
  pilT Legionella pneumophila strain Lp02

49.091

99.398

0.488
  pilT Legionella pneumophila strain ERS1305867

49.091

99.398

0.488
  pilT Neisseria meningitidis 8013

47.734

99.699

0.476
  pilT Neisseria gonorrhoeae MS11

47.432

99.699

0.473
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.679

97.59

0.446
  pilU Pseudomonas stutzeri DSM 10701

37.576

99.398

0.373
  pilU Vibrio cholerae strain A1552

37.855

95.482

0.361

Multiple sequence alignment