Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   M5T14_RS18365 Genome accession   NZ_CP103674
Coordinates   3861800..3862780 (-) Length   326 a.a.
NCBI ID   WP_259385060.1    Uniprot ID   -
Organism   Enterobacter hormaechei strain 1074     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3856800..3867780
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M5T14_RS18335 (M5T14_18335) - 3857678..3858175 (+) 498 WP_015571833.1 SprT family zinc-dependent metalloprotease -
  M5T14_RS18340 (M5T14_18340) endA 3858270..3858977 (+) 708 WP_045343971.1 deoxyribonuclease I -
  M5T14_RS18345 (M5T14_18345) rsmE 3859029..3859760 (+) 732 WP_017382923.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  M5T14_RS18350 (M5T14_18350) gshB 3859792..3860739 (+) 948 WP_015571835.1 glutathione synthase -
  M5T14_RS18355 (M5T14_18355) - 3860814..3861374 (+) 561 WP_006811924.1 YqgE/AlgH family protein -
  M5T14_RS18360 (M5T14_18360) ruvX 3861374..3861790 (+) 417 WP_006811925.1 Holliday junction resolvase RuvX -
  M5T14_RS18365 (M5T14_18365) pilT 3861800..3862780 (-) 981 WP_259385060.1 type IV pilus twitching motility protein PilT Machinery gene
  M5T14_RS18370 (M5T14_18370) - 3862798..3863499 (+) 702 WP_017692690.1 YggS family pyridoxal phosphate-dependent enzyme -
  M5T14_RS18375 (M5T14_18375) - 3863521..3864087 (+) 567 WP_015571838.1 YggT family protein -
  M5T14_RS18380 (M5T14_18380) yggU 3864084..3864380 (+) 297 WP_023304404.1 DUF167 family protein YggU -
  M5T14_RS18385 (M5T14_18385) - 3864384..3864977 (+) 594 WP_015571839.1 XTP/dITP diphosphatase -
  M5T14_RS18390 (M5T14_18390) hemW 3864970..3866118 (+) 1149 WP_017382926.1 radical SAM family heme chaperone HemW -
  M5T14_RS18395 (M5T14_18395) - 3866175..3866513 (+) 339 WP_023304405.1 endonuclease domain-containing protein -
  M5T14_RS18400 (M5T14_18400) - 3866597..3867313 (-) 717 WP_023304406.1 DUF2884 domain-containing protein -
  M5T14_RS18405 (M5T14_18405) - 3867371..3867697 (-) 327 WP_023304407.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35730.67 Da        Isoelectric Point: 6.5004

>NTDB_id=724041 M5T14_RS18365 WP_259385060.1 3861800..3862780(-) (pilT) [Enterobacter hormaechei strain 1074]
MDVEEIVAHSVKHNVSDLHLCSDSPPRWRRSGRLEPAPFPPPDVEALLKAWLNDEQQGAWWANGQVDFAVTLAGRQRLRG
SAFKHMHGVSIALRLLPLTCPQLSALGVPRAIPELLSNDNGLILVTGATGSGKSTTLAAMVDFLNHQTDGHILTLEDPVE
FMYQSERCLIQQREIGQHSPSFAEALRSALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLRQDVQGGRVALYELLVNTSAAANLIREGKTWQLPGIIQTGQQAGMQNFEQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=724041 M5T14_RS18365 WP_259385060.1 3861800..3862780(-) (pilT) [Enterobacter hormaechei strain 1074]
ATGGATGTGGAAGAAATTGTGGCCCATAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCGCG
CTGGCGCAGGTCAGGCCGTCTTGAACCTGCGCCGTTTCCGCCCCCGGATGTGGAGGCGTTATTAAAAGCGTGGCTCAACG
ACGAACAGCAGGGCGCATGGTGGGCAAACGGGCAGGTGGATTTTGCCGTTACCCTCGCAGGCCGTCAGCGGCTGCGCGGC
AGTGCGTTTAAGCATATGCACGGCGTTTCGATTGCGCTGCGGCTGTTGCCGCTGACGTGCCCGCAGCTCTCTGCGCTAGG
TGTGCCGCGAGCGATCCCGGAACTACTGTCCAATGACAATGGCCTGATTCTGGTCACCGGCGCCACCGGCAGTGGGAAAT
CGACCACCCTGGCCGCGATGGTCGATTTCCTCAATCACCAGACGGACGGGCATATTCTGACCCTGGAAGATCCGGTGGAG
TTTATGTACCAGAGCGAACGTTGCCTGATCCAGCAGCGGGAGATAGGCCAGCACAGCCCGTCCTTTGCCGAGGCGCTGCG
CAGCGCGCTGCGTGAAGATCCGGATGTGATTCTGCTTGGTGAGCTGCGCGACAGCGAGACGATACGCCTGGCGCTGACGG
CGGCGGAAACCGGCCATCTGGTGTTAGCCACGCTGCATACGCGCGGCGCGGCGCAGGCGATCGAGCGCCTGGTCGACACC
TTTCCGGCGCAGGAGAAAGATCCGGTGCGTAATCAGCTGGCGGGAAGCCTGCGTGCGGTCCTGGCGCAGAAGCTACGTCA
GGATGTCCAGGGCGGGCGCGTGGCGCTGTATGAGCTACTGGTGAATACCTCGGCGGCGGCAAACCTGATCCGCGAAGGCA
AAACCTGGCAACTGCCGGGCATTATTCAAACGGGGCAGCAGGCGGGAATGCAGAACTTTGAGCAGAGTCTGGCGGAGCGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(85-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.459

100

0.506
  pilT Vibrio cholerae strain A1552

50.459

100

0.506
  pilT Legionella pneumophila strain ERS1305867

48.758

98.773

0.482
  pilT Legionella pneumophila strain Lp02

48.758

98.773

0.482
  pilT Pseudomonas stutzeri DSM 10701

48.012

100

0.482
  pilT Neisseria meningitidis 8013

47.561

100

0.479
  pilT Neisseria gonorrhoeae MS11

47.256

100

0.475
  pilT Pseudomonas aeruginosa PAK

47.095

100

0.472
  pilT Acinetobacter baumannii D1279779

47.095

100

0.472
  pilT Acinetobacter baumannii strain A118

47.095

100

0.472
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Acinetobacter nosocomialis M2

47.5

98.16

0.466
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.034

99.08

0.426
  pilU Pseudomonas stutzeri DSM 10701

37.879

100

0.383
  pilU Vibrio cholerae strain A1552

37.888

98.773

0.374