Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   M5T15_RS18345 Genome accession   NZ_CP103494
Coordinates   3839974..3840954 (-) Length   326 a.a.
NCBI ID   WP_259429678.1    Uniprot ID   -
Organism   Enterobacter hormaechei strain 70     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3834974..3845954
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M5T15_RS18315 (M5T15_18315) - 3835852..3836349 (+) 498 WP_015571833.1 SprT family zinc-dependent metalloprotease -
  M5T15_RS18320 (M5T15_18320) endA 3836444..3837151 (+) 708 WP_003860034.1 deoxyribonuclease I -
  M5T15_RS18325 (M5T15_18325) rsmE 3837203..3837934 (+) 732 WP_017382923.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  M5T15_RS18330 (M5T15_18330) gshB 3837966..3838913 (+) 948 WP_259429677.1 glutathione synthase -
  M5T15_RS18335 (M5T15_18335) - 3838988..3839548 (+) 561 WP_006811924.1 YqgE/AlgH family protein -
  M5T15_RS18340 (M5T15_18340) ruvX 3839548..3839964 (+) 417 WP_006811925.1 Holliday junction resolvase RuvX -
  M5T15_RS18345 (M5T15_18345) pilT 3839974..3840954 (-) 981 WP_259429678.1 type IV pilus twitching motility protein PilT Machinery gene
  M5T15_RS18350 (M5T15_18350) - 3840972..3841673 (+) 702 WP_045141928.1 YggS family pyridoxal phosphate-dependent enzyme -
  M5T15_RS18355 (M5T15_18355) - 3841695..3842261 (+) 567 WP_015571838.1 YggT family protein -
  M5T15_RS18360 (M5T15_18360) yggU 3842258..3842554 (+) 297 WP_023304404.1 DUF167 family protein YggU -
  M5T15_RS18365 (M5T15_18365) - 3842558..3843151 (+) 594 WP_045141927.1 XTP/dITP diphosphatase -
  M5T15_RS18370 (M5T15_18370) hemW 3843144..3844292 (+) 1149 WP_045141926.1 radical SAM family heme chaperone HemW -
  M5T15_RS18375 (M5T15_18375) - 3844349..3844711 (+) 363 WP_023295414.1 endonuclease domain-containing protein -
  M5T15_RS18380 (M5T15_18380) - 3844871..3845587 (-) 717 WP_015571842.1 DUF2884 domain-containing protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35697.67 Da        Isoelectric Point: 6.3047

>NTDB_id=723043 M5T15_RS18345 WP_259429678.1 3839974..3840954(-) (pilT) [Enterobacter hormaechei strain 70]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRSGRLEPAPFPPPDVEALLKAWLNDEQQGAWWANGQVDFAVTLAGRQRLRG
SAFKHMLGVSIALRLLPLTCPQLSALGVPRAIPELLSNDNGLILVTGATGSGKSTTLAAMVDFLNHQTDGHILTLEDPVE
FMYQSERCLIQQREIGQHSPSFAEALRSALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDT
FPAQEKDPVRNQLAGSQRAVLAQKLRQDVQGGRVALYELLVNTSAAANLIREGKTWQLPGIIQTGQQAGMQNFEQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=723043 M5T15_RS18345 WP_259429678.1 3839974..3840954(-) (pilT) [Enterobacter hormaechei strain 70]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCGCG
CTGGCGCAGGTCAGGCCGTCTTGAACCTGCGCCGTTTCCGCCCCCGGATGTGGAGGCGTTATTAAAAGCGTGGCTCAACG
ACGAACAGCAGGGCGCATGGTGGGCAAACGGGCAGGTGGATTTTGCCGTTACCCTCGCAGGCCGTCAGCGGCTGCGCGGC
AGTGCGTTTAAGCATATGCTCGGCGTTTCGATTGCGCTGCGGCTGTTGCCGCTGACGTGCCCGCAGCTCTCTGCGCTAGG
TGTGCCGCGAGCGATCCCGGAACTACTGTCCAATGACAATGGCCTGATTCTGGTCACCGGCGCCACCGGCAGTGGGAAAT
CGACCACCCTGGCCGCTATGGTCGATTTCCTCAATCACCAGACGGACGGGCATATTCTGACCCTGGAAGATCCGGTGGAG
TTTATGTACCAGAGCGAACGTTGCCTGATCCAGCAGCGGGAGATAGGCCAGCACAGCCCGTCCTTTGCCGAGGCGCTGCG
CAGCGCGCTGCGTGAAGATCCGGATGTGATTCTGCTTGGTGAGCTGCGCGACAGCGAGACGATACGCCTGGCGCTGACGG
CGGCGGAAACCGGCCATCTGGTGTTAGCCACGCTGCACACGCGCGGCGCGGCGCAGGCGATCGAGCGCCTGGTCGACACC
TTTCCGGCGCAGGAGAAAGATCCGGTGCGTAATCAGCTGGCGGGAAGCCAGCGTGCGGTCCTGGCGCAGAAGCTACGTCA
GGATGTCCAGGGCGGGCGCGTGGCGCTGTATGAGCTACTGGTGAATACCTCGGCGGCGGCAAACCTGATCCGCGAAGGCA
AAACCTGGCAACTGCCGGGCATTATTCAAACGGGGCAGCAGGCGGGAATGCAGAACTTTGAGCAGAGTCTGGCGGAGCGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(108-244)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.153

100

0.503
  pilT Vibrio cholerae strain A1552

50.153

100

0.503
  pilT Legionella pneumophila strain Lp02

48.447

98.773

0.479
  pilT Legionella pneumophila strain ERS1305867

48.447

98.773

0.479
  pilT Pseudomonas stutzeri DSM 10701

47.706

100

0.479
  pilT Neisseria meningitidis 8013

47.256

100

0.475
  pilT Neisseria gonorrhoeae MS11

46.951

100

0.472
  pilT Acinetobacter baumannii strain A118

46.789

100

0.469
  pilT Pseudomonas aeruginosa PAK

46.789

100

0.469
  pilT Acinetobacter baumannii D1279779

46.789

100

0.469
  pilT Acinetobacter baylyi ADP1

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

47.188

98.16

0.463
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.034

99.08

0.426
  pilU Pseudomonas stutzeri DSM 10701

37.879

100

0.383
  pilU Vibrio cholerae strain A1552

37.578

98.773

0.371