Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NRD65_RS17690 Genome accession   NZ_CP103409
Coordinates   3724335..3725315 (-) Length   326 a.a.
NCBI ID   WP_110292353.1    Uniprot ID   -
Organism   Enterobacter hormaechei strain EHA64     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3719335..3730315
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NRD65_RS17660 (NRD65_17630) - 3720213..3720710 (+) 498 WP_110292351.1 SprT family zinc-dependent metalloprotease -
  NRD65_RS17665 (NRD65_17635) endA 3720805..3721512 (+) 708 WP_003860034.1 deoxyribonuclease I -
  NRD65_RS17670 (NRD65_17640) rsmE 3721564..3722295 (+) 732 WP_017382923.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NRD65_RS17675 (NRD65_17645) gshB 3722327..3723274 (+) 948 WP_015571835.1 glutathione synthase -
  NRD65_RS17680 (NRD65_17650) - 3723349..3723909 (+) 561 WP_006811924.1 YqgE/AlgH family protein -
  NRD65_RS17685 (NRD65_17655) ruvX 3723909..3724325 (+) 417 WP_006811925.1 Holliday junction resolvase RuvX -
  NRD65_RS17690 (NRD65_17660) pilT 3724335..3725315 (-) 981 WP_110292353.1 type IV pilus twitching motility protein PilT Machinery gene
  NRD65_RS17695 (NRD65_17665) - 3725333..3726034 (+) 702 WP_017692690.1 YggS family pyridoxal phosphate-dependent enzyme -
  NRD65_RS17700 (NRD65_17670) - 3726056..3726622 (+) 567 WP_015571838.1 YggT family protein -
  NRD65_RS17705 (NRD65_17675) yggU 3726619..3726915 (+) 297 WP_023304404.1 DUF167 family protein YggU -
  NRD65_RS17710 (NRD65_17680) rdgB 3726919..3727512 (+) 594 WP_110292354.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  NRD65_RS17715 (NRD65_17685) hemW 3727505..3728653 (+) 1149 WP_110292355.1 radical SAM family heme chaperone HemW -
  NRD65_RS17720 (NRD65_17690) - 3728710..3729048 (+) 339 WP_047726774.1 endonuclease domain-containing protein -
  NRD65_RS17725 (NRD65_17695) - 3729131..3729847 (-) 717 WP_015571842.1 DUF2884 domain-containing protein -
  NRD65_RS17730 (NRD65_17700) - 3729904..3730230 (-) 327 WP_003862421.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35766.78 Da        Isoelectric Point: 6.4131

>NTDB_id=722587 NRD65_RS17690 WP_110292353.1 3724335..3725315(-) (pilT) [Enterobacter hormaechei strain EHA64]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRSGRLEPAPFPPPDVEALLKAWLNDEQQGAWWANGQVDFAVTLAGRQRLRG
SAFKHMHGVSIALRLLPLTCPQLSALGVPRAIPELLFNDNGLILVTGATGSGKSTTLAAMVDFLNHQTDGHILTLEDPVE
FMYQSERCLIQQREIGQHSPSFAEALRSALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLRQDVQGGRVALYELLVNTSAAANLIREGKTWQLPGIIQTGQQAGMQNFEQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=722587 NRD65_RS17690 WP_110292353.1 3724335..3725315(-) (pilT) [Enterobacter hormaechei strain EHA64]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCGCG
CTGGCGCAGGTCAGGTCGTCTTGAACCTGCGCCGTTTCCGCCCCCGGATGTGGAGGCGTTATTAAAAGCGTGGCTCAACG
ACGAACAGCAGGGCGCATGGTGGGCAAACGGGCAGGTGGATTTTGCCGTTACCCTCGCAGGCCGTCAGCGGCTGCGCGGC
AGTGCGTTTAAGCATATGCACGGCGTTTCGATTGCGCTGCGGCTGTTGCCGCTGACGTGCCCGCAGCTCTCTGCGCTAGG
TGTGCCGCGAGCGATCCCGGAACTACTGTTCAATGACAATGGCCTGATTCTGGTCACCGGCGCCACCGGCAGTGGGAAAT
CGACCACCCTGGCCGCTATGGTCGATTTCCTCAATCACCAGACGGACGGGCATATTCTGACCCTGGAAGATCCGGTGGAG
TTTATGTACCAGAGCGAACGTTGCCTGATCCAGCAGCGGGAGATAGGCCAGCACAGCCCGTCCTTTGCCGAGGCGCTGCG
CAGCGCGCTGCGTGAAGATCCGGATGTGATTCTGCTTGGTGAGCTGCGCGACAGCGAGACGATACGCCTGGCGCTGACGG
CGGCGGAAACCGGCCATCTGGTGTTAGCCACGCTGCACACGCGCGGCGCGGCGCAGGCGATCGAGCGCCTGGTCGACACC
TTCCCGGCGCAGGAGAAAGATCCGGTGCGTAATCAGCTGGCGGGAAGCCTGCGTGCGGTCCTGGCGCAGAAGCTACGTCA
GGATGTCCAGGGCGGGCGCGTGGCGCTGTATGAGCTACTGGTGAATACCTCGGCGGCGGCAAACCTGATCCGCGAAGGCA
AAACCTGGCAACTGCCGGGCATTATTCAAACGGGGCAGCAGGCGGGAATGCAGAACTTTGAGCAGAGTCTGGCGGAGCGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(108-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.459

100

0.506
  pilT Vibrio cholerae strain A1552

50.459

100

0.506
  pilT Pseudomonas stutzeri DSM 10701

48.012

100

0.482
  pilT Neisseria meningitidis 8013

47.561

100

0.479
  pilT Legionella pneumophila strain Lp02

48.447

98.773

0.479
  pilT Legionella pneumophila strain ERS1305867

48.447

98.773

0.479
  pilT Neisseria gonorrhoeae MS11

47.256

100

0.475
  pilT Acinetobacter baumannii strain A118

47.095

100

0.472
  pilT Pseudomonas aeruginosa PAK

47.095

100

0.472
  pilT Acinetobacter baumannii D1279779

47.095

100

0.472
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Acinetobacter nosocomialis M2

47.5

98.16

0.466
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.344

99.08

0.429
  pilU Pseudomonas stutzeri DSM 10701

38.182

100

0.387
  pilU Vibrio cholerae strain A1552

37.888

98.773

0.374