Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NW905_RS24120 Genome accession   NZ_CP103054
Coordinates   5326439..5327443 (-) Length   334 a.a.
NCBI ID   WP_062575474.1    Uniprot ID   -
Organism   Pseudomonas mosselii strain P33     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5321439..5332443
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NW905_RS24100 (NW905_24100) argS 5322687..5324423 (+) 1737 WP_258800574.1 arginine--tRNA ligase -
  NW905_RS24105 (NW905_24105) - 5324425..5325129 (+) 705 WP_258800575.1 SPOR domain-containing protein -
  NW905_RS24110 (NW905_24110) - 5325169..5325594 (-) 426 WP_023630210.1 TM2 domain-containing protein -
  NW905_RS24115 (NW905_24115) - 5325752..5326399 (+) 648 WP_062575473.1 C40 family peptidase -
  NW905_RS24120 (NW905_24120) pilT 5326439..5327443 (-) 1005 WP_062575474.1 type IV pilus twitching motility protein PilT Machinery gene
  NW905_RS24125 (NW905_24125) - 5327509..5328189 (+) 681 WP_258800576.1 YggS family pyridoxal phosphate-dependent enzyme -
  NW905_RS24130 (NW905_24130) proC 5328234..5329052 (+) 819 WP_062575476.1 pyrroline-5-carboxylate reductase -
  NW905_RS24135 (NW905_24135) - 5329063..5329653 (+) 591 WP_028689124.1 YggT family protein -
  NW905_RS24140 (NW905_24140) - 5329742..5330881 (+) 1140 WP_062575477.1 homoserine O-acetyltransferase -
  NW905_RS24145 (NW905_24145) metW 5330889..5331509 (+) 621 WP_011531735.1 methionine biosynthesis protein MetW -
  NW905_RS24150 (NW905_24150) - 5331535..5331969 (+) 435 WP_096049167.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 334 a.a.        Molecular weight: 36227.83 Da        Isoelectric Point: 6.9184

>NTDB_id=721204 NW905_RS24120 WP_062575474.1 5326439..5327443(-) (pilT) [Pseudomonas mosselii strain P33]
MDVTDLLRRALAAAASDLHLAAGERPLLRVDGVLQRLDLPVLTAGALEDGMAAVLDDEQRRQWAAGEELDLAVSLDGLGR
FRLNLFRQRNGPSASVRLIPASVPCLEVLELVDVFQIISRYRDGLILVGGATGSGKSSTLAALIDRLNREYALHIVTLED
PIEFIHASQRSLVNQREVGLHSRDFAQALRSALRQDPDVIMIGELRDLHSIRLAMRAAETGHLVLATVHTRSAVNSIDRL
VEVFAPEEKPLVRSMLADSLRLVVAQVLVRRSGGGRVAAREVLVATPAVRNLIREGRLAQIHSLMQAGAAVGMCTMEGAL
QALEARGIIDRASN

Nucleotide


Download         Length: 1005 bp        

>NTDB_id=721204 NW905_RS24120 WP_062575474.1 5326439..5327443(-) (pilT) [Pseudomonas mosselii strain P33]
ATGGATGTGACCGACCTGTTGCGCAGGGCCCTGGCTGCCGCTGCCAGCGACCTGCACCTGGCCGCGGGCGAGCGGCCGCT
GCTGCGCGTGGATGGGGTGTTGCAGCGCCTGGACCTGCCGGTGCTGACGGCAGGAGCGCTGGAAGACGGGATGGCTGCCG
TGCTTGATGACGAGCAGCGGCGACAGTGGGCTGCCGGCGAAGAGTTGGACCTGGCGGTGAGTCTGGATGGGCTGGGGCGC
TTTCGGCTGAACCTGTTCCGCCAGCGCAATGGCCCGAGTGCCAGCGTGCGTTTGATCCCCGCGAGCGTGCCCTGTCTGGA
GGTGCTTGAACTGGTAGATGTATTTCAAATCATCTCCCGGTACCGGGATGGCTTGATCCTGGTGGGTGGGGCCACCGGCA
GCGGCAAGTCGAGCACCCTGGCGGCGCTGATCGATCGGCTCAACCGCGAATACGCATTGCATATCGTCACGCTCGAGGAC
CCGATCGAGTTCATCCACGCCAGCCAGCGCAGCCTGGTCAACCAGCGTGAGGTCGGCCTGCATAGCCGTGACTTTGCCCA
GGCCCTGCGCAGCGCCCTGCGTCAGGACCCGGACGTGATCATGATCGGTGAGCTGCGCGATCTGCACAGCATTCGCCTGG
CGATGCGCGCCGCGGAGACCGGGCACCTGGTGCTGGCCACCGTGCATACGCGATCGGCGGTGAACAGTATCGACCGGCTG
GTGGAGGTGTTCGCGCCGGAGGAAAAGCCGCTGGTGCGCAGCATGCTGGCCGACTCCCTGCGGCTGGTGGTTGCGCAGGT
GCTGGTTCGGCGTTCAGGCGGCGGTCGGGTCGCGGCGCGGGAGGTGCTGGTGGCGACGCCGGCGGTGCGCAACCTGATCC
GCGAGGGGCGTCTGGCGCAGATTCATTCGCTGATGCAGGCTGGTGCGGCTGTGGGGATGTGCACGATGGAGGGGGCGCTG
CAGGCGCTGGAAGCGCGGGGGATTATCGACAGGGCCTCAAACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

49.55

99.701

0.494
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.697

98.802

0.491
  pilT Vibrio cholerae strain A1552

49.697

98.802

0.491
  pilT Pseudomonas stutzeri DSM 10701

49.249

99.701

0.491
  pilT Acinetobacter baylyi ADP1

49.394

98.802

0.488
  pilT Neisseria meningitidis 8013

48.788

98.802

0.482
  pilT Acinetobacter baumannii strain A118

48.936

98.503

0.482
  pilT Acinetobacter baumannii D1279779

48.936

98.503

0.482
  pilT Neisseria gonorrhoeae MS11

48.485

98.802

0.479
  pilT Acinetobacter nosocomialis M2

48.632

98.503

0.479
  pilT Legionella pneumophila strain Lp02

48.036

99.102

0.476
  pilT Legionella pneumophila strain ERS1305867

48.036

99.102

0.476
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.061

98.802

0.455
  pilU Pseudomonas stutzeri DSM 10701

38.788

98.802

0.383
  pilU Vibrio cholerae strain A1552

39.198

97.006

0.38