Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   EoCCA6_RS07770 Genome accession   NZ_AP019007
Coordinates   1642712..1643692 (-) Length   326 a.a.
NCBI ID   WP_152082190.1    Uniprot ID   -
Organism   Enterobacter oligotrophicus strain CCA6     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1637712..1648692
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EoCCA6_RS07740 - 1638600..1639097 (+) 498 WP_003860035.1 SprT family zinc-dependent metalloprotease -
  EoCCA6_RS07745 endA 1639192..1639899 (+) 708 WP_152082185.1 deoxyribonuclease I -
  EoCCA6_RS07750 rsmE 1639952..1640683 (+) 732 WP_152082186.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  EoCCA6_RS07755 gshB 1640703..1641650 (+) 948 WP_152082187.1 glutathione synthase -
  EoCCA6_RS07760 - 1641725..1642285 (+) 561 WP_152082188.1 YqgE/AlgH family protein -
  EoCCA6_RS07765 ruvX 1642285..1642701 (+) 417 WP_152082189.1 Holliday junction resolvase RuvX -
  EoCCA6_RS07770 pilT 1642712..1643692 (-) 981 WP_152082190.1 type IV pilus twitching motility protein PilT Machinery gene
  EoCCA6_RS07775 - 1643710..1644414 (+) 705 WP_152082191.1 YggS family pyridoxal phosphate-dependent enzyme -
  EoCCA6_RS07780 - 1644433..1644999 (+) 567 WP_152082192.1 YggT family protein -
  EoCCA6_RS07785 yggU 1644996..1645292 (+) 297 WP_152082193.1 DUF167 family protein YggU -
  EoCCA6_RS07790 - 1645296..1645889 (+) 594 WP_152082194.1 XTP/dITP diphosphatase -
  EoCCA6_RS07795 hemW 1645882..1647024 (+) 1143 WP_152082195.1 radical SAM family heme chaperone HemW -
  EoCCA6_RS07800 - 1647088..1647450 (+) 363 WP_152082196.1 endonuclease domain-containing protein -
  EoCCA6_RS07805 - 1647512..1648231 (-) 720 WP_152082197.1 DUF2884 domain-containing protein -
  EoCCA6_RS07810 - 1648285..1648611 (-) 327 WP_152082198.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35728.74 Da        Isoelectric Point: 6.2330

>NTDB_id=72038 EoCCA6_RS07770 WP_152082190.1 1642712..1643692(-) (pilT) [Enterobacter oligotrophicus strain CCA6]
MDMEEIVALSVKHNVSDLHLCSDSPPRWRRLGRLEPAPFPSPDVEALLKTWLSDEQQGAWWANGQVDFAVTLAGNQRLRG
SAFNHMKGISVVLRLLPLSCPQLSTLGVPRAIPELLSNDSGLILVTGATGSGKSTTLAAMVDFLNHHTDGHILTLEDPVE
FMYQSERCLIQQREIGQHAPSFAEALRSALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAPQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLRQDIQGGRVALYELLVNTSAAANLIREGKTWQLPGIIQTGQQAGMQNFDQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=72038 EoCCA6_RS07770 WP_152082190.1 1642712..1643692(-) (pilT) [Enterobacter oligotrophicus strain CCA6]
ATGGATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCCCCCCCGCG
CTGGCGCAGATTAGGGCGTCTTGAGCCTGCGCCGTTTCCCTCGCCTGACGTGGAAGCGCTATTAAAAACGTGGCTCAGCG
ATGAGCAGCAGGGGGCGTGGTGGGCAAACGGGCAGGTAGATTTTGCCGTCACGCTCGCAGGCAATCAGCGCCTGCGCGGT
AGTGCGTTTAACCACATGAAGGGGATTTCAGTCGTGCTTCGGCTGTTGCCGCTTTCCTGCCCGCAACTCTCCACGCTGGG
TGTGCCGCGGGCGATCCCGGAGCTGCTATCCAATGACAGCGGACTGATTTTGGTCACAGGGGCGACCGGAAGCGGTAAAT
CCACCACGCTCGCGGCGATGGTCGATTTTCTCAACCATCATACTGACGGGCATATTCTGACGCTCGAAGATCCGGTGGAA
TTTATGTACCAGAGTGAACGTTGCCTGATTCAGCAACGGGAGATAGGTCAGCACGCTCCGTCATTTGCCGAGGCGCTACG
CAGTGCGCTACGTGAAGATCCGGACGTCATTTTACTGGGCGAACTGCGCGACAGCGAAACCATCCGCCTGGCGCTCACGG
CGGCGGAAACTGGCCATCTGGTGCTGGCGACGCTGCATACACGCGGCGCTCCGCAGGCTATCGAACGGCTGGTGGATACC
TTCCCGGCGCAGGAGAAAGATCCGGTGCGTAACCAGCTGGCGGGTAGCCTGCGTGCGGTACTGGCGCAAAAGCTGCGTCA
GGATATTCAGGGTGGGCGCGTTGCGCTTTATGAGTTGCTGGTGAACACATCGGCGGCGGCAAATCTCATCCGCGAAGGAA
AAACGTGGCAATTGCCCGGCATCATACAAACCGGTCAGCAGGCAGGGATGCAGAATTTCGACCAGAGCCTGGCGGAAAGA
AGGGCGCAGGGGCGACTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(74-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.847

100

0.5
  pilT Vibrio cholerae strain A1552

49.847

100

0.5
  pilT Legionella pneumophila strain ERS1305867

49.068

98.773

0.485
  pilT Legionella pneumophila strain Lp02

49.068

98.773

0.485
  pilT Pseudomonas stutzeri DSM 10701

48.012

100

0.482
  pilT Neisseria meningitidis 8013

47.561

100

0.479
  pilT Pseudomonas aeruginosa PAK

47.706

100

0.479
  pilT Acinetobacter baumannii strain A118

47.401

100

0.475
  pilT Acinetobacter baylyi ADP1

47.401

100

0.475
  pilT Acinetobacter baumannii D1279779

47.401

100

0.475
  pilT Neisseria gonorrhoeae MS11

47.256

100

0.475
  pilT Acinetobacter nosocomialis M2

47.812

98.16

0.469
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.724

99.08

0.423
  pilU Pseudomonas stutzeri DSM 10701

38.182

100

0.387
  pilU Vibrio cholerae strain A1552

38.509

98.773

0.38

Multiple sequence alignment