Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   NVV94_RS01965 Genome accession   NZ_CP102951
Coordinates   404714..406768 (+) Length   684 a.a.
NCBI ID   WP_258445587.1    Uniprot ID   -
Organism   Pseudomonas sp. LS1212     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 399714..411768
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NVV94_RS01945 (NVV94_01945) pilM 401908..402972 (+) 1065 WP_258445583.1 pilus assembly protein PilM Machinery gene
  NVV94_RS01950 (NVV94_01950) - 402972..403538 (+) 567 WP_258445584.1 PilN domain-containing protein -
  NVV94_RS01955 (NVV94_01955) - 403535..404158 (+) 624 WP_258445585.1 type 4a pilus biogenesis protein PilO -
  NVV94_RS01960 (NVV94_01960) - 404155..404682 (+) 528 WP_258445586.1 pilus assembly protein PilP -
  NVV94_RS01965 (NVV94_01965) pilQ 404714..406768 (+) 2055 WP_258445587.1 type IV pilus secretin PilQ Machinery gene
  NVV94_RS01970 (NVV94_01970) aroK 406773..407291 (+) 519 WP_258445588.1 shikimate kinase AroK -
  NVV94_RS01975 (NVV94_01975) aroB 407429..408526 (+) 1098 WP_258445589.1 3-dehydroquinate synthase -
  NVV94_RS01980 (NVV94_01980) - 408543..410159 (+) 1617 WP_258445590.1 AAA family ATPase -

Sequence

Protein


Download         Length: 684 a.a.        Molecular weight: 73929.18 Da        Isoelectric Point: 5.5659

>NTDB_id=720167 NVV94_RS01965 WP_258445587.1 404714..406768(+) (pilQ) [Pseudomonas sp. LS1212]
MNRILSAVGIALWIALMSPTVLATTLKTLEVAALPGDRVELKLSFDGPAPAPKGYATEQPARIALDLPGVTNGLAVKRRD
LGSGNARSVVVVEAKERTRLVVNLAAQAPYSTRVEGNDLVLVIGQGSDALPGRAPGQTQVSPTPAPARVPAASGRAIRNI
DFQRGELGEGNVVIELSDPTISADIKEQGGKIRLNFARTRLPEPLRVRLDVKNFATPVQFVSATADGGTSSVSIEPGGAF
DYSAYQTDNKLTISVRPLHSEDLQQRKADRFAYSGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTIQGGITLRLQNVP
WDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADIAKLFQSVTNAQAQE
QERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGATNLKGSSNWTAHGLG
GDSIGPNVPFVDMGVANATSGIGLGFVTDNVLLDLELSAMEKTGNGEIVSQPKVVTSDKETAKILKGTEVPYQEASSSGA
TSVSFKEASLSLEVTPQITPDSRIIMEVKVTKDEPDYLNMVQNVPPIKKNEVNAKVLVNDGETIVIGGVFSNTQSKTVDK
VPFLGDVPYVGRLFRRDAVQEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2055 bp        

>NTDB_id=720167 NVV94_RS01965 WP_258445587.1 404714..406768(+) (pilQ) [Pseudomonas sp. LS1212]
ATGAACAGGATTCTATCTGCCGTTGGCATTGCGCTATGGATAGCGCTGATGTCGCCCACGGTGTTGGCCACTACCCTCAA
GACCCTGGAGGTAGCAGCGCTGCCCGGTGATCGGGTGGAGCTGAAGCTTTCGTTCGACGGCCCGGCCCCTGCGCCAAAGG
GCTATGCCACCGAGCAGCCGGCGCGAATCGCGCTGGACTTGCCCGGTGTAACCAATGGGCTGGCGGTGAAGCGCCGCGAC
CTGGGCTCGGGCAATGCCCGCAGCGTCGTTGTCGTGGAGGCGAAGGAGCGGACACGCCTGGTCGTCAACCTGGCGGCGCA
GGCGCCCTACAGCACGCGGGTCGAAGGCAATGATCTGGTACTGGTGATCGGCCAGGGTAGCGACGCGTTGCCAGGCCGCG
CGCCAGGACAGACGCAAGTGTCGCCGACACCCGCCCCGGCGCGGGTTCCAGCAGCTTCCGGCAGGGCGATCAGGAACATC
GACTTTCAACGCGGCGAGTTGGGCGAAGGCAATGTGGTCATCGAACTGAGCGACCCGACCATCAGCGCCGATATCAAGGA
ACAAGGCGGCAAGATTCGTCTGAATTTCGCCAGGACCCGCTTGCCCGAGCCGCTGCGCGTGCGACTGGACGTGAAAAATT
TCGCCACGCCTGTTCAGTTCGTCAGCGCCACAGCCGATGGCGGTACCAGCAGTGTCAGCATCGAGCCAGGCGGGGCCTTC
GATTATTCGGCCTATCAGACAGACAACAAACTGACCATCAGCGTCAGGCCGCTGCACAGCGAAGACCTGCAGCAGCGCAA
GGCTGACCGCTTCGCATACAGCGGGGAGAAGCTATCGCTGAACTTCCAGGATATCGACGTGCGCTCGGTGTTGCAGTTGA
TCGCCGACTTCACCAACCTCAACCTGGTCGCCAGTGACACGATTCAGGGCGGTATCACCTTGCGCCTGCAGAACGTGCCG
TGGGACCAGGCGCTGGACCTGGTGCTCAAGACCAAGGGCCTGGACAAGCGCAAGGTCGGCAATGTGTTGCTGGTGGCCCC
GGCCGACGAAATCGCTGCCCGCGAGCGGCAGGAGCTGGAGTCGCAGAAGCAGATCGCCGAGCTGGCGCCCCTGCGCCGGG
AGCTGTTGCAGGTCAACTATGCCAAGGCGGCAGACATCGCCAAGCTGTTCCAGTCGGTGACCAATGCCCAGGCACAGGAG
CAGGAGCGCGGCTCGATCACGGTCGACGAGAGAACCAACAATATCATCGCCTACCAGACCCAGGATCGCCTGGACGAGCT
ACGCCGGATCGTTGCGCAACTGGATATTCCGGTCCGTCAGGTCATGATCGAGGCGCGCATCGTCGAGGCCAACGTCGACT
ATGACAAGAGCCTTGGCGTGCGCTGGGGAGGTGCGACCAACCTGAAGGGCAGCAGCAACTGGACCGCCCATGGGCTCGGT
GGCGACAGCATCGGCCCCAACGTGCCCTTCGTGGACATGGGGGTCGCCAATGCGACATCAGGTATCGGCCTGGGCTTCGT
CACCGATAATGTGTTGCTGGACCTGGAGCTCAGTGCGATGGAGAAAACCGGTAACGGTGAAATCGTCTCTCAGCCCAAAG
TGGTCACTTCTGACAAGGAAACCGCGAAAATCCTCAAGGGCACCGAAGTGCCCTATCAAGAGGCGAGCTCCAGCGGCGCG
ACCTCGGTCTCGTTCAAGGAAGCTTCGTTATCGCTGGAAGTCACCCCGCAGATTACCCCCGACAGTCGCATCATCATGGA
GGTAAAGGTGACCAAGGATGAGCCGGATTACCTCAACATGGTGCAGAATGTGCCGCCGATCAAGAAAAACGAGGTCAACG
CCAAGGTGTTGGTCAATGATGGCGAAACCATCGTCATCGGCGGTGTTTTCTCGAATACGCAAAGTAAAACCGTAGATAAG
GTGCCATTTCTTGGCGATGTACCGTATGTTGGCCGCCTCTTTCGCCGCGATGCCGTGCAAGAGAAAAAATCCGAGCTGCT
GGTATTCCTGACACCGCGTATCATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(301-347)

Bacterial type II/III secretion system short domain (Secretin_N)

(375-440)

Bacterial type II and III secretion system protein (Secretin)

(519-675)

AMIN domain (AMIN)

(37-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

71.13

100

0.746
  comQ Acinetobacter baylyi ADP1

43.043

100

0.434
  pilQ Acinetobacter baumannii D1279779

40.027

100

0.431
  pilQ Acinetobacter baumannii strain A118

40.027

100

0.431
  pilQ Legionella pneumophila strain ERS1305867

38.633

98.392

0.38
  pilQ Legionella pneumophila strain Lp02

38.45

98.099

0.377