Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   NUG21_RS05720 Genome accession   NZ_CP102594
Coordinates   1332555..1332974 (+) Length   139 a.a.
NCBI ID   WP_019799634.1    Uniprot ID   -
Organism   Xanthomonas sp. CFBP 8445     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1327555..1337974
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NUG21_RS05700 (NUG21_05700) - 1327974..1329308 (+) 1335 WP_160947577.1 HAMP domain-containing sensor histidine kinase -
  NUG21_RS05705 (NUG21_05705) coaE 1329418..1330047 (-) 630 WP_019797203.1 dephospho-CoA kinase -
  NUG21_RS05710 (NUG21_05710) - 1330061..1330924 (-) 864 WP_010342233.1 A24 family peptidase -
  NUG21_RS05715 (NUG21_05715) pilC 1330931..1332190 (-) 1260 WP_263393035.1 type II secretion system F family protein Machinery gene
  NUG21_RS05720 (NUG21_05720) pilA/pilAI 1332555..1332974 (+) 420 WP_019799634.1 pilin Machinery gene
  NUG21_RS05725 (NUG21_05725) - 1333008..1333481 (+) 474 WP_160947579.1 pilin -
  NUG21_RS05730 (NUG21_05730) pilB 1333522..1335255 (+) 1734 WP_160947580.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NUG21_RS05735 (NUG21_05735) pilR 1335417..1336805 (-) 1389 WP_242080579.1 sigma-54 dependent transcriptional regulator Regulator

Sequence

Protein


Download         Length: 139 a.a.        Molecular weight: 14431.57 Da        Isoelectric Point: 8.4733

>NTDB_id=717986 NUG21_RS05720 WP_019799634.1 1332555..1332974(+) (pilA/pilAI) [Xanthomonas sp. CFBP 8445]
MKKQQGFTLIELMIVVAIIAILAAIAIPMYQDYVVKSQATSALAEITPGKVGFETAINQGKTPSTTATDDGYIGIPATTT
YCNVTLGATATGNITCTAKGGNAGKFNTKTIIWTRDANGAWTCTSTLDAKYRPGSCAAG

Nucleotide


Download         Length: 420 bp        

>NTDB_id=717986 NUG21_RS05720 WP_019799634.1 1332555..1332974(+) (pilA/pilAI) [Xanthomonas sp. CFBP 8445]
ATGAAGAAGCAACAGGGCTTTACCCTGATCGAACTGATGATCGTGGTTGCGATCATCGCCATCCTGGCCGCTATCGCGAT
CCCGATGTACCAGGACTATGTCGTCAAGTCCCAGGCGACCTCGGCTCTGGCTGAAATCACGCCTGGGAAGGTGGGTTTTG
AGACCGCGATTAACCAGGGCAAGACTCCCAGCACCACTGCGACGGATGATGGCTACATCGGCATTCCGGCTACCACCACC
TATTGCAACGTTACGCTGGGTGCCACTGCTACTGGCAATATCACGTGTACCGCGAAGGGCGGAAATGCCGGTAAGTTCAA
CACCAAGACGATCATCTGGACGCGCGACGCAAACGGTGCATGGACTTGCACCAGCACCCTGGACGCCAAGTATCGTCCGG
GCAGTTGCGCTGCGGGCTGA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-136)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

70.149

96.403

0.676
  pilA/pilAII Pseudomonas stutzeri DSM 10701

61.364

94.964

0.583
  pilA Pseudomonas aeruginosa PAK

45.638

100

0.489
  pilA Acinetobacter baumannii strain A118

47.222

100

0.489
  pilA2 Legionella pneumophila strain ERS1305867

47.826

99.281

0.475
  pilA2 Legionella pneumophila str. Paris

47.826

99.281

0.475
  pilA Vibrio cholerae strain A1552

44.755

100

0.46
  pilA Vibrio cholerae C6706

44.755

100

0.46
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

44.755

100

0.46
  comP Acinetobacter baylyi ADP1

41.558

100

0.46
  pilA Ralstonia pseudosolanacearum GMI1000

36.747

100

0.439
  pilA Vibrio parahaemolyticus RIMD 2210633

39.535

92.806

0.367