Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NUG21_RS05730 Genome accession   NZ_CP102594
Coordinates   1333522..1335255 (+) Length   577 a.a.
NCBI ID   WP_160947580.1    Uniprot ID   -
Organism   Xanthomonas sp. CFBP 8445     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1328522..1340255
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NUG21_RS05705 (NUG21_05705) coaE 1329418..1330047 (-) 630 WP_019797203.1 dephospho-CoA kinase -
  NUG21_RS05710 (NUG21_05710) - 1330061..1330924 (-) 864 WP_010342233.1 A24 family peptidase -
  NUG21_RS05715 (NUG21_05715) pilC 1330931..1332190 (-) 1260 WP_263393035.1 type II secretion system F family protein Machinery gene
  NUG21_RS05720 (NUG21_05720) pilA/pilAI 1332555..1332974 (+) 420 WP_019799634.1 pilin Machinery gene
  NUG21_RS05725 (NUG21_05725) - 1333008..1333481 (+) 474 WP_160947579.1 pilin -
  NUG21_RS05730 (NUG21_05730) pilB 1333522..1335255 (+) 1734 WP_160947580.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NUG21_RS05735 (NUG21_05735) pilR 1335417..1336805 (-) 1389 WP_242080579.1 sigma-54 dependent transcriptional regulator Regulator
  NUG21_RS05740 (NUG21_05740) - 1336888..1338501 (-) 1614 WP_263392515.1 ATP-binding protein -
  NUG21_RS05745 (NUG21_05745) sucC 1338879..1340048 (+) 1170 WP_010342227.1 ADP-forming succinate--CoA ligase subunit beta -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62662.96 Da        Isoelectric Point: 5.8239

>NTDB_id=717987 NUG21_RS05730 WP_160947580.1 1333522..1335255(+) (pilB) [Xanthomonas sp. CFBP 8445]
MSALMTPNLVGITGIARRLVQDGALDEAAARAAMAQAGEAKVPLPQWFSEKKLVTAAQLAAANAVEFGMPLMDVSVFDAS
QNAMKLVSEELLQKHQVLPLFKRGSRLFVGISNPTQTRALDDIKFHTNLTVEPILVDEDQIRRTLEQWQASNDSLGSALG
DDEGMDNLDVGGGDEDMGSSGDTGIDAKGEDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKSVAKAPV
KLNQRIAARLKVMAQLDIAEKRVPQDGRIKLNLSKNKQIDFRVSTLPTLFGEKIVLRILDGSAAKLGIDKLGYEPEQQKL
FLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDDTRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPD
IIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRPVQLPE
KALLAEGFTEAEVAAGIQLYEAVGCDDCTEGYKGRTGIYQVMPMNDEIAAIVLQGGNAMDIASAAQKIGVKDLRQSALLK
ARNGITSLAEINRVTKD

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=717987 NUG21_RS05730 WP_160947580.1 1333522..1335255(+) (pilB) [Xanthomonas sp. CFBP 8445]
ATGAGCGCGTTGATGACTCCAAATCTGGTCGGCATCACCGGTATCGCCCGCCGTCTCGTGCAGGATGGCGCGCTGGACGA
AGCTGCGGCGCGGGCCGCGATGGCCCAGGCGGGCGAGGCCAAGGTGCCGCTGCCGCAGTGGTTCTCCGAGAAGAAGCTGG
TCACTGCCGCGCAACTGGCCGCCGCCAACGCGGTGGAGTTCGGCATGCCGTTGATGGATGTCTCGGTTTTCGACGCCAGC
CAGAACGCGATGAAGCTGGTCAGCGAGGAACTGCTGCAGAAGCATCAGGTGCTGCCGTTGTTCAAGCGCGGCAGCCGCTT
GTTCGTCGGCATCAGCAATCCGACCCAGACCCGGGCGCTGGATGACATCAAGTTCCACACCAACCTGACGGTAGAGCCGA
TCCTGGTCGACGAGGACCAGATCCGCCGGACCCTGGAGCAGTGGCAGGCCAGCAACGACTCGCTCGGCAGCGCGCTGGGC
GACGACGAGGGCATGGACAACCTCGATGTCGGCGGCGGCGACGAGGACATGGGCAGCAGCGGCGACACCGGCATCGACGC
CAAGGGCGAGGACACCCCGGTCGTCAAGTTCGTGAACAAGGTGCTGGTCGATGCGATCCGCCGCGGCGCGTCCGACATCC
ATTTCGAGCCCTACGAGGACGACTACCGGGTGCGCCTGCGCATCGACGGCCTCCTCAAGAGCGTGGCCAAGGCGCCGGTC
AAGCTCAACCAGCGCATCGCCGCGCGCCTGAAGGTGATGGCGCAGCTGGACATCGCCGAGAAGCGGGTCCCGCAGGACGG
TCGCATCAAGCTCAACTTGTCCAAGAACAAGCAGATCGACTTCCGTGTGAGCACGCTGCCGACGTTGTTCGGCGAGAAGA
TCGTGCTGCGTATCCTCGACGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTCGGTTACGAGCCGGAGCAGCAGAAGCTG
TTCCTGGATGCGATCCACAAACCGTATGGCATGGTCCTGGTCACCGGTCCCACCGGCTCGGGCAAGACGGTGTCGCTGTA
CACCGCGCTGGGCATCCTCAACGACGACACCCGCAACATCTCCACCGCCGAGGATCCGGTCGAAATCCGCCTGCCCGGGG
TCAACCAGGTGCAGCAGAACAACAAGCGCGGCATGACCTTCGCCGCGGCGCTGCGCTCGTTCCTGCGCCAGGATCCGGAC
ATCATCATGGTCGGCGAAATCCGCGATCTGGAGACCGCCGAGATCGCGATCAAGGCTGCGCAGACCGGTCACATGGTGCT
GTCCACCCTGCACACCAACGATGCGCCGCAGACCATCGCGCGCCTGATGAACATGGGCATCGCGCCCTACAACATCACCA
GTTCGGTAACCCTGGTCATCGCCCAGCGCCTGGCGCGGCGCCTGTGCAACAACTGCAAGCGCCCGGTGCAGTTGCCGGAG
AAGGCCCTGCTGGCCGAGGGCTTCACCGAGGCGGAGGTCGCCGCCGGCATCCAGTTGTACGAGGCGGTCGGCTGCGACGA
CTGTACCGAGGGCTACAAGGGCCGCACCGGCATCTACCAGGTGATGCCGATGAACGACGAGATCGCGGCGATCGTGCTCC
AGGGCGGCAACGCGATGGACATCGCCAGCGCCGCGCAGAAGATCGGGGTCAAGGACCTGCGCCAGTCGGCCCTGCTCAAG
GCCCGCAACGGCATCACCAGTCTGGCCGAGATCAATCGGGTGACGAAGGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-153)

Type II/IV secretion system protein (T2SSE)

(200-467)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.017

100

0.551
  pilB Acinetobacter baumannii D1279779

54.561

100

0.549
  pilB Legionella pneumophila strain ERS1305867

51.318

98.614

0.506
  pilB Vibrio cholerae strain A1552

49.393

100

0.494
  pilF Neisseria gonorrhoeae MS11

49.472

98.44

0.487
  pilB Vibrio parahaemolyticus RIMD 2210633

46.14

98.787

0.456
  pilB Vibrio campbellii strain DS40M4

45.87

98.614

0.452
  pilB/pilB1 Synechocystis sp. PCC 6803

36.393

100

0.385