Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   NQE15_RS21365 Genome accession   NZ_CP102486
Coordinates   4295007..4295435 (-) Length   142 a.a.
NCBI ID   WP_323054919.1    Uniprot ID   -
Organism   Dechloromonas sp. A34     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 4273332..4294896 4295007..4295435 flank 111


Gene organization within MGE regions


Location: 4273332..4295435
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NQE15_RS21270 - 4273332..4274303 (+) 972 WP_265944520.1 class I SAM-dependent methyltransferase -
  NQE15_RS21275 asnB 4274374..4276191 (+) 1818 WP_416336484.1 asparagine synthase (glutamine-hydrolyzing) -
  NQE15_RS21280 - 4276199..4276897 (+) 699 WP_265944523.1 methyltransferase domain-containing protein -
  NQE15_RS21285 - 4276890..4277711 (+) 822 WP_265944525.1 class I SAM-dependent methyltransferase -
  NQE15_RS21290 - 4277708..4278640 (+) 933 WP_265944527.1 methyltransferase domain-containing protein -
  NQE15_RS21295 - 4278658..4279479 (+) 822 WP_265944529.1 hypothetical protein -
  NQE15_RS21300 - 4280146..4280424 (-) 279 WP_265944531.1 hypothetical protein -
  NQE15_RS21305 - 4280841..4281269 (+) 429 WP_265944532.1 hypothetical protein -
  NQE15_RS21310 - 4281294..4283150 (+) 1857 WP_265944534.1 glycosyltransferase family 2 protein -
  NQE15_RS21315 - 4283147..4284109 (+) 963 WP_265944536.1 glycosyltransferase family 2 protein -
  NQE15_RS21320 - 4284102..4285118 (-) 1017 WP_265944538.1 glycosyltransferase family 2 protein -
  NQE15_RS21325 - 4285115..4286017 (-) 903 WP_265944540.1 NAD-dependent epimerase/dehydratase family protein -
  NQE15_RS21330 - 4286014..4287135 (-) 1122 WP_265944542.1 glycosyltransferase family 4 protein -
  NQE15_RS21335 - 4287186..4288145 (-) 960 WP_265944544.1 glycosyltransferase -
  NQE15_RS21340 - 4288232..4288528 (+) 297 WP_265944546.1 hypothetical protein -
  NQE15_RS21345 - 4288542..4290968 (+) 2427 WP_265944548.1 tetratricopeptide repeat protein -
  NQE15_RS21350 - 4290948..4292936 (-) 1989 WP_265944550.1 acyltransferase family protein -
  NQE15_RS21355 - 4292978..4294441 (-) 1464 WP_265944552.1 hypothetical protein -
  NQE15_RS21360 - 4294474..4294926 (-) 453 WP_265944554.1 hypothetical protein -
  NQE15_RS21365 pilE 4295007..4295435 (-) 429 WP_323054919.1 pilin Machinery gene

Sequence

Protein


Download         Length: 142 a.a.        Molecular weight: 14553.85 Da        Isoelectric Point: 9.0267

>NTDB_id=717258 NQE15_RS21365 WP_323054919.1 4295007..4295435(-) (pilE) [Dechloromonas sp. A34]
MKRVQQGFTLIELMIVVAIIGILAAVALPAYQDYTVRAKVSEVMLAASGPKTTIAEAFQTLGHMPAVASAGISGQTSKYV
ATVAYTTSATNVGTITATASAAEPKIAGSTIVLVGTADATGVVNWDCTTSTIAAKYRPANCR

Nucleotide


Download         Length: 429 bp        

>NTDB_id=717258 NQE15_RS21365 WP_323054919.1 4295007..4295435(-) (pilE) [Dechloromonas sp. A34]
ATGAAGCGTGTACAACAAGGCTTTACCCTGATCGAACTGATGATCGTTGTCGCCATCATCGGTATTCTGGCTGCTGTTGC
GCTGCCGGCTTATCAGGATTACACAGTGCGTGCGAAAGTGTCTGAAGTTATGCTTGCTGCATCCGGCCCAAAAACTACAA
TCGCCGAGGCCTTTCAGACTTTGGGGCATATGCCAGCGGTCGCTTCTGCTGGGATTTCCGGTCAAACATCTAAGTACGTA
GCTACTGTGGCTTATACAACTAGTGCAACGAATGTTGGCACCATCACAGCAACAGCTTCTGCTGCAGAACCGAAAATTGC
CGGTAGCACTATCGTTTTGGTTGGTACCGCCGATGCAACTGGTGTTGTCAATTGGGATTGCACGACGAGCACCATCGCTG
CTAAATATCGTCCTGCCAACTGCCGTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(3-28)

Pilin (bacterial filament) (Pilin)

(36-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

40.526

100

0.542
  pilA2 Legionella pneumophila str. Paris

50.714

98.592

0.5
  pilA2 Legionella pneumophila strain ERS1305867

49.286

98.592

0.486
  comP Acinetobacter baylyi ADP1

47.26

100

0.486
  pilA Ralstonia pseudosolanacearum GMI1000

41.212

100

0.479
  pilE Neisseria gonorrhoeae MS11

40

100

0.465
  pilA/pilAI Pseudomonas stutzeri DSM 10701

44.138

100

0.451
  pilE Neisseria gonorrhoeae strain FA1090

38.272

100

0.437
  pilA/pilAII Pseudomonas stutzeri DSM 10701

40.667

100

0.43
  pilA Pseudomonas aeruginosa PAK

37.013

100

0.401
  pilA Vibrio cholerae C6706

36

100

0.38
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

36

100

0.38
  pilA Vibrio cholerae strain A1552

36

100

0.38
  pilA Acinetobacter baumannii strain A118

37.857

98.592

0.373
  pilA Haemophilus influenzae Rd KW20

36.301

100

0.373