Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NMQ00_RS10880 Genome accession   NZ_CP101462
Coordinates   2094562..2095596 (-) Length   344 a.a.
NCBI ID   WP_255176703.1    Uniprot ID   -
Organism   Exiguobacterium aurantiacum strain CX2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2089562..2100596
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMQ00_RS10860 (NMQ00_10840) pilM 2090233..2091855 (-) 1623 WP_255176699.1 pilus assembly protein PilM -
  NMQ00_RS10865 (NMQ00_10845) - 2091868..2092620 (-) 753 WP_255176700.1 A24 family peptidase -
  NMQ00_RS10870 (NMQ00_10850) - 2092718..2093197 (-) 480 WP_255176701.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  NMQ00_RS10875 (NMQ00_10855) - 2093340..2094557 (-) 1218 WP_255176702.1 type II secretion system F family protein -
  NMQ00_RS10880 (NMQ00_10860) pilT 2094562..2095596 (-) 1035 WP_255176703.1 type IV pilus twitching motility protein PilT Machinery gene
  NMQ00_RS10885 (NMQ00_10865) - 2095601..2097259 (-) 1659 WP_255176704.1 GspE/PulE family protein -
  NMQ00_RS10890 (NMQ00_10870) - 2097261..2097542 (-) 282 WP_215190290.1 hypothetical protein -
  NMQ00_RS10895 (NMQ00_10875) - 2097539..2098765 (-) 1227 WP_255176705.1 G5 domain-containing protein -
  NMQ00_RS10900 (NMQ00_10880) - 2098928..2099380 (+) 453 WP_255176706.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  NMQ00_RS10905 (NMQ00_10885) - 2099319..2099882 (+) 564 WP_215190293.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38317.16 Da        Isoelectric Point: 5.8823

>NTDB_id=710136 NMQ00_RS10880 WP_255176703.1 2094562..2095596(-) (pilT) [Exiguobacterium aurantiacum strain CX2]
MIHQSIEVILTKAVELRASDIHLTAGSPPIIRIDGSLITMGDEKVLPSHIESYLKDIMTDEMRGRLDMQRDLDFSFGVPS
VSRFRVNAYYQRGTVAIAFRSISGEIPTMNELDLPQVLKRLIEKPYGLILVTGPTGSGKSTTLAAMIHEINRTQRKRIIT
LEDPIEYLHSHDQSIVDQREIGSDVMKFVDGLRAALRQDPDVILLGEMRDLETISTAMTAAETGHLVMATLHTSSAMSTV
SRVIDAFPAEQQDQIRTQLANVLLGVVSQRLFPRVDVPKGRVAAMEIMIGNPAVANLIRNEKEFQLPTVIQTNRQNGMQT
MDMAIEQLVRQGVINPNVTPNEER

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=710136 NMQ00_RS10880 WP_255176703.1 2094562..2095596(-) (pilT) [Exiguobacterium aurantiacum strain CX2]
ATGATCCATCAGTCGATTGAAGTGATTTTAACGAAAGCGGTCGAACTACGCGCCTCGGACATCCACTTGACCGCCGGCTC
ACCGCCGATCATCCGGATCGACGGGAGCCTCATCACGATGGGTGACGAGAAAGTGTTGCCGTCTCATATCGAAAGCTATT
TAAAAGACATCATGACGGATGAGATGCGGGGCCGCCTTGATATGCAGCGCGACCTCGATTTCTCGTTCGGCGTCCCGAGC
GTGTCGCGGTTCCGCGTCAACGCCTATTATCAACGAGGTACGGTCGCCATCGCGTTCCGTTCCATTTCCGGGGAAATCCC
GACGATGAACGAGCTCGATTTGCCGCAAGTGTTAAAGCGGTTGATTGAGAAGCCGTACGGCCTCATCCTCGTCACCGGCC
CGACGGGTTCTGGGAAATCGACGACGCTCGCCGCGATGATCCACGAGATTAACCGGACACAGCGGAAACGAATCATCACG
CTCGAAGATCCGATTGAATATCTCCATTCGCACGACCAGAGCATCGTCGATCAACGCGAGATCGGCTCGGACGTCATGAA
GTTCGTCGACGGTCTTCGGGCCGCGCTCCGGCAAGACCCGGACGTCATCTTGCTTGGGGAGATGCGGGATCTCGAGACGA
TTTCGACGGCGATGACGGCTGCCGAGACGGGTCACCTCGTCATGGCAACACTTCATACATCAAGTGCGATGTCGACCGTC
AGCCGTGTCATCGACGCCTTCCCGGCCGAGCAACAGGACCAGATTCGGACCCAGCTCGCCAACGTCCTGCTCGGTGTCGT
CAGTCAGCGCTTATTCCCGCGCGTCGACGTGCCGAAAGGGCGTGTCGCCGCGATGGAAATTATGATCGGCAATCCTGCCG
TCGCCAACTTGATTCGGAACGAGAAGGAGTTCCAACTCCCGACCGTCATCCAGACGAACCGGCAGAACGGGATGCAGACG
ATGGACATGGCAATTGAACAGCTCGTCCGTCAAGGTGTCATCAACCCGAATGTCACACCAAATGAAGAGAGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.92

94.767

0.483
  pilT Acinetobacter baumannii strain A118

48.64

96.221

0.468
  pilT Acinetobacter nosocomialis M2

48.64

96.221

0.468
  pilT Acinetobacter baumannii D1279779

48.64

96.221

0.468
  pilT Pseudomonas aeruginosa PAK

50

92.442

0.462
  pilT Acinetobacter baylyi ADP1

48.318

95.058

0.459
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

47.576

95.93

0.456
  pilT Vibrio cholerae strain A1552

47.576

95.93

0.456
  pilT Neisseria meningitidis 8013

47.13

96.221

0.453
  pilT Neisseria gonorrhoeae MS11

46.809

95.64

0.448
  pilT Pseudomonas stutzeri DSM 10701

48.428

92.442

0.448
  pilT Legionella pneumophila strain Lp02

46.084

96.512

0.445
  pilT Legionella pneumophila strain ERS1305867

46.084

96.512

0.445
  pilU Vibrio cholerae strain A1552

40.244

95.349

0.384
  pilU Pseudomonas stutzeri DSM 10701

39.818

95.64

0.381