Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NLL86_RS01615 Genome accession   NZ_CP100660
Coordinates   364291..365289 (+) Length   332 a.a.
NCBI ID   WP_254482654.1    Uniprot ID   -
Organism   Pseudomonas fluorescens strain ZL22     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 359291..370289
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NLL86_RS01585 (NLL86_01585) - 359756..360190 (-) 435 WP_254482651.1 DUF4426 domain-containing protein -
  NLL86_RS01590 (NLL86_01590) metW 360213..360833 (-) 621 WP_054884211.1 methionine biosynthesis protein MetW -
  NLL86_RS01595 (NLL86_01595) - 360841..361980 (-) 1140 WP_176508103.1 homoserine O-acetyltransferase -
  NLL86_RS01600 (NLL86_01600) - 362080..362670 (-) 591 WP_027920981.1 YggT family protein -
  NLL86_RS01605 (NLL86_01605) proC 362681..363499 (-) 819 WP_254482652.1 pyrroline-5-carboxylate reductase -
  NLL86_RS01610 (NLL86_01610) - 363538..364224 (-) 687 WP_254482653.1 YggS family pyridoxal phosphate-dependent enzyme -
  NLL86_RS01615 (NLL86_01615) pilT 364291..365289 (+) 999 WP_254482654.1 type IV pilus twitching motility protein PilT Machinery gene
  NLL86_RS01620 (NLL86_01620) - 365403..366032 (-) 630 WP_254482655.1 C40 family peptidase -
  NLL86_RS01625 (NLL86_01625) - 366199..366618 (+) 420 WP_254482657.1 TM2 domain-containing protein -
  NLL86_RS01630 (NLL86_01630) - 366675..367376 (-) 702 WP_176508109.1 SPOR domain-containing protein -
  NLL86_RS01635 (NLL86_01635) argS 367378..369114 (-) 1737 WP_099814473.1 arginine--tRNA ligase -

Sequence

Protein


Download         Length: 332 a.a.        Molecular weight: 35896.45 Da        Isoelectric Point: 6.3978

>NTDB_id=706105 NLL86_RS01615 WP_254482654.1 364291..365289(+) (pilT) [Pseudomonas fluorescens strain ZL22]
MDATDLLARAVDAGASDLHLAAGEIPMLRVDGELRRMALPSVTSAAMNDALSALLDEGQCRQWVQGDELDLALELASLGR
FRLSLFRQMNGLAATFRLIPKRIASVDELELEEVFQAVAQHADGLVLVGGPTGSGKSSTLAALIDRLNRERALHIITLED
PVEVVHSSQRSLVNQREIGRHCRDFAQGLRSALRQDPDVIMIGELRDLETVRLALRAAETGHLVLATVHTRSAASSIDRL
VEVFAAEEKPLVRAMLAESLRLVVAQVLVKRGGGGRVAAREVLVATPAVRNLVREGRMAQLCSVMQTGAAEGMRTMEGAM
RGLREKGLISDL

Nucleotide


Download         Length: 999 bp        

>NTDB_id=706105 NLL86_RS01615 WP_254482654.1 364291..365289(+) (pilT) [Pseudomonas fluorescens strain ZL22]
ATGGACGCGACCGACCTGTTGGCCCGGGCCGTGGATGCAGGGGCGAGTGACCTGCATCTGGCGGCGGGCGAGATACCGAT
GCTGCGCGTGGATGGCGAGCTGCGGCGCATGGCGCTGCCAAGCGTGACCTCAGCAGCAATGAACGACGCGCTGTCAGCGC
TGCTCGACGAGGGCCAGTGCCGGCAATGGGTGCAGGGCGATGAACTGGATCTGGCCCTGGAATTGGCCTCTCTGGGGCGT
TTTCGTCTCAGTCTGTTTCGACAGATGAACGGCCTGGCTGCCACGTTTCGTCTGATTCCGAAACGTATCGCCAGTGTCGA
TGAACTCGAACTTGAAGAAGTGTTTCAAGCCGTTGCGCAGCATGCGGATGGCCTGGTGCTGGTGGGCGGCCCGACCGGTA
GTGGCAAGTCGAGTACGCTGGCGGCATTGATCGACCGCCTTAATCGTGAGCGGGCGTTGCACATCATCACTCTTGAAGAC
CCTGTCGAGGTTGTCCACAGCAGCCAACGCAGCCTGGTCAACCAGCGCGAGATCGGCCGGCACTGTCGTGATTTCGCTCA
GGGGCTGCGCAGCGCCCTGCGTCAGGACCCTGATGTGATCATGATCGGCGAACTGCGCGATCTGGAAACCGTCCGCCTGG
CGTTGCGCGCAGCGGAGACAGGGCATCTGGTGCTGGCTACGGTGCATACGCGGTCGGCGGCAAGCAGCATTGACCGGCTG
GTGGAGGTGTTCGCGGCAGAAGAGAAACCCTTGGTGCGGGCGATGCTGGCGGAGTCGTTGAGGTTGGTGGTGGCGCAGGT
GCTGGTCAAGCGTGGGGGCGGCGGCCGAGTGGCTGCGCGAGAAGTGCTGGTGGCGACACCTGCGGTGCGCAATCTGGTGC
GTGAGGGACGGATGGCGCAGTTGTGTTCGGTGATGCAGACCGGGGCGGCGGAGGGGATGCGGACGATGGAAGGGGCGATG
CGGGGGCTGAGGGAGAAGGGACTGATCAGCGATCTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

52.121

99.398

0.518
  pilT Pseudomonas stutzeri DSM 10701

51.212

99.398

0.509
  pilT Acinetobacter baumannii D1279779

50.909

99.398

0.506
  pilT Acinetobacter nosocomialis M2

50.909

99.398

0.506
  pilT Acinetobacter baumannii strain A118

50.909

99.398

0.506
  pilT Acinetobacter baylyi ADP1

50.909

99.398

0.506
  pilT Legionella pneumophila strain Lp02

50.606

99.398

0.503
  pilT Legionella pneumophila strain ERS1305867

50.606

99.398

0.503
  pilT Neisseria meningitidis 8013

49.849

99.699

0.497
  pilT Vibrio cholerae strain A1552

50.152

99.096

0.497
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.152

99.096

0.497
  pilT Neisseria gonorrhoeae MS11

49.547

99.699

0.494
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.015

99.699

0.449
  pilU Pseudomonas stutzeri DSM 10701

36.858

99.699

0.367
  pilU Vibrio cholerae strain A1552

38.71

93.373

0.361