Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LZ581_RS18840 Genome accession   NZ_CP100388
Coordinates   3986449..3987429 (-) Length   326 a.a.
NCBI ID   WP_032669250.1    Uniprot ID   -
Organism   Enterobacter hormaechei subsp. steigerwaltii strain VKH10     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3981449..3992429
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LZ581_RS18810 (LZ581_18810) - 3982327..3982824 (+) 498 WP_015571833.1 SprT family zinc-dependent metalloprotease -
  LZ581_RS18815 (LZ581_18815) endA 3982919..3983626 (+) 708 WP_003860034.1 deoxyribonuclease I -
  LZ581_RS18820 (LZ581_18820) rsmE 3983678..3984409 (+) 732 WP_254309381.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  LZ581_RS18825 (LZ581_18825) gshB 3984441..3985388 (+) 948 WP_015571835.1 glutathione synthase -
  LZ581_RS18830 (LZ581_18830) - 3985463..3986023 (+) 561 WP_006811924.1 YqgE/AlgH family protein -
  LZ581_RS18835 (LZ581_18835) ruvX 3986023..3986439 (+) 417 WP_006811925.1 Holliday junction resolvase RuvX -
  LZ581_RS18840 (LZ581_18840) pilT 3986449..3987429 (-) 981 WP_032669250.1 type IV pilus twitching motility protein PilT Machinery gene
  LZ581_RS18845 (LZ581_18845) - 3987447..3988148 (+) 702 WP_017692690.1 YggS family pyridoxal phosphate-dependent enzyme -
  LZ581_RS18850 (LZ581_18850) - 3988170..3988736 (+) 567 WP_015571838.1 YggT family protein -
  LZ581_RS18855 (LZ581_18855) yggU 3988733..3989029 (+) 297 WP_003860023.1 DUF167 family protein YggU -
  LZ581_RS18860 (LZ581_18860) - 3989033..3989626 (+) 594 WP_015571839.1 XTP/dITP diphosphatase -
  LZ581_RS18865 (LZ581_18865) hemW 3989619..3990767 (+) 1149 WP_017382926.1 radical SAM family heme chaperone HemW -
  LZ581_RS18870 (LZ581_18870) - 3990824..3991162 (+) 339 WP_017382927.1 endonuclease domain-containing protein -
  LZ581_RS18875 (LZ581_18875) - 3991245..3991961 (-) 717 WP_015571842.1 DUF2884 domain-containing protein -
  LZ581_RS18880 (LZ581_18880) - 3992018..3992344 (-) 327 WP_003862421.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35691.71 Da        Isoelectric Point: 6.4131

>NTDB_id=704795 LZ581_RS18840 WP_032669250.1 3986449..3987429(-) (pilT) [Enterobacter hormaechei subsp. steigerwaltii strain VKH10]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRSGRLEPAPFPPPDVEALLKAWLNDEQQGAWWANGQVDFAVTLAGRQRLRG
SAFKHMLGVSIALRLLPLTCPQLSALGVPRAIPELLSNDNGLILVTGATGSGKSTTLAAMVDFLNHHTDGHILTLEDPVE
FMYQSERCLIQQREIGQHSPSFAEALRSALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLRQDVQGGRVALYELLVNTSAAANLIREGKTWQLPGIIQTGQQAGMQNFEQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=704795 LZ581_RS18840 WP_032669250.1 3986449..3987429(-) (pilT) [Enterobacter hormaechei subsp. steigerwaltii strain VKH10]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCGCG
CTGGCGCAGGTCAGGCCGTCTTGAACCTGCGCCGTTTCCGCCCCCGGATGTGGAGGCGTTATTAAAAGCGTGGCTCAACG
ACGAACAGCAGGGCGCATGGTGGGCAAACGGGCAAGTGGATTTTGCCGTTACCCTCGCAGGCCGTCAGCGGCTGCGCGGC
AGTGCGTTTAAGCATATGCTCGGCGTTTCGATTGCGCTGCGGCTGTTGCCGCTGACGTGCCCGCAGCTCTCTGCGCTAGG
TGTGCCGCGAGCGATCCCGGAACTACTGTCCAATGACAATGGCCTGATTCTGGTCACCGGCGCCACCGGCAGTGGGAAAT
CGACCACCCTGGCCGCTATGGTCGATTTCCTCAATCACCATACGGACGGGCATATTCTGACCCTGGAAGATCCGGTGGAG
TTTATGTACCAGAGCGAACGTTGCCTGATCCAGCAGCGGGAGATAGGCCAGCACAGCCCGTCCTTTGCCGAGGCGCTGCG
CAGCGCGCTGCGTGAAGATCCGGATGTGATTCTGCTTGGTGAGCTGCGCGACAGCGAGACGATACGCCTGGCGCTGACGG
CGGCGGAAACCGGCCATCTGGTGTTAGCCACGCTGCACACGCGCGGCGCGGCGCAGGCGATCGAGCGCCTGGTCGACACC
TTTCCGGCGCAGGAGAAAGATCCGGTGCGTAATCAGCTGGCGGGAAGCCTGCGTGCGGTCCTGGCGCAGAAGCTACGTCA
GGATGTCCAGGGCGGGCGCGTGGCGCTGTATGAGCTACTGGTGAATACCTCGGCGGCGGCAAACCTGATCCGCGAAGGCA
AAACCTGGCAACTGCCGGGCATTATTCAAACGGGGCAGCAGGCGGGAATGCAGAACTTTGAGCAGAGTCTGGCGGAGCGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(108-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

50.765

100

0.509
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.765

100

0.509
  pilT Legionella pneumophila strain Lp02

48.758

98.773

0.482
  pilT Legionella pneumophila strain ERS1305867

48.758

98.773

0.482
  pilT Pseudomonas stutzeri DSM 10701

48.012

100

0.482
  pilT Neisseria meningitidis 8013

47.561

100

0.479
  pilT Neisseria gonorrhoeae MS11

47.256

100

0.475
  pilT Acinetobacter baumannii strain A118

47.095

100

0.472
  pilT Acinetobacter baylyi ADP1

47.095

100

0.472
  pilT Acinetobacter baumannii D1279779

47.095

100

0.472
  pilT Pseudomonas aeruginosa PAK

47.095

100

0.472
  pilT Acinetobacter nosocomialis M2

47.5

98.16

0.466
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.344

99.08

0.429
  pilU Pseudomonas stutzeri DSM 10701

38.182

100

0.387
  pilU Vibrio cholerae strain A1552

38.199

98.773

0.377