Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NKE59_RS03620 Genome accession   NZ_CP099959
Coordinates   730774..731784 (+) Length   336 a.a.
NCBI ID   WP_353439615.1    Uniprot ID   A0AAU8A4E8
Organism   Polynucleobacter sp. UK-FUSCHL-C3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 725774..736784
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NKE59_RS03595 (NKE59_03585) - 727230..728195 (+) 966 WP_353439610.1 tripartite tricarboxylate transporter substrate binding protein -
  NKE59_RS03600 (NKE59_03590) - 728238..729143 (+) 906 WP_353439611.1 DMT family transporter -
  NKE59_RS03605 (NKE59_03595) - 729166..729612 (-) 447 WP_353439612.1 hypothetical protein -
  NKE59_RS03610 (NKE59_03600) - 729795..730304 (+) 510 WP_353439613.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  NKE59_RS03615 (NKE59_03605) - 730323..730751 (+) 429 WP_353439614.1 type IV pilin protein -
  NKE59_RS03620 (NKE59_03610) pilT 730774..731784 (+) 1011 WP_353439615.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NKE59_RS03625 (NKE59_03615) - 731789..732334 (+) 546 WP_353439616.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  NKE59_RS03630 (NKE59_03620) - 732315..732812 (+) 498 WP_353439617.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  NKE59_RS03635 (NKE59_03625) - 732816..733469 (+) 654 WP_353439618.1 type II secretion system protein -
  NKE59_RS03640 (NKE59_03630) - 733435..733974 (+) 540 WP_353439619.1 hypothetical protein -
  NKE59_RS03645 (NKE59_03635) - 734055..734426 (+) 372 WP_353439620.1 hypothetical protein -

Sequence

Protein


Download         Length: 336 a.a.        Molecular weight: 37434.10 Da        Isoelectric Point: 5.7557

>NTDB_id=702962 NKE59_RS03620 WP_353439615.1 730774..731784(+) (pilT) [Polynucleobacter sp. UK-FUSCHL-C3]
MWDLFIELAASKKLSDIHLRAGELVAYREYGEIVKTDQTVQQQELLNFLKEELPPEDFAKFLDSRDLDFAAEKKGFRFRI
NAFWADEQIGLVLRKIETSIPNFDTLSLPAYVRTVPSIETGLVLVTGPTGSGKSTTLAAIIDVINQTLAGHIITVEDPVE
FKHPAKRCVVNQREIGRDTKSFLNALRASLREDPDVILVGELRDLETIQLALTAAETGHLVFGTLHTNSAPSTITRIIDV
FPPSQQDQIRSQLSSTLRCVITQRLLKRIDGSGRVGAYEIMICNQAIKNLIRENKVFQIPNVMQTARNEGMMSMEASIRA
LIAEGKVAQDAMLSIT

Nucleotide


Download         Length: 1011 bp        

>NTDB_id=702962 NKE59_RS03620 WP_353439615.1 730774..731784(+) (pilT) [Polynucleobacter sp. UK-FUSCHL-C3]
ATGTGGGATTTGTTCATTGAGTTGGCCGCCTCTAAAAAACTTTCTGATATACATTTAAGGGCGGGGGAACTAGTAGCCTA
TAGGGAATATGGCGAAATCGTAAAAACTGATCAGACAGTCCAGCAACAAGAGTTATTGAATTTCTTAAAAGAAGAGCTAC
CCCCAGAGGATTTTGCAAAATTTTTAGATAGCCGCGATCTAGATTTTGCAGCCGAAAAAAAGGGATTTCGCTTTCGTATT
AATGCCTTTTGGGCGGATGAGCAAATTGGTTTAGTTCTTCGTAAGATCGAAACTTCAATTCCAAACTTTGATACCTTAAG
CCTCCCTGCCTATGTGCGAACAGTTCCAAGTATTGAAACAGGATTAGTTCTTGTTACCGGACCCACAGGTTCAGGTAAAA
GCACTACTTTAGCGGCGATTATCGATGTTATTAATCAAACGTTAGCGGGGCATATTATTACCGTAGAGGATCCTGTTGAA
TTTAAACATCCGGCAAAACGATGTGTGGTTAATCAGCGCGAAATAGGGCGTGATACCAAATCATTCCTAAATGCTTTAAG
AGCCTCATTGCGTGAAGATCCGGACGTTATCTTAGTTGGAGAGCTTCGTGATTTGGAGACCATACAGTTAGCTTTAACTG
CTGCGGAGACTGGTCACCTTGTATTTGGCACATTACACACCAATAGCGCCCCTAGCACTATTACTCGAATAATTGATGTT
TTTCCACCATCACAGCAAGATCAAATTAGATCTCAGCTATCGTCAACCCTTCGATGTGTTATTACCCAACGCTTATTAAA
GCGCATTGATGGCTCAGGTAGAGTCGGCGCCTACGAAATAATGATTTGTAATCAAGCTATCAAAAATCTTATTCGGGAAA
ATAAAGTTTTTCAGATTCCAAATGTGATGCAAACTGCACGAAATGAAGGAATGATGTCTATGGAGGCATCAATCAGAGCG
CTTATTGCGGAGGGCAAAGTGGCTCAAGATGCCATGTTGAGCATCACTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.307

97.024

0.488
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.154

96.726

0.485
  pilT Vibrio cholerae strain A1552

50.154

96.726

0.485
  pilT Pseudomonas aeruginosa PAK

47.432

98.512

0.467
  pilT Pseudomonas stutzeri DSM 10701

47.289

98.81

0.467
  pilT Acinetobacter baylyi ADP1

47.988

96.131

0.461
  pilT Acinetobacter baumannii D1279779

48.418

94.048

0.455
  pilT Acinetobacter baumannii strain A118

48.418

94.048

0.455
  pilT Legionella pneumophila strain ERS1305867

46.789

97.321

0.455
  pilT Legionella pneumophila strain Lp02

46.789

97.321

0.455
  pilT Acinetobacter nosocomialis M2

48.101

94.048

0.452
  pilT Neisseria gonorrhoeae MS11

45.897

97.917

0.449
  pilT Neisseria meningitidis 8013

46.319

97.024

0.449
  pilU Vibrio cholerae strain A1552

39.275

98.512

0.387
  pilU Pseudomonas stutzeri DSM 10701

36.716

99.702

0.366